Deletion of two endo-β-1, 4-xylanase genes reveals additional isozymes secreted by the rice blast fungus

Fungal pathogens secrete hydrolases during infection of plant tissues capable of fragmenting the primary cell wall polysaccharides of the host. Magnaporthe grisea, the fungal pathogen that causes blast disease of graminaceous monocots, secretes two distinct endo-β-1,4-D-xylanases when grown on xylan-rich rice cell walls as the carbon source. We have previously reported the cloning of the genes encoding these two xylanases, XYL1 and XYL2 (formerly XYN22 and XYN33, respectively; see S.-C. Wu, S. Kauffmann, A. G. Darvill, and P. Albersheim, Mol. Plant-Microbe Interact. 8:506–514, 1995). We now present three M. grisea mutants created by selective deletion of XYL1 and/or XYL2. The xyl1 mutant grows as well as the parent in culture medium when rice cell walls or xylan is the sole carbon source. Under the same conditions, the xyl2 mutant grows slightly slower than the parent, whereas the xyl1/xyl2 double mutant exhibits a 50% reduction in accumulation of total mycelial mass. Under conditions idealized for infect...

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