Influence of Different Moisture Contents and Temperature on Growth and Production of Aflatoxin B1 by a Toxigenic Aspergillus flavus Isolate in Wheat Flour

Experimental short time population and production of aflatoxin B1 by Aspergillus flavus in wheat flour at 20, 25, 30, 35 and 40oC, as related to different moisture contents 5%, 8%, 10%, 12%, 14%, 16%, 18%, 20%, 22% and 25%, was studied. Spore suspension of a toxigenic A. flavus containing ~ 3×10 spore/ml was inoculated in each flask containing 50 g wheat flour and incubated for 15 days. Spore count was observed slightly in flour with a moisture content 5% at all incubation temperature, then increased gradually by rising the moisture content reaching the maximum at 30oC with moisture content 25%. High performance liquid chromatography analysis of AFB1 showed the lowest quantification (482 ng/kg) at 20oC with 8% moisture content in comparison to uninoculated control. The higher quantity of AFB1 (637835.4 ng/kg) was produced at 25oC with 25% moisture content. Accumulation of high amounts of AFB1 was noticed at 20, 30 and 35oC with moisture content 25%. However, at 40oC with moisture contents 22% and 25%, high spore count was recorded, but traces of AFB1 were produced. The significance of this investigation is that, in the event of wheat flour contamination with afltoxigenic species, rise in moisture contents are hazardous in the storage of wheat flour around 25oC.

[1]  D. Kovačević,et al.  Contamination of pasta and the raw materials for its production with moulds of the genera Aspergillus , 2018 .

[2]  J. Milani.  Ecological conditions affecting mycotoxin production in cereals: a review. , 2018 .

[3]  Halime Babaie Razdari.  An assessment of Aflatoxin level in wheat samples of 5 top provinces of Iran , 2016 .

[4]  A. El-Sayed,et al.  Detection of aflatoxins by HPLC and the expression of biosynthetic nor-1 gene of aflatoxin and ocrA gene of ochratoxin , 2015 .

[5]  El-Desouky,et al.  Evaluation of ozone gas as an anti-aflatoxin B 1 in wheat grains during storage , 2012 .

[6]  Arijit Das,et al.  Evaluation of Process Parameters Influencing Aflatoxin B1 Synthesis from Aspergillus flavus MTCC 2798 using Rice Straw under Submerged Fermentation , 2012 .

[7]  N. Magan,et al.  A systems approach to model the relationship between aflatoxin gene cluster expression, environmental factors, growth and toxin production by Aspergillus flavus , 2012, Journal of The Royal Society Interface.

[8]  W. Nierman,et al.  Tight control of mycotoxin biosynthesis gene expression in Aspergillus flavus by temperature as revealed by RNA-Seq. , 2011, FEMS microbiology letters.

[9]  D. Gomathi,et al.  Production, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation , 2011 .

[10]  Nelson Lima,et al.  How will climate change affect mycotoxins in food , 2010 .

[11]  N. Magan,et al.  The production of aflatoxin B1 or G1 by Aspergillus parasiticus at various combinations of temperature and water activity is related to the ratio of aflS to aflR expression , 2010, Mycotoxin Research.

[12]  A. M. Pacheco,et al.  Association between aflatoxin and aflatoxigenic fungi in Brazil nut (Bertholletia excelsa H.B.K.) , 2010 .

[13]  Chulze Sn.  Strategies to reduce mycotoxin levels in maize during storage: a review. , 2010 .

[14]  N. Magan,et al.  Complex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperature. , 2009, International journal of food microbiology.

[15]  O. Filtenborg,et al.  Aflatoxin-producing Aspergillus spp. and aflatoxin levels in stored cassava chips as affected by processing practices , 2009 .

[16]  H. Makun,et al.  Fungi and Some Mycotoxins Found in Mouldy Sorghum in Niger State, Nigeria , 2009 .

[17]  Yanni Yin,et al.  Biological control of aflatoxin contamination of crops , 2008, Journal of Zhejiang University SCIENCE B.

[18]  .. M.S.Youssef.  Studies on Mycological Status of Sundried Jew`s-Mallow Leaves and Okra Fruits in Egypt , 2008 .

[19]  F. Tanaka,et al.  Development of a low-moisture-content storage system for brown rice: Storability at decreased moisture contents , 2008 .

[20]  F. Masoero,et al.  Aflatoxins absorption in the gastro-intestinal tract and in the vaginal mucosa in lactating dairy cows , 2008 .

[21]  P. Cotty,et al.  Influences of climate on aflatoxin producing fungi and aflatoxin contamination. , 2007, International journal of food microbiology.

[22]  W. Nierman,et al.  The effect of elevated temperature on gene transcription and aflatoxin biosynthesis , 2007, Mycologia.

[23]  S. Fapohunda,et al.  Studies on Stored cereal degradation by AlternAriA tenuissima , 2006 .

[24]  Jiujiang Yu,et al.  Understanding the genetics of regulation of aflatoxin production and Aspergillus flavus development , 2006, Mycopathologia.

[25]  A. Patriarca,et al.  Effect of water activity and temperature on production of aflatoxin and cyclopiazonic acid by Aspergillus flavus in peanuts. , 2006, Advances in experimental medicine and biology.

[26]  J. D. Biagi,et al.  Fungus and mycotoxins in wheat grain at post harvest. , 2006 .

[27]  Naresh Magan,et al.  Post-Harvest Fungal Ecology: Impact of Fungal Growth and Mycotoxin Accumulation in Stored Grain , 2003, European Journal of Plant Pathology.

[28]  G. Gettinby,et al.  The production of cyclopiazonic acid by Penicillium commune and cyclopiazonic acid and aflatoxins by Aspergillus flavus as affected by water activity and temperature on maize grains , 1996, Mycopathologia.

[29]  E. Ferri,et al.  Minimal moisture content for growth and aflatoxin production by Aspergillus parasiticus in mixed feeds , 1986, Mycopathologia.

[30]  J. M. Soriano,et al.  Determination of aflatoxins in peanuts by matrix solid-phase dispersion and liquid chromatography. , 2003, Journal of chromatography. A.

[31]  A. Hocking,et al.  Microbiology of wheat and flour milling in Australia. , 2003, International journal of food microbiology.

[32]  L. Giannuzzi,et al.  Modelling of aflatoxin production by Aspergillus parasiticus in a solid medium at different temperatures, pH and propionic acid concentrations. , 2002 .

[33]  F J Cabañes,et al.  An easy screening method for fungi producing ochratoxin A in pure culture. , 2001, International journal of food microbiology.

[34]  Ihsan Bakirci,et al.  A study on the occurrence of aflatoxin M1 in milk and milk products produced in Van province of Turkey , 2001 .

[35]  N. Aziz,et al.  INFLUENCE OF MOISTURE CONTENT AND STORAGE TEMPERATURE ON THE PRODUCTION OF AFLATOXIN BY ASPERGILLUS FLAVUS EA‐81 IN MAIZE AFTER EXPOSURE TO GAMMA RADIATION , 1998 .

[36]  M. Vahl,et al.  Determination of aflatoxins in food using LC/MS/MS , 1998 .

[37]  A. El-Gohary.  Study on aflatoxins in some foodstuffs with special reference to public health hazard in Egypt , 1995 .

[38]  L. Bullerman,et al.  Interactive Effects of Spore Load and Temperature on Aflatoxin Production 1. , 1990, Journal of food protection.

[39]  B. Rajagopal,et al.  Aflatoxin production in wheat flour and its effect on protein and carbohydrate content of the flour , 1986 .

[40]  H. V. van Egmond,et al.  Determination of mycotoxins , 1986 .

[41]  E. V. Niles,et al.  Growth and aflatoxin production of Aspergillus flavus in wheat and barley , 1985 .

[42]  R. Hill,et al.  Penicillium species associated with barley grain in the U.K. , 1984 .

[43]  B. Nandi,et al.  Production of Aflatoxin in Rough Rice under Different Storage Conditions , 1984 .

[44]  Kun-Young Park,et al.  Effect of Cycling Temperatures on Aflatoxin Production by Aspergillus parasiticus and Aspergillus flavus in Rice and Cheddar Cheese , 1983 .

[45]  J. Mills.  Insect-fungus associations influencing seed deterioration. , 1983 .

[46]  V. Newcomer.  Laboratory Medical Mycology , 1981 .

[47]  H. V. van Egmond,et al.  Differences Between Aspergillus flavus Strains in Growth and Aflatoxin B1 Production in Relation to Water Activity and Temperature. , 1977, Journal of food protection.

[48]  P. Soentoro,et al.  Effect of Water Activity and Temperature on Aflatoxin Production by Aspergillus parasiticus , 1976 .

[49]  B. Jarvis.  Factors affecting the production of mycotoxins. , 1971 .

[50]  U. L. Diener,et al.  Limiting temperature and relative humidity for aflatoxin production byAspergillus flavus in stored peanuts , 1970, Journal of the American Oil Chemists' Society.

[51]  J. G. Palmer,et al.  Aflatoxin Production by Aspergillus flavus as Related to Various Temperatures. , 1967, Applied microbiology.

[52]  S. Block,et al.  Humidity requirements for mold growth. , 1953, Applied microbiology.