Efforts to install a heterologous Wood-Ljungdahl pathway in Clostridium acetobutylicum enable the identification of the native tetrahydrofolate (THF) cycle and result in early induction of solvents.

[1]  P. Soucaille,et al.  Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum , 2021, Nature Communications.

[2]  Y. Jang,et al.  Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures , 2021, Frontiers in Bioengineering and Biotechnology.

[3]  Shangtian Yang,et al.  Energy-efficient butanol production by Clostridium acetobutylicum with histidine kinase knockouts to improve strain tolerance and process robustness , 2021 .

[4]  J. Keasling,et al.  Biofuels for a sustainable future , 2021, Cell.

[5]  R. Butcher Voices of chemical biology , 2020, Nature Chemical Biology.

[6]  Gi Bae Kim,et al.  Glutaric acid production by systems metabolic engineering of an l-lysine–overproducing Corynebacterium glutamicum , 2020, Proceedings of the National Academy of Sciences.

[7]  S. S. Kachhwaha,et al.  Combustion investigation of ternary blend mixture of biodiesel/n-butanol/diesel: CI engine performance and emission control , 2020 .

[8]  D. Grahame,et al.  Diverse Energy-Conserving Pathways in Clostridium difficile: Growth in the Absence of Amino Acid Stickland Acceptors and the Role of the Wood-Ljungdahl Pathway , 2020, Journal of Bacteriology.

[9]  Liming Liu,et al.  Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia , 2020, Biotechnology for Biofuels.

[10]  Ji Hoon Kim,et al.  Low energy intensity production of fuel-grade bio-butanol enabled by membrane-based extraction , 2020, Energy & Environmental Science.

[11]  Guoqiang Chen,et al.  Engineering biosynthesis of polyhydroxyalkanoates (PHA) for diversity and cost reduction. , 2020, Metabolic engineering.

[12]  Brian F. Pfleger,et al.  Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals. , 2020, Metabolic engineering.

[13]  Tong Un Chae,et al.  Metabolic engineering for the production of dicarboxylic acids and diamines. , 2020, Metabolic engineering.

[14]  Y. Jang,et al.  Metabolic Engineering of Escherichia coli for the Production of Hyaluronic Acid From Glucose and Galactose , 2019, Front. Bioeng. Biotechnol..

[15]  Jae Sung Cho,et al.  A comprehensive metabolic map for production of bio-based chemicals , 2019, Nature Catalysis.

[16]  Ngoc-Phuong-Thao Nguyen,et al.  Reviving the Weizmann process for commercial n-butanol production , 2018, Nature Communications.

[17]  Y. Jang,et al.  Effects of nutritional enrichment on acid production from degenerated (non-solventogenic) Clostridium acetobutylicum strain M5 , 2018, Applied Biological Chemistry.

[18]  E. Papoutsakis,et al.  Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a Novel In Vivo CO Exchange Activity En Route to Heterologous Installation of a Functional Wood-Ljungdahl Pathway , 2018, Applied and Environmental Microbiology.

[19]  H. Koshino,et al.  The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation , 2017, Nature Communications.

[20]  E. Papoutsakis,et al.  Heterologous Expression of the Clostridium carboxidivorans CO Dehydrogenase Alone or Together with the Acetyl Coenzyme A Synthase Enables both Reduction of CO2 and Oxidation of CO by Clostridium acetobutylicum , 2017, Applied and Environmental Microbiology.

[21]  P. Lawson,et al.  Reclassification of Clostridium difficile as Clostridioides difficile (Hall and O'Toole 1935) Prévot 1938. , 2016, Anaerobe.

[22]  P. Soucaille,et al.  Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach , 2016, Biotechnology for Biofuels.

[23]  Philippe Soucaille,et al.  A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum , 2015, mBio.

[24]  Sang Yup Lee,et al.  Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum , 2015, Nature Communications.

[25]  Yong-Su Jin,et al.  Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum , 2015, Proceedings of the National Academy of Sciences.

[26]  D. Rodionov,et al.  Redox-Responsive Repressor Rex Modulates Alcohol Production and Oxidative Stress Tolerance in Clostridium acetobutylicum , 2014, Journal of bacteriology.

[27]  Y. Jang,et al.  Metabolic engineering of Clostridium acetobutylicum for butyric acid production with high butyric acid selectivity. , 2014, Metabolic engineering.

[28]  Martin Fussenegger,et al.  Engineering synergy in biotechnology. , 2014, Nature chemical biology.

[29]  Shangtian Yang,et al.  Engineering Clostridium acetobutylicum with a histidine kinase knockout for enhanced n-butanol tolerance and production , 2014, Applied Microbiology and Biotechnology.

[30]  D. Jahng,et al.  Enhanced butanol production in Clostridium acetobutylicum ATCC 824 by double overexpression of 6-phosphofructokinase and pyruvate kinase genes , 2013, Applied Microbiology and Biotechnology.

[31]  Y. Jang,et al.  Metabolic engineering of Clostridium acetobutylicum for the enhanced production of isopropanol‐butanol‐ethanol fuel mixture , 2013, Biotechnology progress.

[32]  Jingping Liu,et al.  Study on performance and emissions of a passenger-car diesel engine fueled with butanol–diesel blends , 2013 .

[33]  P. Dürre,et al.  Clostridium difficile Is an Autotrophic Bacterial Pathogen , 2013, PloS one.

[34]  F. Dean Toste,et al.  Integration of chemical catalysis with extractive fermentation to produce fuels , 2012, Nature.

[35]  Y. Jang,et al.  Enhanced Butanol Production Obtained by Reinforcing the Direct Butanol-Forming Route in Clostridium acetobutylicum , 2012, mBio.

[36]  Y. Jang,et al.  Bio‐based production of C2–C6 platform chemicals , 2012, Biotechnology and bioengineering.

[37]  H. Bahl,et al.  The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum , 2012, Applied Microbiology and Biotechnology.

[38]  A. Burgard,et al.  Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol. , 2011, Nature chemical biology.

[39]  C. D. Rakopoulos,et al.  Investigation of the performance and emissions of bus engine operating on butanol/diesel fuel blends , 2010 .

[40]  C. D. Rakopoulos,et al.  Effects of butanol–diesel fuel blends on the performance and emissions of a high-speed DI diesel engine , 2010 .

[41]  Serge R. Guiot,et al.  Genomic Analysis of Carbon Monoxide Utilization and Butanol Production by Clostridium carboxidivorans Strain P7T , 2010, PloS one.

[42]  Marion B. Ansorge-Schumacher,et al.  Influence of hydrogenase overexpression on hydrogen production of Clostridium acetobutylicum DSM 792 , 2010 .

[43]  Y. Jang,et al.  Metabolic engineering of Clostridium acetobutylicum M 5 for highly selective butanol production , 2009 .

[44]  U. Sauer,et al.  13C-based metabolic flux analysis , 2009, Nature Protocols.

[45]  E. Papoutsakis,et al.  Aldehyde–alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations , 2009, Biotechnology and bioengineering.

[46]  E. Papoutsakis,et al.  The effect of CO on growth and product formation in batch cultures ofClostridium acetobutylicum , 2005, Biotechnology Letters.

[47]  E. Papoutsakis,et al.  Increased levels of ATP and NADH are associated with increased solvent production in continuous cultures of Clostridium acetobutylicum , 1989, Applied Microbiology and Biotechnology.

[48]  Christoph Wittmann,et al.  Correcting mass isotopomer distributions for naturally occurring isotopes. , 2002, Biotechnology and bioengineering.

[49]  P. Soucaille,et al.  Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824 , 2002, Journal of bacteriology.

[50]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[51]  J. Nielsen,et al.  Metabolic network analysis of Penicillium chrysogenum using (13)C-labeled glucose. , 2000, Biotechnology and bioengineering.

[52]  P. Soucaille,et al.  Solvent-forming genes in clostridia , 1996, Nature.

[53]  E. Papoutsakis,et al.  In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824 , 1993, Applied and environmental microbiology.

[54]  G. Bennett,et al.  Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl-Coenzyme A:Acetate/Butyrate:Coenzyme A-Transferase (EC 2.8.3.9) and in Other Solvent Pathway Enzymes , 1989, Applied and environmental microbiology.

[55]  D. R. Woods,et al.  Acetone-butanol fermentation revisited , 1986 .

[56]  E. Papoutsakis,et al.  The effect of pH on nitrogen supply, cell lysis, and solvent production in fermentations of Clostridium acetobutylicum , 1985, Biotechnology and bioengineering.

[57]  Byung Hong Kim,et al.  Control of Carbon and Electron Flow in Clostridium acetobutylicum Fermentations: Utilization of Carbon Monoxide to Inhibit Hydrogen Production and to Enhance Butanol Yields , 1984, Applied and environmental microbiology.