[17] Time-resolved fluorescence measurements

Nanosecond fluorescence measurements can now be routinely performed and the data can be analyzed in terms of specified decay laws. It is anticipated that nanosecond fluorometry will continue to aid in the elucidation of a variety of excited state mechanistic pathways. This in turn will result in the more sophisticated use of fluorescence probes in biochemistry and cell biology. At the beginning of this decade, Ware9 indicated that the ideal decay fluorometer should permit measurements with samples where the absorbance quantum yield product is 10−10, with high spectral resolution and picosecond time resolution over a wide spectral range. Many of the requirements which seemed so stringent then have now been met. It is likely that the next review on rapid decay techniques will be able to show that picosecond experiments can be carried out with the same degree of confidence that is now possible on the nanosecond time scale.

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