Production of salinity tolerant Nile tilapia, Oreochromis niloticus through traditional and modern breeding methods: II. Application of genetically modified breeding by introducing foreign DNA into fish gonads

This study was conducted to produce a salinity tolerant Nile tilapia, Oreochromis niloticus through genetically modified breeding by introducing a fragmented purified DNA isolated from sea bream, Sparus aurata or Artemia, Artemia salina into the gonads. The results showed a significant improvement (P ≤ 0.05) in most of the growth performance and feed utilization parameters of genetically modified O. niloticus treated with sea bream-DNA and reared at different salinity levels up to 16 ppt compared to both genetically modified O. niloticus treated with Artemia-DNA and the control fish reared at the same salinity levels. Genetically modified O. niloticus treated with Artemia-DNA reared at 32 ppt, had displayed better traits results (P ≤ 0.05) compared to the other fish within the same salinity level. Furthermore, genetically modified O. niloticus treated with sea bream-DNA showed a silver color covering all the body and no dark vertical bands. The results of the random amplified polymorphic DNA (RAPD) fingerprinting showed highly genetic polymorphic percentage (35.95%) among fish receiving foreign DNA and their control using different random primers. The results of the present work suggested that, hyper-saline genetically modified O. niloticus with higher growth rate can be produced using a feasible and fast methodology.

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