Identification and Characterization of Sources of Resistance to Peronospora variabilis in Quinoa

Downy mildew, caused by Peronospora variabilis, is the most important quinoa disease worldwide. However, little is known about the resistance mechanisms acting against this disease. The study goals were to identify quinoa accessions showing resistance to P. variabilis under Spanish field conditions and to characterize the resistance mechanism involved. Towards these objectives, a total amount of 229 accessions of Chenopodium quinoa and one accession of each of the species Chenopodiun berlandieri subs. nutillae, Chenopodium ugandae, and Chenopodium opulifolium were screened for resistance to P. variabilis under field conditions in Córdoba, Spain, during two seasons. The response to P. variabilis in the accessions showed a continuous distribution ranging from complete resistance to high susceptibility. Fifteen resistant and one susceptible accessions were selected for further histological studies. Histological results showed that resistance to downy mildew in quinoa acts mainly at the stage of colony establishment. In resistant accessions, no colonies were formed or success in colony establishment was significantly reduced compared with the susceptible control. Hypersensitive response was associated with colony abortion in a number of the resistant accessions. This work is the first proof of hypersensitive reaction occurrence in quinoa as a response to P. variabilis.

[1]  M. Aydogdu,et al.  Screening quinoa (Chenopodium quinoa) germplasm for resistance to downy mildew (Peronospora variabilis) in Turkey , 2021, Crop and Pasture Science.

[2]  Sandra M. Schmöckel,et al.  Genetic variation for tolerance to the downy mildew pathogen Peronospora variabilis in genetic resources of quinoa (Chenopodium quinoa) , 2020, bioRxiv.

[3]  S. Graeff‐Hönninger,et al.  Quinoa (Chenopodium quinoa Willd.): An Overview of the Potentials of the “Golden Grain” and Socio-Economic and Environmental Aspects of Its Cultivation and Marketization , 2020, Foods.

[4]  P. J. Maughan,et al.  Elevated Genetic Diversity in an F2:6 Population of Quinoa (Chenopodium quinoa) Developed through an Inter-ecotype Cross , 2016, Front. Plant Sci..

[5]  S. Jacobsen,et al.  The Global Expansion of Quinoa: Trends and Limits , 2016, Front. Plant Sci..

[6]  D. Rubiales,et al.  Penetration resistance to Erysiphe pisi in pea mediated by er1 gene is associated with protein cross-linking but not with callose apposition or hypersensitive response , 2015, Euphytica.

[7]  O. Benlhabib,et al.  Assessment of Downy mildew Resistance (Peronospora farinosa) in a Quinoa (Chenopodium quinoa Willd.) Germplasm , 2014 .

[8]  D. Rubiales,et al.  Characterization of mechanisms of resistance against Didymella pinodes in Pisum spp. , 2013, European Journal of Plant Pathology.

[9]  D. Merdinoglu,et al.  Two simplified fluorescent staining techniques to observe infection structures of the oomycete Plasmopara viticola in grapevine leaf tissues. , 2007, Micron.

[10]  Anil . Kumar,et al.  Screening of exotic Chenopodium quinoa accessions for downy mildew resistance under mid-eastern conditions of India , 2006 .

[11]  C. Toker.  Estimates of broad-sense heritability for seed yield and yield criteria in faba bean (Vicia faba L.). , 2004, Hereditas.

[12]  S. Danielsen,et al.  Evaluation of disease assessment methods in quinoa for their ability to predict yield loss caused by downy mildew , 2004 .

[13]  J. Melero-Vara,et al.  Races of Isolates of Plasmopara halstedii from Spain and Studies on Their Virulence. , 2002, Plant disease.

[14]  N. Donofrio,et al.  Abnormal callose response phenotype and hypersusceptibility to Peronospoara parasitica in defence-compromised arabidopsis nim1-1 and salicylate hydroxylase-expressing plants. , 2001, Molecular plant-microbe interactions : MPMI.

[15]  H. D. Frinking,et al.  Postulation of virulence groups and resistance factors in the quinoa/downy mildew pathosystem using material from Ecuador , 1999 .