Polysaccharides in normal and pathologic corneas.

A quantitative method has been developed for the isolation and fractionation of corneal polysaccharides from small tissue samples. Keratan sulfate is molecular weight fractionated and the galactosamine-containing polysaccharides (intermediates between chondroitin and chondroitin-4-sulfate) fractionated on the basis of their sulfate content. Although there is a somexohat lower concentration of the more highly sulfated galactosaminoglycans in the anterior part of the stroma, there is othenvise no significant difference in the quantitative chemical or physicochemical properties of the polysaccharides in the various parts of the corneal stroma. The chemical composition and physicochemical properties of the glucosaminoglycans and. galactosaminoglycans present in early embryonic life differ from those found in adult corneal stroma. A decrease in both glucosaminoglycans and galactosaminoglycans has been demonstrated in the healing area of perforating corneal wounds. The principal polysaccharide constituent of scar tissue seems to be chondroitin sulfate.

[1]  A. Anseth.  Glycosaminoglycans in the developing corneal stroma. , 1961, Experimental eye research.

[2]  A. Anseth.  Glycosaminoglycans in corneal regeneration. , 1961, Experimental eye research.

[3]  A. Anseth.  Studies on corneal polysaccharides. III. Topographic and comparative biochemistry. , 1961, Experimental eye research.

[4]  T. Laurent,et al.  Studies on corneal polysaccharides. II. Characterization. , 1961, Experimental eye research.

[5]  T. Laurent,et al.  Studies on corneal polysaccharides. I. Separation. , 1961, Experimental eye research.

[6]  G. K. Smelser,et al.  Incorporation of S35 in healing wounds in normal and devitalized corneas. , 1958, A.M.A. archives of ophthalmology.

[7]  H. Katzin,et al.  Comparison of bovine corneal and scleral mucopolysaccharides. , 1957, Biochimica et biophysica acta.

[8]  G. K. Smelser,et al.  Distribution of radioactive sulfate in the developing eye. , 1957, American journal of ophthalmology.

[9]  C. Dohlman.  ON THE METABOLISM OF THE CORNEAL GRAFT , 1957, Acta ophthalmologica.

[10]  E. Davidson,et al.  Chondroitin, a new mucopolysaccharide. , 1954, The Journal of biological chemistry.

[11]  G. Aurell.  HEALING PROCESSES IN THE CORNEA WITH SPECIAL REGARD TO STRUCTURE AND METACHROM ASIA , 1954, Acta ophthalmologica.

[12]  E. Davidson,et al.  The mucopolysaccharides of bovine cornea. , 1953, The Journal of biological chemistry.

[13]  I. Werner,et al.  Studies on protein-bound carbohydrates by means of paper partition chromatography. , 1949, Upsala lakareforenings forhandlingar.

[14]  K. Meyer,et al.  The Mucopolysaccharide Acid of the Cornea and Its Enzymatic Hydrolysis , 1940 .

[15]  P. Levene,et al.  MUCINS AND MUCOIDS , 1918 .

[16]  N. Ringertz,et al.  Chromatography on ECTEOLA of Sulfate Containing Mucopolysaccharides. , 1960 .

[17]  G. Larsen.  An autoradiographic study on the uptake of S35-labeled sodium sulfate; in the eyes of normal, scorbutic, and hormonal treated guinea pigs. , 1959, American journal of ophthalmology.

[18]  N. Ringertz,et al.  Chromatography on ECTEOLA of Sulphate-Containing Mucopolysaccharides and Nucleotides. , 1959 .

[19]  G. Smits.  Quantitative interrelationships of the chief components of some connective tissues during foetal and post-natal development in cattle. , 1957, Biochimica et biophysica acta.

[20]  S. Gardell,et al.  Separation of Mucopolysaccharides on a Cellulose Column. , 1957 .

[21]  C. Dohlman.  Incorporation of radioactive sulfate into the rabbit eye. , 1957, Acta ophthalmologica.

[22]  S. Gardell,et al.  Separation on Dowex 50 Ion-Exchange Resin of Glucosamine and Galactosamine and their Quantitative Determination. , 1953 .

[23]  A. Woodin.  The corneal mucopolysaccharide. , 1950, The Biochemical journal.