Supplemental Data Fpr 3 and Zip 3 Ensure that Initiation of Meiotic Recombination Precedes Chromosome Synapsis in Budding Yeast

Strains We replaced the ZIP1 ORF using pEJW1, a ZIP1-GFP integrating plasmid marked with URA3 [1], and then selected for colonies resistant to 5-FOA to isolate a uracil-requiring strain carrying a single copy of ZIP1-GFP. Sporulation-competent haploid strains were constructed by using pB211 to integrate MATa at THR1 in a MATα haploid. fpr3::KAN and dmc1::KAN deletion alleles were constructed using dominant marker gene replacement [2]. The following gene disruptions were previously described: CTF19 tagged with 13-myc [3], zip3::URA3 [4], zip2::LEU2 and ZIP2-GFP:URA3 [5], and spo11::ADE2 [6]. Strains used to measure meiotic gene conversion carried heteroalleles at both HIS4 (his4-260/his4-Hpa) and ARG4 (arg4Nsp/arg4-Bgl).

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