Toxicity of metal oxide nanoparticles: mechanisms, characterization, and avoiding experimental artefacts.

Metal oxide nanomaterials are widely used in practical applications and represent a class of nanomaterials with the highest global annual production. Many of those, such as TiO2 and ZnO, are generally considered non-toxic due to the lack of toxicity of the bulk material. However, these materials typically exhibit toxicity to bacteria and fungi, and there have been emerging concerns about their ecotoxicity effects. The understanding of the toxicity mechanisms is incomplete, with different studies often reporting contradictory results. The relationship between the material properties and toxicity appears to be complex and diifficult to understand, which is partly due to incomplete characterization of the nanomaterial, and possibly due to experimental artefacts in the characterization of the nanomaterial and/or its interactions with living organisms. This review discusses the comprehensive characterization of metal oxide nanomaterials and the mechanisms of their toxicity.

[1]  N. V. von Moos,et al.  Oxidative stress induced by inorganic nanoparticles in bacteria and aquatic microalgae – state of the art and knowledge gaps , 2014, Nanotoxicology.

[2]  A. Djurišić,et al.  Is the effect of surface modifying molecules on antibacterial activity universal for a given material? , 2014, Nanoscale.

[3]  Deborah Berhanu,et al.  Comparative study using spheres, rods and spindle-shaped nanoplatelets on dispersion stability, dissolution and toxicity of CuO nanomaterials , 2014, Nanotoxicology.

[4]  Maria Dusinska,et al.  Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles , 2014, Nanotoxicology.

[5]  M. T. Wong,et al.  Mechanisms of antibacterial activity of MgO: non-ROS mediated toxicity of MgO nanoparticles towards Escherichia coli. , 2014, Small.

[6]  Sören Kaps,et al.  Toxicity of Functional Nano-Micro Zinc Oxide Tetrapods: Impact of Cell Culture Conditions, Cellular Age and Material Properties , 2014, PloS one.

[7]  M. Guedeau-Boudeville,et al.  In vitro toxicity of nanoceria: effect of coating and stability in biofluids , 2013, Nanotoxicology.

[8]  Dries Knapen,et al.  The chronic toxicity of ZnO nanoparticles and ZnCl2 to Daphnia magna and the use of different methods to assess nanoparticle aggregation and dissolution , 2013, Nanotoxicology.

[9]  Rose Amal,et al.  Induced adaptation of Bacillus sp. to antimicrobial nanosilver. , 2013, Small.

[10]  Patrick M. Chong,et al.  A simple shotgun proteomics method for rapid bacterial identification. , 2013, Journal of microbiological methods.

[11]  B. Aliakbarian,et al.  Inactivation of Escherichia coli on anatase and rutile nanoparticles using UV and fluorescent light , 2013 .

[12]  Y. Tseng,et al.  Antibacterial performance of nanoscaled visible-light responsive platinum-containing titania photocatalyst in vitro and in vivo. , 2013, Biochimica et biophysica acta.

[13]  Ji-Eun Kim,et al.  Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation. , 2013, Toxicology in vitro : an international journal published in association with BIBRA.

[14]  F. Gottschalk,et al.  Engineered nanomaterials in water and soils: A risk quantification based on probabilistic exposure and effect modeling , 2013, Environmental toxicology and chemistry.

[15]  Rong Liu,et al.  Nano-SAR development for bioactivity of nanoparticles with considerations of decision boundaries. , 2013, Small.

[16]  S. Ghoshal,et al.  Short-term inactivation rates of selected Gram-positive and Gram-negative bacteria attached to metal oxide mineral surfaces: role of solution and surface chemistry. , 2013, Environmental science & technology.

[17]  A. Ng,et al.  Antibacterial and photocatalytic activity of TiO2 and ZnO nanomaterials in phosphate buffer and saline solution , 2013, Applied Microbiology and Biotechnology.

[18]  C. Muthamizhchelvan,et al.  Morphology-directed synthesis of ZnO nanostructures and their antibacterial activity. , 2013, Colloids and surfaces. B, Biointerfaces.

[19]  Liangzhu Feng,et al.  Graphene oxide-silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms. , 2013, ACS applied materials & interfaces.

[20]  A. Djurišić,et al.  A Comprehensive Review on the Aquatic Toxicity of Engineered Nanomaterials , 2013 .

[21]  P. Poddar,et al.  Probing interaction of gram-positive and gram-negative bacterial cells with ZnO nanorods. , 2013, Materials science & engineering. C, Materials for biological applications.

[22]  W. Heideman,et al.  Influence of humic acid on titanium dioxide nanoparticle toxicity to developing zebrafish. , 2013, Environmental science & technology.

[23]  Y. Konishi,et al.  Exposure of the yeast Saccharomyces cerevisiae to functionalized polystyrene latex nanoparticles: influence of surface charge on toxicity. , 2013, Environmental science & technology.

[24]  J. Field,et al.  Assessing protein oxidation by inorganic nanoparticles with enzyme‐linked immunosorbent assay (ELISA) , 2013, Biotechnology and bioengineering.

[25]  N. Ashbolt,et al.  Legionella pneumophila Transcriptional Response following Exposure to CuO Nanoparticles , 2013, Applied and Environmental Microbiology.

[26]  F. Guyot,et al.  Interaction between Escherichia coli and TiO2 nanoparticles in natural and artificial waters. , 2013, Colloids and surfaces. B, Biointerfaces.

[27]  Kenneth A Dawson,et al.  Nanoparticle adhesion to the cell membrane and its effect on nanoparticle uptake efficiency. , 2013, Journal of the American Chemical Society.

[28]  Dale A Pelletier,et al.  Relating nanomaterial properties and microbial toxicity. , 2013, Nanoscale.

[29]  Chunyang Lei,et al.  Analysis of copper nanoparticles toxicity based on a stress-responsive bacterial biosensor array. , 2013, Nanoscale.

[30]  Paul Pantano,et al.  Generation of toxic degradation products by sonication of Pluronic® dispersants: implications for nanotoxicity testing , 2012, Nanotoxicology.

[31]  A. Ng,et al.  Optical Properties of Oxide Nanomaterials , 2013 .

[32]  M. Mahmoudi,et al.  Erratum: Antibacterial properties of nanoparticles: [Trends in Biotechnology 30 (2012), 499–511] , 2013 .

[33]  Phillip L. Williams,et al.  Ecotoxicity of manufactured ZnO nanoparticles--a review. , 2013, Environmental pollution.

[34]  Fabian Herzog,et al.  Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface , 2013, Particle and Fibre Toxicology.

[35]  N. Tabet,et al.  Effect of the surface texture and crystallinity of ZnO nanoparticles on their toxicity , 2012 .

[36]  A. Ng,et al.  Antibacterial activity of ZnO nanoparticles with a modified surface under ambient illumination , 2012, Nanotechnology.

[37]  A. Bragonzi,et al.  Analysis of Pseudomonas aeruginosa Cell Envelope Proteome by Capture of Surface-Exposed Proteins on Activated Magnetic Nanoparticles , 2012, PloS one.

[38]  J. Posner,et al.  Role of nanoparticle surface functionality in the disruption of model cell membranes. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[39]  Jérôme Labille,et al.  Modifications of the bacterial reverse mutation test reveals mutagenicity of TiO(2) nanoparticles and byproducts from a sunscreen TiO(2)-based nanocomposite. , 2012, Toxicology letters.

[40]  K. Krishnamoorthy,et al.  Mechanistic investigation on the toxicity of MgO nanoparticles toward cancer cells , 2012 .

[41]  M. Lekka,et al.  Photocatalytic and phototoxic properties of TiO2-based nanofilaments: ESR and AFM assays , 2012, Nanotoxicology.

[42]  A. Gedanken,et al.  Understanding the antibacterial mechanism of CuO nanoparticles: revealing the route of induced oxidative stress. , 2012, Small.

[43]  Robert Damoiseaux,et al.  Genome-wide assessment in Escherichia coli reveals time-dependent nanotoxicity paradigms. , 2012, ACS nano.

[44]  E. Ugazio,et al.  Effect of Reactive Species Photogenerated by the Ultraviolet Irradiation of TiO2 on the Peroxidation of Linoleic Acid , 2012 .

[45]  D. E. Aston,et al.  Examination of nanoparticle inactivation of Campylobacter jejuni biofilms using infrared and Raman spectroscopies , 2012, Journal of applied microbiology.

[46]  Christopher M. Hessler,et al.  The influence of capsular extracellular polymeric substances on the interaction between TiO₂ nanoparticles and planktonic bacteria. , 2012, Water research.

[47]  T. Piva,et al.  Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. , 2012, Chemical research in toxicology.

[48]  Vijay Ramani,et al.  CeO2 surface oxygen vacancy concentration governs in situ free radical scavenging efficacy in polymer electrolytes. , 2012, ACS applied materials & interfaces.

[49]  Tian Xia,et al.  Processing pathway dependence of amorphous silica nanoparticle toxicity: colloidal vs pyrolytic. , 2012, Journal of the American Chemical Society.

[50]  Dominique Lison,et al.  Focusing the research efforts. , 2012, Nature nanotechnology.

[51]  Jun Liu,et al.  Phototoxicity of nano titanium dioxides in HaCaT keratinocytes--generation of reactive oxygen species and cell damage. , 2012, Toxicology and applied pharmacology.

[52]  K. Krishnamoorthy,et al.  Antibacterial activity of MgO nanoparticles based on lipid peroxidation by oxygen vacancy , 2012, Journal of Nanoparticle Research.

[53]  N. Loman,et al.  High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity , 2012, Nature Reviews Microbiology.

[54]  P. Munusamy,et al.  Preparation and characterization challenges to understanding environmental and biological impacts of ceria nanoparticles , 2012 .

[55]  Ying Chen,et al.  Surface interactions affect the toxicity of engineered metal oxide nanoparticles toward Paramecium. , 2012, Chemical research in toxicology.

[56]  James F. Ranville,et al.  Silver nanoparticle characterization using single particle ICP-MS (SP-ICP-MS) and asymmetrical flow field flow fractionation ICP-MS (AF4-ICP-MS) , 2012 .

[57]  D. Drobne,et al.  Acclimation of Tetrahymena thermophila to bulk and nano-TiO2 particles by changes in membrane fatty acids saturation. , 2012, Journal of hazardous materials.

[58]  Jing Zhang,et al.  Dissolution and microstructural transformation of ZnO nanoparticles under the influence of phosphate. , 2012, Environmental science & technology.

[59]  Claus-Michael Lehr,et al.  Atomic force microscopy and analytical ultracentrifugation for probing nanomaterial protein interactions. , 2012, ACS nano.

[60]  Y. An,et al.  Effect of ZnO and TiO2 nanoparticles preilluminated with UVA and UVB light on Escherichia coli and Bacillus subtilis , 2012, Applied Microbiology and Biotechnology.

[61]  Yongsheng Chen,et al.  Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. , 2012, ACS nano.

[62]  N. Kabengi,et al.  Can the soil bacterium Cupriavidus necator sense ZnO nanomaterials and aqueous Zn2+ differentially? , 2012, Nanotoxicology.

[63]  Lutz Mädler,et al.  Use of metal oxide nanoparticle band gap to develop a predictive paradigm for oxidative stress and acute pulmonary inflammation. , 2012, ACS nano.

[64]  W. Bai,et al.  Changing exposure media can reverse the cytotoxicity of ceria nanoparticles for Escherichia coli , 2012, Nanotoxicology.

[65]  C. Pagnout,et al.  Role of electrostatic interactions in the toxicity of titanium dioxide nanoparticles toward Escherichia coli. , 2012, Colloids and surfaces. B, Biointerfaces.

[66]  Craig A. Poland,et al.  Zeta potential and solubility to toxic ions as mechanisms of lung inflammation caused by metal/metal oxide nanoparticles. , 2012, Toxicological sciences : an official journal of the Society of Toxicology.

[67]  Yoshihisa Hagihara,et al.  Association of the physical and chemical properties and the cytotoxicity of metal oxide nanoparticles: metal ion release, adsorption ability and specific surface area. , 2012, Metallomics : integrated biometal science.

[68]  W. Stark,et al.  Phosphate starvation as an antimicrobial strategy: the controllable toxicity of lanthanum oxide nanoparticles. , 2012, Chemical communications.

[69]  Verena Wilhelmi,et al.  Evaluation of apoptosis induced by nanoparticles and fine particles in RAW 264.7 macrophages: facts and artefacts. , 2012, Toxicology in vitro : an international journal published in association with BIBRA.

[70]  Xingyu Jiang,et al.  The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli. , 2012, Biomaterials.

[71]  P. Stroeve,et al.  Toxicity of nanomaterials. , 2012, Chemical Society reviews.

[72]  Amit Bandyopadhyay,et al.  Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds. , 2012, Dental materials : official publication of the Academy of Dental Materials.

[73]  V. Hinman,et al.  RNA Deep Sequencing Reveals Differential MicroRNA Expression during Development of Sea Urchin and Sea Star , 2011, PloS one.

[74]  R W Scholz,et al.  Engineered nanomaterials in rivers--exposure scenarios for Switzerland at high spatial and temporal resolution. , 2011, Environmental pollution.

[75]  Juyoung Yoon,et al.  Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species. , 2011, Chemical Society reviews.

[76]  R. Amal,et al.  Cytotoxic origin of copper(II) oxide nanoparticles: comparative studies with micron-sized particles, leachate, and metal salts. , 2011, ACS nano.

[77]  Lauren M. Graham,et al.  Experimental considerations on the cytotoxicity of nanoparticles. , 2011, Nanomedicine.

[78]  M. Suman,et al.  Erratum to: Development of a combined SEM and ICP-MS approach for the qualitative and quantitative analyses of metal microparticles and sub-microparticles in food products , 2011 .

[79]  M. Suman,et al.  Development of a combined SEM and ICP-MS approach for the qualitative and quantitative analyses of metal microparticles and sub-microparticles in food products , 2011, Analytical and bioanalytical chemistry.

[80]  E. Stefanakos,et al.  Lipid vesicles as model membranes in photocatalytic disinfection studies , 2011 .

[81]  Kathleen Wallace,et al.  Proteome profiling reveals potential toxicity and detoxification pathways following exposure of BEAS‐2B cells to engineered nanoparticle titanium dioxide , 2011, Proteomics.

[82]  Andrew P Worth,et al.  A theoretical framework for predicting the oxidative stress potential of oxide nanoparticles , 2011, Nanotoxicology.

[83]  Fadri Gottschalk,et al.  The release of engineered nanomaterials to the environment. , 2011, Journal of environmental monitoring : JEM.

[84]  Andrew P. Worth,et al.  QSAR modeling of nanomaterials. , 2011, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[85]  M. Schäferling,et al.  Luminescent probes for detection and imaging of hydrogen peroxide , 2011 .

[86]  Hans Bouwmeester,et al.  Characterization of translocation of silver nanoparticles and effects on whole-genome gene expression using an in vitro intestinal epithelium coculture model. , 2011, ACS nano.

[87]  K. Jeyasubramanian,et al.  Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation. , 2011, Nanomedicine : nanotechnology, biology, and medicine.

[88]  Ranjit T Koodali,et al.  Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[89]  Anita Jemec,et al.  Ecotoxicity of nanosized TiO2. Review of in vivo data. , 2011, Environmental pollution.

[90]  Jerzy Leszczynski,et al.  Using nano-QSAR to predict the cytotoxicity of metal oxide nanoparticles. , 2011, Nature nanotechnology.

[91]  V. Hackley,et al.  Dispersion stability of nanoparticles in ecotoxicological investigations: the need for adequate measurement tools , 2011 .

[92]  Arturo A. Keller,et al.  Comparative photoactivity of CeO2, γ-Fe2O3, TiO2 and ZnO in various aqueous systems , 2011 .

[93]  Wendelin J Stark,et al.  Nanoparticles in biological systems. , 2011, Angewandte Chemie.

[94]  Peter Wick,et al.  Nanotoxicology: an interdisciplinary challenge. , 2011, Angewandte Chemie.

[95]  Peter L. Irwin,et al.  Antibacterial Activity and Mechanism of Action of Zinc Oxide Nanoparticles against Campylobacter jejuni , 2011, Applied and Environmental Microbiology.

[96]  Lizhong Zhu,et al.  Toxicity of ZnO nanoparticles to Escherichia coli: mechanism and the influence of medium components. , 2011, Environmental science & technology.

[97]  Indran Amirthanayagam,et al.  Lead , 2006, Pediatric Environmental Health.

[98]  Xiaomu Lu,et al.  Recent developments in the detection of singlet oxygen with molecular spectroscopic methods , 2011 .

[99]  O. Yurchenko,et al.  Ultrastructural and some functional changes in tumor cells treated with stabilized iron oxide nanoparticles. , 2010, Experimental oncology.

[100]  Roberto Cingolani,et al.  Effects of cell culture media on the dynamic formation of protein-nanoparticle complexes and influence on the cellular response. , 2010, ACS nano.

[101]  R. Vachet,et al.  Interaction between oxide nanoparticles and biomolecules of the bacterial cell envelope as examined by infrared spectroscopy. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[102]  Soohee Kim,et al.  Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish. , 2010, Aquatic toxicology.

[103]  J. Hong,et al.  In vivo electron spin resonance: An effective new tool for reactive oxygen species/reactive nitrogen species measurement , 2010, Archives of pharmacal research.

[104]  Charalambos Kaittanis,et al.  Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles. , 2010, ACS nano.

[105]  Alok Dhawan,et al.  Toxicity assessment of nanomaterials: methods and challenges , 2010, Analytical and bioanalytical chemistry.

[106]  C. Tyler,et al.  Review: Do engineered nanoparticles pose a significant threat to the aquatic environment? , 2010, Critical reviews in toxicology.

[107]  Alexandra Kroll,et al.  Testing Metal‐Oxide Nanomaterials for Human Safety , 2010, Advanced materials.

[108]  M. Kohno.  Applications of Electron Spin Resonance Spectrometry for Reactive Oxygen Species and Reactive Nitrogen Species Research , 2010, Journal of clinical biochemistry and nutrition.

[109]  Vicki Stone,et al.  Intracellular imaging of nanoparticles: Is it an elemental mistake to believe what you see? , 2010, Particle and Fibre Toxicology.

[110]  Paul Quincey,et al.  Effect of nanoparticle concentration on zeta-potential measurement results and reproducibility , 2010 .

[111]  Vicki Stone,et al.  Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. , 2010, Toxicological sciences : an official journal of the Society of Toxicology.

[112]  J. Chorover,et al.  ATR-FTIR studies of phospholipid vesicle interactions with alpha-FeOOH and alpha-Fe2O3 surfaces. , 2010, Colloids and surfaces. B, Biointerfaces.

[113]  L. Dai,et al.  Preparation of cells for assessing ultrastructural localization of nanoparticles with transmission electron microscopy , 2010, Nature Protocols.

[114]  B. Quinn,et al.  Electrostatic interactions affect nanoparticle-mediated toxicity to gram-negative bacterium Pseudomonas aeruginosa PAO1. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[115]  Amanda S Barnard,et al.  One-to-one comparison of sunscreen efficacy, aesthetics and potential nanotoxicity. , 2010, Nature nanotechnology.

[116]  John M. Veranth,et al.  ZnO particulate matter requires cell contact for toxicity in human colon cancer cells. , 2010, Chemical research in toxicology.

[117]  K. Linge,et al.  Bioavailability of nanoscale metal oxides TiO(2), CeO(2), and ZnO to fish. , 2010, Environmental science & technology.

[118]  N. Dimitrijević,et al.  Interfacial charge transfer between CdTe quantum dots and gram negative vs gram positive bacteria. , 2010, Environmental science & technology.

[119]  S. Oldenburg,et al.  Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro , 2009, Environmental health perspectives.

[120]  Lang Tran,et al.  Evaluating the uptake and intracellular fate of polystyrene nanoparticles by primary and hepatocyte cell lines in vitro. , 2010, Toxicology and applied pharmacology.

[121]  A. Djurišić,et al.  Toxicities of nano zinc oxide to five marine organisms: influences of aggregate size and ion solubility , 2010, Analytical and bioanalytical chemistry.

[122]  Vicki Stone,et al.  Identification of the mechanisms that drive the toxicity of TiO2 particulates: the contribution of physicochemical characteristics , 2009, Particle and Fibre Toxicology.

[123]  Wei Liu,et al.  Toxicity and penetration of TiO2 nanoparticles in hairless mice and porcine skin after subchronic dermal exposure. , 2009, Toxicology letters.

[124]  Y. Vladimirov,et al.  Free radicals and cell chemiluminescence , 2009, Biochemistry (Moscow).

[125]  Christie M. Sayes,et al.  The relationship between pH and zeta potential of ∼ 30 nm metal oxide nanoparticle suspensions relevant to in vitro toxicological evaluations , 2009 .

[126]  Jongheop Yi,et al.  Evaluation of the toxic impact of silver nanoparticles on Japanese medaka (Oryzias latipes). , 2009, Aquatic toxicology.

[127]  Hao Li,et al.  Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157:H7 , 2009, Journal of applied microbiology.

[128]  Jeffrey I Ellis,et al.  The safety of nanosized particles in titanium dioxide- and zinc oxide-based sunscreens. , 2009, Journal of the American Academy of Dermatology.

[129]  Tetsuo Nagano,et al.  Bioimaging Probes for Reactive Oxygen Species and Reactive Nitrogen Species , 2009, Journal of clinical biochemistry and nutrition.

[130]  Harry Friedmann,et al.  EPR Study of Visible Light-Induced ROS Generation by Nanoparticles of ZnO , 2009 .

[131]  S. Agarwal,et al.  Delineating Bacteriostatic and Bactericidal Targets in Mycobacteria Using IPTG Inducible Antisense Expression , 2009, PloS one.

[132]  Alexandra Kroll,et al.  Current in vitro methods in nanoparticle risk assessment: limitations and challenges. , 2009, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[133]  J. Lead,et al.  High doses of intravenously administered titanium dioxide nanoparticles accumulate in the kidneys of rainbow trout but with no observable impairment of renal function. , 2009, Toxicological sciences : an official journal of the Society of Toxicology.

[134]  Mark R Wiesner,et al.  Comparative photoactivity and antibacterial properties of C60 fullerenes and titanium dioxide nanoparticles. , 2009, Environmental science & technology.

[135]  Wei Jiang,et al.  Bacterial toxicity comparison between nano- and micro-scaled oxide particles. , 2009, Environmental pollution.

[136]  K. Paknikar,et al.  Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells. , 2009, Toxicology and applied pharmacology.

[137]  Catalin C. Barbacioru,et al.  mRNA-Seq whole-transcriptome analysis of a single cell , 2009, Nature Methods.

[138]  Jongheop Yi,et al.  Ecotoxicity of silver nanoparticles on the soil nematode Caenorhabditis elegans using functional ecotoxicogenomics. , 2009, Environmental science & technology.

[139]  Rachel Lubart,et al.  Enhanced Antibacterial Activity of Nanocrystalline ZnO Due to Increased ROS‐Mediated Cell Injury , 2009 .

[140]  Haijiao Zhang,et al.  Nanosized zinc oxide particles induce neural stem cell apoptosis , 2009, Nanotechnology.

[141]  Markus Schulz,et al.  Genotoxicity investigations on nanomaterials: methods, preparation and characterization of test material, potential artifacts and limitations--many questions, some answers. , 2009, Mutation research.

[142]  C. McCullagh,et al.  Variables to be considered when assessing the photocatalytic destruction of bacterial pathogens. , 2009, Chemosphere.

[143]  Bryce J Marquis,et al.  Analytical methods to assess nanoparticle toxicity. , 2009, The Analyst.

[144]  Chao Liu,et al.  Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition , 2009, Journal of applied toxicology : JAT.

[145]  S. Doak,et al.  NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. , 2009, Biomaterials.

[146]  M. Gerstein,et al.  RNA-Seq: a revolutionary tool for transcriptomics , 2009, Nature Reviews Genetics.

[147]  J. Schlager,et al.  DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. , 2008, Toxicology and applied pharmacology.

[148]  Michael V. Liga,et al.  Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. , 2008, Water research.

[149]  R. L. Jones,et al.  Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. , 2008, The journal of physical chemistry. B.

[150]  Bin Jiang,et al.  Functional characterisation of altered outer membrane proteins for tetracycline resistance in Escherichia coli. , 2008, International journal of antimicrobial agents.

[151]  Benjamin Gilbert,et al.  Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. , 2008, ACS nano.

[152]  P. Bowen,et al.  Inactivation of E. coli mediated by high surface area CuO accelerated by light irradiation >360 nm , 2008 .

[153]  Jamie R Lead,et al.  Nanomaterials in the environment: Behavior, fate, bioavailability, and effects , 2008, Environmental toxicology and chemistry.

[154]  H. Karlsson,et al.  Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes. , 2008, Chemical research in toxicology.

[155]  Jamie R Lead,et al.  Manufactured nanoparticles: an overview of their chemistry, interactions and potential environmental implications. , 2008, The Science of the total environment.

[156]  A. Ivask,et al.  Biotests and Biosensors for Ecotoxicology of Metal Oxide Nanoparticles: A Minireview , 2008, Sensors.

[157]  W. K. Chan,et al.  Antibacterial activity of ZnO nanorods prepared by a hydrothermal method , 2008 .

[158]  Agnes B Kane,et al.  Adsorption of essential micronutrients by carbon nanotubes and the implications for nanotoxicity testing. , 2008, Small.

[159]  A. Neal,et al.  What can be inferred from bacterium–nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles? , 2008, Ecotoxicology.

[160]  Mark R Wiesner,et al.  Antibacterial activity of fullerene water suspensions (nC60) is not due to ROS-mediated damage. , 2008, Nano letters.

[161]  Awadhesh N Jha,et al.  Hydroxyl radicals (*OH) are associated with titanium dioxide (TiO(2)) nanoparticle-induced cytotoxicity and oxidative DNA damage in fish cells. , 2008, Mutation research.

[162]  Anne Kahru,et al.  Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus. , 2008, Chemosphere.

[163]  Mark Crane,et al.  The ecotoxicology and chemistry of manufactured nanoparticles , 2008, Ecotoxicology.

[164]  Yunqing Kang,et al.  Toxicological effect of ZnO nanoparticles based on bacteria. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[165]  Julian Moger,et al.  Imaging metal oxide nanoparticles in biological structures with CARS microscopy. , 2008, Optics express.

[166]  Saber M Hussain,et al.  Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. , 2008, Toxicological sciences : an official journal of the Society of Toxicology.

[167]  David B Warheit,et al.  How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization? , 2008, Toxicological sciences : an official journal of the Society of Toxicology.

[168]  G. E. Gadd,et al.  Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata): the importance of particle solubility. , 2007, Environmental science & technology.

[169]  David M. Brown,et al.  Measurement of reactive species production by nanoparticles prepared in biologically relevant media. , 2007, Toxicology letters.

[170]  Sudipta Seal,et al.  Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential. , 2007, Biomaterials.

[171]  S. K. Sundaram,et al.  Adsorbed proteins influence the biological activity and molecular targeting of nanomaterials. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[172]  P. Wardman,et al.  Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects. , 2007, Free radical biology & medicine.

[173]  Michael Aschner,et al.  Effects of Nanoparticles on the Adhesion and Cell Viability on Astrocytes , 2007, Biological Trace Element Research.

[174]  Rebecca Klaper,et al.  Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions (titanium dioxide, nano-C60, and C60HxC70Hx). , 2007, Environmental science & technology.

[175]  Paramvir S. Dehal,et al.  Cell-Wide Responses to Low-Oxygen Exposure in Desulfovibrio vulgaris Hildenborough , 2007, Journal of bacteriology.

[176]  Yulong Ding,et al.  Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids) , 2007 .

[177]  S. Hallin,et al.  Silver (Ag+) reduces denitrification and induces enrichment of novel nirK genotypes in soil. , 2007, FEMS microbiology letters.

[178]  L. Murr,et al.  Cytotoxic effects of aggregated nanomaterials. , 2007, Acta biomaterialia.

[179]  Robert N Grass,et al.  Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress. , 2007, Environmental science & technology.

[180]  G. Sayler,et al.  Attributing Effects of Aqueous C60 Nano-Aggregates to Tetrahydrofuran Decomposition Products in Larval Zebrafish by Assessment of Gene Expression , 2007, Environmental health perspectives.

[181]  Sara Linse,et al.  Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles , 2007, Proceedings of the National Academy of Sciences.

[182]  Chul-Hyun Kim,et al.  Charge-associated effects of fullerene derivatives on microbial structural integrity and central metabolism. , 2007, Nano letters.

[183]  John C Crittenden,et al.  Enhanced bioaccumulation of cadmium in carp in the presence of titanium dioxide nanoparticles. , 2007, Chemosphere.

[184]  André R Studart,et al.  Colloidal stabilization of nanoparticles in concentrated suspensions. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[185]  Z. Chai,et al.  Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. , 2007, Toxicology letters.

[186]  G. G. Leppard,et al.  Strategies and Advances in the Characterisation of Environmental Colloids by Electron Microscopy Denis Mavrocordatos , 2007 .

[187]  Pedro J J Alvarez,et al.  Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. , 2006, Water research.

[188]  Jinxue Guo,et al.  Dependence of the cytotoxicity of multi-walled carbon nanotubes on the culture medium , 2006, Nanotechnology.

[189]  Nicolas H Voelcker,et al.  Evaluation of mammalian cell adhesion on surface-modified porous silicon. , 2006, Biomaterials.

[190]  Franck Chauvat,et al.  Cytotoxicity of CeO2 nanoparticles for Escherichia coli. Physico-chemical insight of the cytotoxicity mechanism. , 2006, Environmental science & technology.

[191]  R. Aitken,et al.  Manufacture and use of nanomaterials: current status in the UK and global trends. , 2006, Occupational medicine.

[192]  Oleg M. Sarkisov,et al.  Laser kinetic spectroscopy of the interfacial charge transfer between membrane cell walls of E. coli and TiO2 , 2006 .

[193]  Mark R Wiesner,et al.  Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. , 2006, Nano letters.

[194]  J. West,et al.  Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[195]  Y. Nosaka,et al.  Electron spin resonance studies on the oxidation mechanism of sterically hindered cyclic amines in TiO2 photocatalytic systems. , 2006, The journal of physical chemistry. B.

[196]  Navid B. Saleh,et al.  Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity. , 2006, Environmental science & technology.

[197]  G. Bartosz.  Use of spectroscopic probes for detection of reactive oxygen species. , 2006, Clinica chimica acta; international journal of clinical chemistry.

[198]  N. Soh.  Recent advances in fluorescent probes for the detection of reactive oxygen species , 2006, Analytical and bioanalytical chemistry.

[199]  M. Thyssen,et al.  The bactericidal effect of TiO2 photocatalysis involves adsorption onto catalyst and the loss of membrane integrity. , 2006, FEMS microbiology letters.

[200]  H. Krug,et al.  Oops they did it again! Carbon nanotubes hoax scientists in viability assays. , 2006, Nano letters.

[201]  P. Bowen,et al.  Catalytic activity of commercial of TiO2 powders for the abatement of the bacteria (E. coli) under solar simulated light: Influence of the isoelectric point , 2006 .

[202]  Chi-Ming Che,et al.  Proteomic analysis of the mode of antibacterial action of silver nanoparticles. , 2006, Journal of proteome research.

[203]  M. Benedetti,et al.  Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. , 2006, Nano letters.

[204]  Geoffrey Punshon,et al.  Interactions between endothelial cells and a poly(carbonate-silsesquioxane-bridge-urea)urethane. , 2005, Biomaterials.

[205]  Raz Jelinek,et al.  Microwave‐Assisted Synthesis of Nanocrystalline MgO and Its Use as a Bacteriocide , 2005 .

[206]  J. Gearhart,et al.  In vitro toxicity of nanoparticles in BRL 3A rat liver cells. , 2005, Toxicology in vitro : an international journal published in association with BIBRA.

[207]  Ling Yang,et al.  Particle surface characteristics may play an important role in phytotoxicity of alumina nanoparticles. , 2005, Toxicology letters.

[208]  V. Nadtochenko,et al.  Evidence for the mechanism of photocatalytic degradation of the bacterial wall membrane at the TiO2 interface by ATR-FTIR and laser kinetic spectroscopy. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[209]  W. Dauber,et al.  The Transillumination Possibility of Imidazole–Osmium Postfixed Tissue and Its Consequences for the Handling of Tissue Samples , 2005, Microscopy and Microanalysis.

[210]  Jiaguo Yu,et al.  Efficient visible-light-induced photocatalytic disinfection on sulfur-doped nanocrystalline titania. , 2005, Environmental science & technology.

[211]  V. Nadtochenko,et al.  Dynamics of E. coli membrane cell peroxidation during TiO2 photocatalysis studied by ATR-FTIR spectroscopy and AFM microscopy , 2005 .

[212]  V. Nadtochenko,et al.  New Evidence for TiO2 Photocatalysis during Bilayer Lipid Peroxidation , 2004 .

[213]  Cesar Pulgarin,et al.  Effect of pH, inorganic ions, organic matter and H2O2 on E. coli K12 photocatalytic inactivation by TiO2: Implications in solar water disinfection , 2004 .

[214]  Jay L Zweier,et al.  Detection of reactive oxygen and nitrogen species by EPR spin trapping. , 2004, Antioxidants & redox signaling.

[215]  A. Curtis,et al.  Lactoferrin and ceruloplasmin derivatized superparamagnetic iron oxide nanoparticles for targeting cell surface receptors. , 2004, Biomaterials.

[216]  Vincent M Rotello,et al.  Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. , 2004, Bioconjugate chemistry.

[217]  Shoko Yoshida,et al.  Fluorescent probes for hydrogen peroxide based on a non-oxidative mechanism. , 2004, Angewandte Chemie.

[218]  E. Oberdörster.  Manufactured Nanomaterials (Fullerenes, C60) Induce Oxidative Stress in the Brain of Juvenile Largemouth Bass , 2004, Environmental health perspectives.

[219]  Wonyong Choi,et al.  Linear correlation between inactivation of E. coli and OH radical concentration in TiO2 photocatalytic disinfection. , 2004, Water research.

[220]  K. Bunai,et al.  Profiling and comprehensive expression analysis of ABC transporter solute‐binding proteins of Bacillus subtilis membrane based on a proteomic approach , 2004, Electrophoresis.

[221]  Alexey I Nesvizhskii,et al.  Initial Proteome Analysis of Model Microorganism Haemophilus influenzae Strain Rd KW20 , 2003, Journal of bacteriology.

[222]  M. Kosmulski.  A literature survey of the differences between the reported isoelectric points and their discussion , 2003 .

[223]  Tetsuaki Tsuchido,et al.  Mode of Bactericidal Action of Silver Zeolite and Its Comparison with That of Silver Nitrate , 2003, Applied and Environmental Microbiology.

[224]  Y. Urano,et al.  Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species* 210 , 2003, The Journal of Biological Chemistry.

[225]  K. Klabunde,et al.  Metal Oxide Nanoparticles as Bactericidal Agents , 2002 .

[226]  M. Kanehisa,et al.  Whole genome sequencing of meticillin-resistant Staphylococcus aureus , 2001, The Lancet.

[227]  R. Anken,et al.  Vesicular bodies in fish maculae are artifacts not contributing to otolith growth , 2001, Hearing Research.

[228]  J. Sawai,et al.  Antibacterial characteristics of magnesium oxide powder , 2000 .

[229]  Edward J. Wolfrum,et al.  Bactericidal Activity of Photocatalytic TiO2 Reaction: toward an Understanding of Its Killing Mechanism , 1999, Applied and Environmental Microbiology.

[230]  G. Voordouw,et al.  Deletion of the rbo Gene Increases the Oxygen Sensitivity of the Sulfate-Reducing BacteriumDesulfovibrio vulgaris Hildenborough , 1998, Applied and Environmental Microbiology.

[231]  Akira Fujishima,et al.  Photocatalytic bactericidal effect of TiO2 thin films : dynamic view of the active oxygen species responsible for the effect , 1997 .

[232]  T. M. Bradley,et al.  Cysteine-to-alanine replacements in the Escherichia coli SoxR protein and the role of the [2Fe-2S] centers in transcriptional activation. , 1997, Nucleic acids research.

[233]  J. LaRoche,et al.  Estimating the Growth Rate of Slowly Growing Marine Bacteria from RNA Content , 1993, Applied and environmental microbiology.

[234]  B. Halliwell,et al.  Lipid peroxidation: its mechanism, measurement, and significance. , 1993, The American journal of clinical nutrition.