Lamina propria macrophages in the human gastrointestinal mucosa: their distribution, immunohistological phenotype, and function.

In this study we systematically investigated the cellular distribution, immunohistochemical phenotype, and mucosal disposal function of macrophages in the lamina propria of the human gastrointestinal mucosa (lamina propria macrophages; LPMs). In all tissues examined, most of these LPMs accumulated beneath the epithelial layer that covered the apex of the lamina propria of the mucosa. These cells expressed normal levels of common macrophage markers such as CD68, LN5, lysozyme, ferritin, and alpha 1-anti-chymotrypsin. In addition, they expressed high levels of 25F9 (a market for a certain subpopulation of macrophages), MHC Class II molecules, and CD74 (MHC Class II-associated invariant chain). Interestingly, LPMs possessed some epithelial cell-associated antigens such as cytokeratin, carcinoembryonic antigen (CEA), and Ber-Ep4 in their cytoplasm. Ultrastructurally, these antigens were associated with cellular debris ingested by LPMs, which were recognized as apoptotic fragments by in situ end-labeling. Furthermore, double positive-labeled granules were seen in LPMs by double staining for epithelial cell-associated antigens and in situ end-labeling. These observations suggest that one of the major functions of LPMs is the disposal of apoptotic epithelial cells and that LPMs may be involved in the regulation of mucosal epithelial renewal.

[1]  Johannes Gerdes,et al.  Antigen unmasking on formalin‐fixed, paraffin‐embedded tissue sections , 1993, The Journal of pathology.

[2]  L. Mayer,et al.  A novel human macrophage-derived intestinal mucin secretagogue: implications for the pathogenesis of inflammatory bowel disease. , 1993, Gastroenterology.

[3]  P. Buckley,et al.  Macrophages (histiocytes) in various reactive and inflammatory conditions express different antigenic phenotypes. , 1992, Human pathology.

[4]  Fujita Tsuneo,et al.  Macrophages Possibly Involved in the Disposal of ApoptoticEpithelial Cells in the Monkey Small and Large Intestine , 1992 .

[5]  D. Conrad,et al.  Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII). , 1992, Journal of immunology.

[6]  P. Bland,et al.  Antigen handling by the epithelium and lamina propria macrophages. , 1991, Gastroenterology clinics of North America.

[7]  D. Jones,et al.  Differential expression of MHC- and macrophage-associated antigens in human fetal and postnatal small intestine. , 1990, Immunology.

[8]  D. Jewell,et al.  Effect of human intestinal macrophages on immunoglobulin production by human intestinal mononuclear cells isolated from patients with inflammatory bowel disease , 1990, Clinical and experimental immunology.

[9]  Y. Mahida,et al.  Characterization of antigen-presenting activity of intestinal mononuclear cells isolated from normal and inflammatory bowel disease colon and ileum. , 1988, Immunology.

[10]  H. Verspaget,et al.  Disordered regulation of the in vitro immunoglobulin synthesis by intestinal mononuclear cells in Crohn's disease. , 1988, Gut.

[11]  J. Strominger,et al.  Interaction between CD4 and class II MHC molecules mediates cell adhesion , 1987, Nature.

[12]  D. Hume,et al.  Immunohistochemical Characterisation of Macrophages in Human Liver and Gastrointestinal Tract: Expression of CD4, HLA‐DR, OKM1, and the Mature Macrophage Marker 25F9 in Normal and Diseased Tissue , 1987, Journal of leukocyte biology.

[13]  M. Smith,et al.  Human spleen contains phenotypic subsets of macrophages and dendritic cells that occupy discrete microanatomic locations. , 1987, The American journal of pathology.

[14]  E. Bröcker,et al.  A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. , 1985, Journal of immunology.

[15]  D. Jewell,et al.  Heterogeneity of HLA-DR-positive histiocytes in human intestinal lamina propria: a combined histochemical and immunohistological analysis. , 1983, Journal of clinical pathology.

[16]  D. Jewell,et al.  T lymphocyte subsets in human intestinal mucosa: the distribution and relationship to MHC-derived antigens. , 1981, Clinical and experimental immunology.

[17]  W. Sawicki,et al.  Lamina propria macrophages of intestine of the guinea pig. Possible role in phagocytosis of migrating cells. , 1977, Gastroenterology.

[18]  G. Eastwood,et al.  Gastrointestinal epithelial renewal. , 1977, Gastroenterology.

[19]  E. Yunis,et al.  Macrophages of the rectal lamina propria in children. , 1970, American Journal of Clinical Pathology.

[20]  M. Lipkin,et al.  Cell proliferation kinetics in the gastrointestinal tract of man. 3. Cell renewal in esophagus, stomach, and jejunum of a patient with treated pernicious anemia. , 1967, Journal of the National Cancer Institute.

[21]  M. Karnovsky,et al.  THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE , 1966, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[22]  W. Donnellan,et al.  The structure of the colonic mucosa. The epithelium and subepithelial reticulohistiocytic complex. , 1965, Gastroenterology.

[23]  N. Walker,et al.  Ultrastructure Of Human Melanosis Coli With Reference To Its Pathogenesis , 1993, Pathology.

[24]  D. Hume,et al.  Dendritic cells, the major antigen-presenting cells of the human colonic lamina propria. , 1993, Immunology.

[25]  F. Brodsky,et al.  The cell biology of antigen processing and presentation. , 1991, Annual review of immunology.

[26]  H. Kawanishi,et al.  Bacillary characteristics in Whipple's disease: an electron microscopic study. , 1981, Gastroenterology.

[27]  M. Dickman,et al.  The Microbial ecology of the upper small bowel. , 1976, The American journal of gastroenterology.

[28]  H Cheng,et al.  Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. II. Mucous cells. , 1974, The American journal of anatomy.