Expression of cathepsins B and S in the progression of prostate carcinoma

Cathepsins B and S (CatB, CatS) are lysosomal cysteine proteases which, among other functions, appear to play a role in cancer progression in different tumor models due to their matrix‐degrading properties. To investigate their possible involvement in the development of prostate carcinoma, we immunohistochemically analyzed CatB and CatS in 38 primary human prostatic adenocarcinomas, as well as concomitant high‐grade prostatic intra‐epithelial neoplasia, nodular hyperplasia and normal tissue. CatB expression was observed in 28 (74%) and CatS in 32 (84%) carcinomas, being concomitant in 24 cases (63%). High‐grade intra‐epithelial neoplasia expressed CatB in 20/23 cases (87%), and a similar result was obtained for CatS, with expression of both coinciding in 18 cases (78%). In non‐neoplastic tissue, strong expression of both proteases was observed in macrophages, inflamed glands and transitional metaplasia, whereas atrophic glands and basal cells of normal glands displayed intense CatB positivity. We conclude that CatB and CatS are often expressed together in neoplastic prostatic cells from pre‐invasive to invasive and clinically detectable stages, suggesting a putative role in local invasion, though other functions cannot be ruled out. © 2001 Wiley‐Liss, Inc.

[1]  I. Constable,et al.  The use of adenovirus-mediated gene transfer to develop a rat model for photoreceptor degeneration. , 2000, Investigative ophthalmology & visual science.

[2]  G. Dranoff,et al.  Cathepsin S required for normal MHC class II peptide loading and germinal center development. , 1999, Immunity.

[3]  W. Fiers,et al.  Atractyloside‐induced release of cathepsin B, a protease with caspase‐processing activity , 1998, FEBS letters.

[4]  S Hanash,et al.  A novel amplicon at 8p22-23 results in overexpression of cathepsin B in esophageal adenocarcinoma. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[5]  T. Watanabe,et al.  Participation of cathepsins B and D in apoptosis of PC12 cells following serum deprivation. , 1998, Biochemical and biophysical research communications.

[6]  Bonnie F. Sloane,et al.  Codistribution of procathepsin B and mature cathepsin B forms in human prostate tumors detected by confocal and immunofluorescence microscopy , 1998, The Anatomical record.

[7]  I. Constable,et al.  Expression of cathepsin S antisense transcripts by adenovirus in retinal pigment epithelial cells. , 1998, Investigative ophthalmology & visual science.

[8]  Ashraf Khan,et al.  Cathepsin B and tumor-associated laminin expression in the progression of colorectal adenoma to carcinoma. , 1998, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.

[9]  P. Libby,et al.  Expression of the elastolytic cathepsins S and K in human atheroma and regulation of their production in smooth muscle cells. , 1998, The Journal of clinical investigation.

[10]  R. Mitchell,et al.  Cathepsin S activity regulates antigen presentation and immunity. , 1998, The Journal of clinical investigation.

[11]  J. Kos,et al.  Cysteine proteinases in cancer progression and their clinical relevance for prognosis. , 1998, Biological chemistry.

[12]  Bonnie F. Sloane,et al.  Cathepsin B and human tumor progression. , 1998, Biological chemistry.

[13]  J. Kos,et al.  Cathepsins B, H, and L and their inhibitors stefin A and cystatin C in sera of melanoma patients. , 1997, Clinical cancer research : an official journal of the American Association for Cancer Research.

[14]  D. Buttle,et al.  Cathepsin B. , 1997, The international journal of biochemistry & cell biology.

[15]  H. Chapman,et al.  Emerging roles for cysteine proteases in human biology. , 1997, Annual review of physiology.

[16]  M. Žargi,et al.  Prognostic value of cathepsins B, H, L, D and their endogenous inhibitors stefins A and B in head and neck carcinoma. , 1996, Biological chemistry Hoppe-Seyler.

[17]  M. Duffy,et al.  Proteases as prognostic markers in cancer. , 1996, Clinical cancer research : an official journal of the American Association for Cancer Research.

[18]  H. Ploegh,et al.  Essential role for cathepsin S in MHC class II-associated invariant chain processing and peptide loading. , 1996, Immunity.

[19]  N. Rosenberg,et al.  Cathepsins D, B, and L in malignant human lung tissue. , 1996, Clinical cancer research : an official journal of the American Association for Cancer Research.

[20]  P. Canoll,et al.  Expression of Rat Cathepsin S in Phagocytic Cells (*) , 1996, The Journal of Biological Chemistry.

[21]  W. Grizzle,et al.  Biomarker expression in prostatic intraepithelial neoplasia. , 1996, European urology.

[22]  Bonnie F. Sloane,et al.  Cysteine proteases and tumor progression , 1995 .

[23]  J. Kos,et al.  Lysosomal proteases cathepsins D, B, H, L and their inhibitors stefins A and B in head and neck cancer. , 1995, Biological chemistry Hoppe-Seyler.

[24]  P. Dettmar,et al.  Prognostic value of the cysteine proteases cathepsins B and cathepsin L in human breast cancer. , 1995, Clinical cancer research : an official journal of the American Association for Cancer Research.

[25]  David G. Bostwick,et al.  High grade prostatic intraepithelial neoplasia. The most likely precursor of prostate cancer , 1995 .

[26]  D. Selkoe,et al.  The lysosomal cysteine protease, cathepsin S, is increased in Alzheimer's disease and Down syndrome brain. An immunocytochemical study. , 1995, The American journal of pathology.

[27]  Bonnie F. Sloane,et al.  Immunohistochemical localization of cathepsin B in neoplastic human prostate , 1995, The Prostate.

[28]  Bonnie F. Sloane,et al.  Cathepsin B in angiogenesis of human prostate: An immunohistochemical and immunoelectron microscopic analysis , 1995, The Anatomical record.

[29]  K. Wong,et al.  Cathepsin B, a cysteine protease implicated in metastatic progression, is also expressed during regression of the rat prostate and mammary glands. , 1994, European journal of biochemistry.

[30]  Bonnie F. Sloane,et al.  Immunohistochemical analysis of cathepsins D, B, and L in human breast cancer. , 1994, Human pathology.

[31]  Bonnie F. Sloane,et al.  Cathepsin B expression in colorectal carcinomas correlates with tumor progression and shortened patient survival. , 1994, The American journal of pathology.

[32]  A. C. Webb,et al.  Human cathepsin S: chromosomal localization, gene structure, and tissue distribution. , 1994, The Journal of biological chemistry.

[33]  M. Tötsch,et al.  Immunohistochemical localization of chromogranins A and B and secretogranin II in normal, hyperplastic and neoplastic prostate , 1994, Histopathology.

[34]  Tsuyoshi Watanabe,et al.  CELL AND TISSUE DISTRIBUTION OF LYSOSOMAL CYSTEINE PROTEINASES, CATHEPSINS B, H, AND L, AND THEIR BIOLOGICAL ROLES , 1994 .

[35]  R. Nagle,et al.  Adhesion molecules, extracellular matrix, and proteases in prostate carcinoma. , 1994, Journal of cellular biochemistry. Supplement.

[36]  Bonnie F. Sloane,et al.  Clinicopathologic significance of cathepsin B immunostaining in transitional neoplasia. , 1994, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.

[37]  Bonnie F. Sloane,et al.  Human tumour cathepsin B. Comparison with normal liver cathepsin B. , 1992, The Biochemical journal.

[38]  D. Rich,et al.  Molecular cloning and expression of human alveolar macrophage cathepsin S, an elastinolytic cysteine protease. , 1992, The Journal of biological chemistry.