Modern Optics, Electronics and High Precision Techniques in Cell Biology

The techniques described in this book provide an alternative method to understanding cellular biological structures and processes. Molecular genetic methods mainly analyze single molecules or very small cellular parts which do not necessarily enhance our understanding of the fundamental cellular processes. However, using techniques such as atomic force or infrared microscopy, neutron reflection, stopped-flow cytometry, laser microscopy or biocensoric-based electronics, it is possible to study cells in their entirety. Physiological processes of cells, such as movement, development, plasticity, regeneration and communication, can be visualized using the high precision biophysical techniques described here.

[1]  Kawasaki,et al.  Confined semiflexible polymer chains. , 1995, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.

[2]  C. Coppin,et al.  Quantitation of liquid-crystalline ordering in F-actin solutions. , 1992, Biophysical journal.

[3]  E. Sackmann,et al.  Specular reflection of neutrons at phospholipid monolayers. Changes of monolayer structure and headgroup hydration at the transition from the expanded to the condensed phase state. , 1990, Biophysical journal.

[4]  S. Prager,et al.  Dynamics of Entangled Polymer Liquids: Do Linear Chains Reptate? , 2007 .

[5]  T. Pollard,et al.  The structural basis for the intrinsic disorder of the actin filament: the "lateral slipping" model , 1991, The Journal of cell biology.

[6]  S. Edwards,et al.  The Theory of Polymer Dynamics , 1986 .

[7]  Toshio Yanagida,et al.  Sub-piconewton force fluctuations of actomyosin in vitro , 1991, Nature.

[8]  J. Magda,et al.  The transport properties of rod-like particles via molecular dynamics. I. Bulk fluid , 1986 .

[9]  M. Huggins.  Viscoelastic Properties of Polymers. , 1961 .

[10]  D E Smith,et al.  Direct observation of tube-like motion of a single polymer chain. , 1994, Science.

[11]  A. Noegel,et al.  The actin-binding protein hisactophilin binds in vitro to partially charged membranes and mediates actin coupling to membranes. , 1995, Biochemistry.

[12]  James H. Davis,et al.  Phase equilibria of cholesterol/dipalmitoylphosphatidylcholine mixtures: 2H nuclear magnetic resonance and differential scanning calorimetry. , 1990, Biochemistry.

[13]  S. Smith,et al.  Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads. , 1992, Science.

[14]  T. Russell,et al.  X-ray and neutron reflectivity for the investigation of polymers , 1990 .

[15]  H. Gaub,et al.  Viscoelastic moduli of sterically and chemically cross-linked actin networks in the dilute to semidilute regime: measurements by oscillating disk rheometer , 1991 .

[16]  J. Als-Nielsen,et al.  Recognition processes at a functionalized lipid surface observed with molecular resolution. , 1991, Biophysical journal.

[17]  E. H. Linfoot.  Principles of Optics , 1961 .

[18]  M. Carlier.  Dynamic actin , 1993, Current Biology.

[19]  Zhengxiu Chen.  Nematic ordering in semiflexible polymer chains , 1993 .

[20]  G. Gerisch,et al.  The pH-sensitive Actin-binding Protein Hisactophilin of Dictyostelium Exists in Two Isoforms Which Both Are Myristoylated and Distributed between Plasma Membrane and Cytoplasm (*) , 1995, The Journal of Biological Chemistry.

[21]  W. Goldmann,et al.  Talin anchors and nucleates actin filaments at lipid membranes A direct demonstration , 1992, FEBS letters.

[22]  B. Humbel,et al.  Hisactophilin, a histidine-rich actin-binding protein from Dictyostelium discoideum. , 1989, The Journal of biological chemistry.

[23]  Rosa María Velasco,et al.  Remarks on polyelectrolyte conformation , 1976 .

[24]  M. Schleicher,et al.  Hisactophilin-mediated binding of actin to lipid lamellae: a neutron reflectivity study of protein membrane coupling. , 1996, Biophysical journal.

[25]  W. Goldmann,et al.  Interaction of NBD‐talin with lipid monolayers , 1993, FEBS letters.

[26]  P. Janmey,et al.  Viscoelastic properties of vimentin compared with other filamentous biopolymer networks , 1991, The Journal of cell biology.

[27]  T. Holak,et al.  Structure of hisactophilin is similar to interleukin-1β and fibroblast growth factor , 1994, Nature.

[28]  P. Janmey,et al.  Cooperativity in F-actin: binding of gelsolin at the barbed end affects structure and dynamics of the whole filament. , 1996, Journal of molecular biology.

[29]  P. Janmey,et al.  Structure and mobility of actin filaments as measured by quasielastic light scattering, viscometry, and electron microscopy. , 1986, The Journal of biological chemistry.

[30]  K. Kjaer,et al.  Structural properties of phosphatidylcholine in a monolayer at the air/water interface: Neutron reflection study and reexamination of x-ray reflection measurements. , 1991, Biophysical journal.

[31]  J. Käs,et al.  Direct Measurement of the Wave-Vector-Dependent Bending Stiffness of Freely Flickering Actin Filaments , 1993 .

[32]  E. Elson,et al.  Cellular mechanics as an indicator of cytoskeletal structure and function. , 1988, Annual review of biophysics and biophysical chemistry.

[33]  R. Pecora,et al.  Reevaluation of the dynamic model for rotational diffusion of thin, rigid rods in semidilute solution , 1985 .

[34]  G. Vroege,et al.  Induced Chain Rigidity, Splay Modulus, and Other Properties of Nematic Polymer Liquid Crystals , 1988 .

[35]  P. Janmey,et al.  Effect of ATP on actin filament stiffness , 1990, Nature.

[36]  E. Sackmann,et al.  Coupling of spectrin and polylysine to phospholipid monolayers studied by specular reflection of neutrons. , 1991, Biophysical journal.

[37]  D H Wachsstock,et al.  Cross-linker dynamics determine the mechanical properties of actin gels. , 1994, Biophysical journal.

[38]  P. G. de Gennes,et al.  Kinetics of diffusion‐controlled processes in dense polymer systems. II. Effects of entanglements , 1982 .

[39]  J. Casella,et al.  Interaction of Cap Z with actin. The NH2-terminal domains of the alpha 1 and beta subunits are not required for actin capping, and alpha 1 beta and alpha 2 beta heterodimers bind differentially to actin. , 1994, The Journal of biological chemistry.

[40]  M. Fechheimer,et al.  Formation of liquid crystals from actin filaments. , 1993, Biochemistry.

[41]  P. Gennes.  Reptation of a Polymer Chain in the Presence of Fixed Obstacles , 1971 .

[42]  R Ezzell,et al.  F-actin, a model polymer for semiflexible chains in dilute, semidilute, and liquid crystalline solutions. , 1996, Biophysical journal.