Copper wire amperometric detector for capillary electrophoresis

The use of a copper wire amperometric detector for capillary electrophoresis is reported. Detection is accomplished by use of a porous glass joint, which allows amperometric detection at a copper wire electrode inserted in the end of the capillary. An anodic current is produced by a change in the copper oxide film solubility, resulting from complexation of copper ions with certain analytes at the electrode surface. This copper/copper oxide electrode has been used to detect nonelectroactive native amino acids and dipeptides. Subfemtomole detection limits in picoliter injection volumes have been obtained without solute derivatization. In addition, simultaneous analysis of nonelectroactive amino acids and electroactive catecholamines has been demonstrated.

[1]  R. W. Frei,et al.  Amperometric detection of amino acids with a passivated copper electrode , 1982 .

[2]  P. Haddad,et al.  Potentiometric flow-injection determination of copper complexing inorganic anions with a copper wire indicator electrode , 1984 .

[3]  A. Ewing,et al.  Dopamine concentration in the cytoplasmic compartment of single neurons determined by capillary electrophoresis , 1990, Journal of Neuroscience Methods.

[4]  K. Stulík,et al.  Analysis of dipeptides by reversed-phase high-performance liquid chromatography without derivatization using amperometric detection on a copper electrode. , 1988, Journal of chromatography.

[5]  K. Stulík,et al.  Amperometric flow detection with a copper working electrode-response mechanism and application to various compounds. , 1988, Talanta.

[6]  P. Haddad,et al.  Application of indirect potentiometric detection with a metallic copper electrode to ion chromatography of transition metal ions , 1985 .

[7]  P. Haddad,et al.  High-performance liquid chromatography of organic acids with potentiometric detection using a metallic copper electrode , 1984 .

[8]  R. W. Frei,et al.  Amperometric detection of amino acids in high-performance liquid chromatography with a copper electrode. , 1983, Journal of chromatography.

[9]  A. Ewing,et al.  Capillary zone electrophoresis with electrochemical detection in 12.7 microns diameter columns. , 1988, Analytical chemistry.

[10]  P. Haddad,et al.  Ion chromatography of inorganic anions with potentiometric detection using a metallic copper electrode , 1985 .

[11]  A. Ewing,et al.  Amperometric detection of catechols in capillary zone electrophoresis with normal and micellar solutions. , 1988, Analytical chemistry.

[12]  J. Jorgenson,et al.  Capillary zone electrophoresis. , 1983, Science.

[13]  M. Hitchman,et al.  Potentiometric monitoring of proteins. Part 3.—Direct potentiometry with a copper electrode , 1986 .

[14]  A. Ewing,et al.  Separation of serotonin from catechols by capillary zone electrophoresis with electrochemical detection. , 1989, Analytical chemistry.

[15]  K. Stulík,et al.  Operational parameters of voltammetric high-performance liquid chromatographic detectors with copper electrodes and application to a determination of some fodder biofactors. , 1986, Journal of chromatography.

[16]  R. W. Frei,et al.  Rapid determination of phenylalanine and tyrosine in urine and serum by HPLC with electrochemical detection. , 1983, Journal of pharmaceutical and biomedical analysis.

[17]  B. Karger,et al.  High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins. , 1987, Journal of chromatography.

[18]  A. Ewing,et al.  Capillary electrophoresis with indirect amperometric detection. , 1990, Journal of chromatography.

[19]  A. Ewing,et al.  Capillary electrophoresis in 2 and 5 microns diameter capillaries: application to cytoplasmic analysis. , 1990, Analytical chemistry.

[20]  N. Dovichi,et al.  Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence. , 1988, Science.