Cell/Material Interfaces: Influence of Surface Chemistry and Surface Topography on Cell Adhesion

The need to control the adhesion of cells to material surfaces plays an important role in determining the design of biomaterial substrates for biotechnology and tissue-engineering applications. As a the first step in a cascade of cellular events, adhesion affects many aspects of cell function, including spreading, migration, proliferation and differentiation. After a short description of cell adhesion and essential molecules involved in, the present knowledge on the influence of surface topography on cell behavior will be described by considering not only the amplitude of the surface topography but also its organization at all scales (micro- and nano-scale). The biological mechanisms underlying the cell response to topography will be evoked. Secondly, the influence of surface chemistry as well as surface energy on cell adhesion will be described. Thirdly, as the cells never interact with a bare material but with materials on which the proteins from biological fluids have adsorbed, some studies on the role of proteins in cell adhesion will be used to illustrate this point. Finally, the influence of substrate mechanics on cell differentiation will be described.

[1]  P. Janmey,et al.  Tissue Cells Feel and Respond to the Stiffness of Their Substrate , 2005, Science.

[2]  Susan C Barnett,et al.  Long-term neurite orientation on astrocyte monolayers aligned by microtopography. , 2007, Biomaterials.

[3]  W. Soboyejo,et al.  Cell/surface interactions and adhesion on Ti-6Al-4V: effects of surface texture. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[4]  David Boettiger,et al.  Effect of biomaterial surface properties on fibronectin–α5β1 integrin interaction and cellular attachment , 2006 .

[5]  J. Ricci,et al.  Connective-tissue responses to defined biomaterial surfaces. II. Behavior of rat and mouse fibroblasts cultured on microgrooved substrates. , 2008, Journal of biomedical materials research. Part A.

[6]  D. Brunette,et al.  The effects of the surface topography of micromachined titanium substrata on cell behavior in vitro and in vivo. , 1999, Journal of biomechanical engineering.

[7]  Patricia J Keely,et al.  Focal adhesion regulation of cell behavior. , 2004, Biochimica et biophysica acta.

[8]  R E Baier,et al.  Surface properties determine bioadhesive outcomes: methods and results. , 1984, Journal of biomedical materials research.

[9]  Christopher S. Chen,et al.  Cells lying on a bed of microneedles: An approach to isolate mechanical force , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[10]  M. Textor,et al.  single cells{ , 2007 .

[11]  D. Landolt,et al.  Variable length scale analysis of surface topography: characterization of titanium surfaces for biomedical applications , 1998 .

[12]  Cameron J Wilson,et al.  Mediation of biomaterial-cell interactions by adsorbed proteins: a review. , 2005, Tissue engineering.

[13]  A. Abbott.  Cell culture: Biology's new dimension , 2003, Nature.

[14]  Maxence Bigerelle,et al.  Modelling approach in cell/material interactions studies. , 2006, Biomaterials.

[15]  C. Oakley,et al.  The sequence of alignment of microtubules, focal contacts and actin filaments in fibroblasts spreading on smooth and grooved titanium substrata. , 1993, Journal of cell science.

[16]  M. Yoshinari,et al.  Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[17]  J. Jansen,et al.  The effect of combined cyclic mechanical stretching and microgrooved surface topography on the behavior of fibroblasts. , 2005, Journal of biomedical materials research. Part A.

[18]  M. Textor,et al.  Surface engineering approaches to micropattern surfaces for cell-based assays. , 2006, Biomaterials.

[19]  K. Nguyen,et al.  Nanotopography: cellular responses to nanostructured materials. , 2006, Journal of nanoscience and nanotechnology.

[20]  P. Tengvall,et al.  In Vitro Plasma Protein Adsorption on ω-Functionalized Alkanethiolate Self-Assembled Monolayers , 1997 .

[21]  J. Jansen,et al.  Integrins as linker proteins between osteoblasts and bone replacing materials. A critical review. , 2005, Biomaterials.

[22]  Manuel Théry,et al.  Cell distribution of stress fibres in response to the geometry of the adhesive environment. , 2006, Cell motility and the cytoskeleton.

[23]  P Ducheyne,et al.  Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. , 1999, Biomaterials.

[24]  U. Joos,et al.  Basic reactions of osteoblasts on structured material surfaces. , 2005, European cells & materials.

[25]  D. Ingber,et al.  Cellular mechanotransduction: putting all the pieces together again , 2006, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[26]  Benjamin G. Keselowsky,et al.  Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[27]  Andrés J. García.  Get a grip: integrins in cell-biomaterial interactions. , 2005, Biomaterials.

[28]  D. Landolt,et al.  Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography. , 2004, Biomaterials.

[29]  S. Itzkovitz,et al.  Functional atlas of the integrin adhesome , 2007, Nature Cell Biology.

[30]  Pedro Madureira,et al.  The influence of functional groups of self-assembled monolayers on fibrous capsule formation and cell recruitment. , 2006, Journal of biomedical materials research. Part A.

[31]  C. Lohmann,et al.  Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. , 1998, Biomaterials.

[32]  Adam W Feinberg,et al.  Systematic variation of microtopography, surface chemistry and elastic modulus and the state dependent effect on endothelial cell alignment. , 2008, Journal of biomedical materials research. Part A.

[33]  Xiaolong Zhu,et al.  Effects of topography and composition of titanium surface oxides on osteoblast responses. , 2004, Biomaterials.

[34]  J. Planell,et al.  Transparent micro- and nanopatterned poly(lactic acid) for biomedical applications. , 2006, Journal of biomedical materials research. Part A.

[35]  Julie Gold,et al.  Quantitative assessment of the response of primary derived human osteoblasts and macrophages to a range of nanotopography surfaces in a single culture model in vitro. , 2003, Biomaterials.

[36]  Benjamin Geiger,et al.  Adhesion-mediated mechanosensitivity: a time to experiment, and a time to theorize. , 2006, Current opinion in cell biology.

[37]  Lee,et al.  The Effect of Fluid Shear Stress on Endothelial Cell Adhesiveness to Polymer Surfaces with Wettability Gradient. , 2000, Journal of colloid and interface science.

[38]  J. Jansen,et al.  Early spreading events of fibroblasts on microgrooved substrates. , 2000, Journal of biomedical materials research.

[39]  Matthew J Dalby,et al.  Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands. , 2002, Experimental cell research.

[40]  M. Alexander,et al.  Investigation of cell-surface interactions using chemical gradients formed from plasma polymers. , 2008, Biomaterials.

[41]  Lifeng Chi,et al.  Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir-Blodgett lithography. , 2005, Biomaterials.

[42]  H. Busscher,et al.  Amount and surface structure of albumin adsorbed to solid substrata with different wettabilities in a parallel plate flow cell. , 1990, Journal of biomedical materials research.

[43]  F. Goubard,et al.  Kinetics of conformational changes of fibronectin adsorbed onto model surfaces. , 2008, Colloids and surfaces. B, Biointerfaces.

[44]  B. Geiger,et al.  Encounters in Space , 2001, Science.

[45]  Maxence Bigerelle,et al.  Topography effects of pure titanium substrates on human osteoblast long-term adhesion. , 2005, Acta biomaterialia.

[46]  Matthew John Dalby,et al.  Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography. , 2004, Biomaterials.

[47]  V. Hasırcı,et al.  Microstructured Surfaces Cause Severe but Non‐Detrimental Deformation of the Cell Nucleus , 2009 .

[48]  M. Biggs,et al.  Adhesion formation of primary human osteoblasts and the functional response of mesenchymal stem cells to 330 nm deep microgrooves , 2008, Journal of The Royal Society Interface.

[49]  Douglas W DeSimone,et al.  Cell adhesion receptors in mechanotransduction. , 2008, Current opinion in cell biology.

[50]  Maxence Bigerelle,et al.  Statistical demonstration of the relative effect of surface chemistry and roughness on human osteoblast short-term adhesion , 2006, Journal of materials science. Materials in medicine.

[51]  Maxence Bigerelle,et al.  Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses. , 2000, Journal of biomedical materials research.

[52]  A. Donald,et al.  Identification of three regimes of behavior for cell attachment on topographically patterned substrates. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[53]  J. Jacobs,et al.  Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion. , 2001, Tissue engineering.

[54]  B D Boyan,et al.  Role of material surfaces in regulating bone and cartilage cell response. , 1996, Biomaterials.

[55]  J. Murphy-Ullrich.  The de-adhesive activity of matricellular proteins: is intermediate cell adhesion an adaptive state? , 2001, The Journal of clinical investigation.

[56]  C. Ober,et al.  Self-assembled monolayers and polymer brushes in biotechnology: current applications and future perspectives. , 2005, Biomacromolecules.

[57]  Newell R Washburn,et al.  Combinatorial screen of the effect of surface energy on fibronectin-mediated osteoblast adhesion, spreading and proliferation. , 2006, Biomaterials.

[58]  Sandra Downes,et al.  Protein adsorption and human osteoblast-like cell attachment and growth on alkylthiol on gold self-assembled monolayers. , 2002, Journal of biomedical materials research.

[59]  C. Werner,et al.  Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies. , 2004, Biomaterials.

[60]  G. Gronowicz,et al.  Integrin-mediated signaling in osteoblasts on titanium implant materials. , 2000, Journal of biomedical materials research.

[61]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[62]  Maxence Bigerelle,et al.  Improvement in the morphology of Ti-based surfaces: a new process to increase in vitro human osteoblast response. , 2002, Biomaterials.

[63]  L F Cooper,et al.  A role for surface topography in creating and maintaining bone at titanium endosseous implants. , 2000, The Journal of prosthetic dentistry.

[64]  K. Athanasiou,et al.  Extracellular matrix cell adhesion peptides: functional applications in orthopedic materials. , 2000, Tissue engineering.

[65]  C. Tickle,et al.  Differences in alignment of normal and transformed cells on glass fibres. , 1981, Experimental cell research.

[66]  Maxence Bigerelle,et al.  Relevance of roughness parameters for describing and modelling machined surfaces , 2003 .

[67]  G. Gronowicz,et al.  Integrin-mediated signaling regulates AP-1 transcription factors and proliferation in osteoblasts. , 2000, Journal of biomedical materials research.

[68]  Sang Jin Lee,et al.  The effect of surface wettability on induction and growth of neurites from the PC-12 cell on a polymer surface. , 2003, Journal of colloid and interface science.

[69]  David Farrar,et al.  Interpretation of protein adsorption: surface-induced conformational changes. , 2005, Journal of the American Chemical Society.

[70]  A Curtis,et al.  Topographical control of cells. , 1997, Biomaterials.

[71]  A. Curtis,et al.  The influence of microscale topography on fibroblast attachment and motility. , 2004, Biomaterials.

[72]  H. Busscher,et al.  The influence of substratum surface free energy on growth and spreading of human fibroblasts in the presence and absence of serum proteins. , 1986, Journal of biomedical materials research.

[73]  M. Diniz,et al.  Surface topography modulates the osteogenesis in human bone marrow cell cultures grown on titanium samples prepared by a combination of mechanical and acid treatments , 2002, Journal of materials science. Materials in medicine.

[74]  Claude Martelet,et al.  Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour , 2003 .

[75]  B. Boyan,et al.  Titanium surface roughness alters responsiveness of MG63 osteoblast‐like cells to 1α,25‐(OH)2D3 , 1998 .

[76]  Y. Wang,et al.  Cell locomotion and focal adhesions are regulated by substrate flexibility. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[77]  Martin Schuler,et al.  Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients. , 2007, Biomaterials.

[78]  A S G Curtis,et al.  Morphological and microarray analysis of human fibroblasts cultured on nanocolumns produced by colloidal lithography. , 2005, European cells & materials.

[79]  J. Heath,et al.  A new hypothesis of contact guidance in tissue cells. , 1976, Experimental cell research.

[80]  J. Jansen,et al.  Growth behavior of fibroblasts on microgrooved polystyrene. , 1998, Biomaterials.

[81]  M. Titus,et al.  Myosins: matching functions with motors. , 1998, Current opinion in cell biology.

[82]  Besim Ben Nissan,et al.  The effect of surface chemistry modification of titanium alloy on signalling pathways in human osteoblasts. , 2005, Biomaterials.

[83]  P Bongrand,et al.  Cell fitting to adhesive surfaces: A prerequisite to firm attachment and subsequent events. , 2002, European cells & materials.

[84]  Andrés J. García,et al.  Stick and grip , 2007, Cell Biochemistry and Biophysics.

[85]  Effect of a gold–palladium coating on the long-term adhesion of human osteoblasts on biocompatible metallic materials , 2006 .

[86]  W. Su,et al.  The geometric pattern of a pillared substrate influences the cell-process distribution and shapes of fibroblasts. , 2006, Micron.

[87]  Maxence Bigerelle,et al.  The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour. , 2000, Biomaterials.

[88]  Kenneth M. Yamada,et al.  Cell interactions with three-dimensional matrices. , 2002, Current opinion in cell biology.

[89]  B. Ratner,et al.  Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers. , 2006, Biomaterials.

[90]  G. Truskey,et al.  Effect of the conformation and orientation of adsorbed fibronectin on endothelial cell spreading and the strength of adhesion. , 1993, Journal of biomedical materials research.

[91]  C. Wilkinson,et al.  The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. , 2007, Nature materials.

[92]  D. Discher,et al.  Cell differentiation through tissue elasticity-coupled, myosin-driven remodeling. , 2008, Current opinion in cell biology.

[93]  K. Nguyen,et al.  Cellular and molecular responses of smooth muscle cells to surface nanotopography. , 2007, Journal of nanoscience and nanotechnology.

[94]  E. Sairenji,et al.  Effects of "wettability" of biomaterials on culture cells. , 1989, The Journal of oral implantology.

[95]  S. Dedhar,et al.  Bi-directional signal transduction by integrin receptors. , 2000, The international journal of biochemistry & cell biology.

[96]  A S G Curtis,et al.  Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano‐topography and fibroblast filopodia , 2004, Cell biology international.

[97]  J. Lausmaa,et al.  Surface characterization and biological evaluation of spark-eroded surfaces , 1997, Journal of materials science. Materials in medicine.

[98]  Ali Othmane,et al.  Effect of surface topography and chemistry on adhesion, orientation and growth of fibroblasts on nickel–titanium substrates , 2002 .

[99]  K. Leong,et al.  Significance of synthetic nanostructures in dictating cellular response. , 2005, Nanomedicine : nanotechnology, biology, and medicine.

[100]  Kenneth M. Yamada,et al.  Cell biology: Survival in three dimensions , 2002, Nature.

[101]  P. Campbell,et al.  Variation in surface texture measurements. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[102]  P. Bongrand,et al.  Is there a predictable relationship between surface physical-chemical properties and cell behaviour at the interface? , 2004, European cells & materials.

[103]  Maxence Bigerelle,et al.  Statistical correlation between cell adhesion and proliferation on biocompatible metallic materials. , 2005, Journal of biomedical materials research. Part A.

[104]  M. Dembo,et al.  Cell movement is guided by the rigidity of the substrate. , 2000, Biophysical journal.

[105]  J. H. Lee,et al.  Platelet adhesion onto chargeable functional group gradient surfaces. , 1998, Journal of biomedical materials research.

[106]  Jeffrey J. Fredberg,et al.  Universality in cell mechanics , 2008 .

[107]  John T Elliott,et al.  Gradient substrate assembly for quantifying cellular response to biomaterials. , 2006, Journal of biomedical materials research. Part A.

[108]  E. Vogler,et al.  Systematic variation in osteoblast adhesion and phenotype with substratum surface characteristics. , 2004, Journal of biomedical materials research. Part A.

[109]  J. Jansen,et al.  Attachment of fibroblasts on smooth and microgrooved polystyrene. , 1999, Journal of biomedical materials research.

[110]  Pierre Hardouin,et al.  Quantitative kinetic analysis of gene expression during human osteoblastic adhesion on orthopaedic materials. , 2006, Biomaterials.

[111]  Richard O Hynes,et al.  Integrins Bidirectional, Allosteric Signaling Machines , 2002, Cell.

[112]  K. Anselme,et al.  Osteoblast adhesion on biomaterials. , 2000, Biomaterials.

[113]  B. Boyan,et al.  Surface roughness modulates the response of MG63 osteoblast-like cells to 1,25-(OH)(2)D(3) through regulation of phospholipase A(2) activity and activation of protein kinase A. , 1999, Journal of biomedical materials research.

[114]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.