Poly(ethylene‐co‐vinyl alcohol) Membranes with Specific Adsorption Properties for Potential Clinical Application

Abstract The preparation of novel polymeric systems through Molecular Imprinting Technology (MIT) for potential application in extracorporeal blood purification is described. Membranes based on poly(ethylene‐co‐vinyl alcohol) material, produced through a phase inversion method, were modified introducing in their structure specific binding sites for lipid and/or protein molecules. Membranes prepared are intended to selectively remove low density lipoproteins and cholesterol (LDL) from the plasma, by using interactions at a molecular level, between the molecularly imprinted membrane and specific target molecules, created during the preparation procedure. The binding performances of membranes and their potentiality as adsorbents for two different model target compounds, a phospholipid (phosphotidylcholine, PC) and a protein (α‐amylase enzyme) were investigated, showing improved adsorption capacity with respect to unmodified control membranes. In addition, molecularly imprinted poly(ethylene‐co‐vinyl alcohol) materials in the shape of microparticles, using the same templates, were prepared and studied for their potential use as adsorbers into a column.

[1]  M. Kulkarni,et al.  Enhanced capacities and selectivities for cholesterol in aqueous media by molecular imprinting: role of novel cross-linkers. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[2]  M. Komiyama,et al.  Molecularly Imprinted Cyclodextrins as Selective Receptors for Steroids1 , 1999 .

[3]  M. Ulbricht.  Membrane separations using molecularly imprinted polymers. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[4]  G. Wulff.  Molecular Imprinting in Cross‐Linked Materials with the Aid of Molecular Templates— A Way towards Artificial Antibodies , 1995 .

[5]  M. Drew,et al.  Synthesis and evaluation of a solid supported molecular tweezer type receptor for cholesterol , 2003 .

[6]  Takaomi Kobayashi,et al.  Molecular Imprint Membranes Prepared by the Phase Inversion Precipitation Technique. 2. Influence of Coagulation Temperature in the Phase Inversion Process on the Encoding in Polymeric Membranes , 1997 .

[7]  G Ciardelli,et al.  Molecularly imprinted bioartificial membranes for the selective recognition of biological molecules. Part 2: release of components and thermal analysis , 2005, Journal of biomaterials science. Polymer edition.

[8]  K. Sreenivasan.  Synthesis and evaluation of a beta cyclodextrin-based molecularly imprinted copolymer , 1998 .

[9]  G. Ciardelli,et al.  Polymeric devices containing imprinted nanospheres: a novel approach to improve recognition in water for clinical uses , 2005 .

[10]  G. Ciardelli,et al.  Molecularly imprinted bioartificial membranes for the selective recognition of biological molecules , 2004, Journal of biomaterials science. Polymer edition.

[11]  Hiroyuki Asanuma,et al.  Tailor‐Made Receptors by Molecular Imprinting , 2000 .

[12]  B. Sellergren,et al.  Imprinted polymers for selective adsorption of cholesterol from gastrointestinal fluids , 1998 .

[13]  Takaomi Kobayashi,et al.  Molecular imprint membranes prepared by the phase inversion precipitation technique , 1996 .

[14]  K Mosbach,et al.  Molecular Imprinting , 1994, Trends in biochemical sciences.

[15]  A. Denizli.  Preparation of immuno-affinity membranes for cholesterol removal from human plasma. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[16]  S. Moorjani,et al.  Removal of Cholesterol from Blood by Affinity Binding to Heparin-Agarose: Evaluation on Treatment in Homozygous Familial Hypercholesterolemia , 1980, Pediatric Research.

[17]  E. Vulfson,et al.  Surface imprinting of cholesterol on Submicrometer core-shell emulsion particles , 2001 .

[18]  M. Whitcombe,et al.  Molecularly imprinted nanoparticles prepared by core‐shell emulsion polymerization , 2000 .

[19]  G. Ciardelli,et al.  Supported imprinted nanospheres for the selective recognition of cholesterol. , 2006, Biosensors & bioelectronics.

[20]  S. Yokoyama,et al.  Selective Removal of Low Density Lipoprotein by Plasmapheresis in Familial Hypercholesterolemia , 1985, Arteriosclerosis.

[21]  Ian A. Nicholls,et al.  Receptor and transport properties of imprinted polymer membranes – a review , 1999 .

[22]  V. Armstrong,et al.  The HELP‐LDL‐apheresis multicentre study, an angiographically assessed trial on the role of LDL‐apheresis in the secondary prevention of coronary heart disease , 1991, European journal of clinical investigation.

[23]  Michael J. Whitcombe,et al.  A NEW METHOD FOR THE INTRODUCTION OF RECOGNITION SITE FUNCTIONALITY INTO POLYMERS PREPARED BY MOLECULAR IMPRINTING : SYNTHESIS AND CHARACTERIZATION OF POLYMERIC RECEPTORS FOR CHOLESTEROL , 1995 .

[24]  T. Takeuchi,et al.  Preparation of sterol-imprinted polymers with the use of 2-(methacryloyloxy)ethyl phosphate. , 2001, Journal of chromatography. A.