DNA amplification for detection of the XV-2c polymorphism linked to cystic fibrosis.

OTHER COMMENTS: Oligonucleotide sequences for amplification are as follows: 5'-GTTGAAGTGAATTGAATG-3' and 5'-GTTCAAACTATGTCAAAG-3'. DNA was amplified with Taq polymerase as recommended by the supplier, Cetus Corporation. The figure demonstrates the RFLP after amplification, enzyme digestion and direct visualization with ethidium bromide. M markers, U uncut with TaqI, C = cut with TaqI, and B = blank with no genomic DNA. Genotypes are indicated as (+) for presence of the TaqI site and (-) for absence.