Biochemical studies on muscaranic receptors in the salamander olfactory epithelium

Muscarinic cholinergic receptors in the olfactory epithelium of the salamander, Ambystoma tigrinum, were studied via binding of 3‐[3H]quinuclidinyl benzilate. The receptors are present on the olfactory receptor cells in the epithelium to an amount of 0.08 pmol/mg homogenate protein. Both choline acetyltransferase and acetylcholine esterase are present in the salamander olfactory epithelium.

[1]  F. Fonnum A radiochemical method for the estimation of choline acetyltransferase. , 1966, The Biochemical journal.

[2]  J. Hirsch,et al.  Cell suspensions from rat olfactory neuroepithelium: Biochemical and histochemical characterization , 1979, Brain Research.

[3]  J. Hirsch,et al.  15 – Isolation, Separation, and Analysis of Cells from Olfactory Epithelium , 1981 .

[4]  Intracellular recordings from two cell types in an in vitro preparation of the salamander olfactory epithelium , 1983, Neuroscience Letters.

[5]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[6]  T. Getchell,et al.  Physiological activity of newly differentiated olfactory receptor neurons correlated with morphological recovery from olfactory nerve section in the salamander. , 1981, Journal of neurophysiology.

[7]  S. Takagi,et al.  Secretion and electrogenesis of the supporting cell in the olfactory epithelium , 1974, The Journal of physiology.

[8]  K. Courtney,et al.  A new and rapid colorimetric determination of acetylcholinesterase activity. , 1961, Biochemical pharmacology.

[9]  Irwin J. Kopin,et al.  The Biochemical Basis of Neuropharmacology , 1971, The Yale Journal of Biology and Medicine.

[10]  B. G. Wallace Distribution of AChE in cholinergic and non-cholinergic neurons , 1981, Brain Research.

[11]  F. Margolis,et al.  Denervation in the primary olfactory pathway of mice. III. Effect on enzymes of carnosine metabolism , 1976, Brain Research.

[12]  T. Getchell Analysis of intracellular recordings from salamander olfactory epithelium , 1977, Brain Research.