论文信息 - Rapid Detection of Ophiostoma piceae andO. quercus in Stained Wood by PCR

Rapid Detection of Ophiostoma piceae andO. quercus in Stained Wood by PCR

ABSTRACT A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.

[1] Thomas J. White,et al. PCR protocols: a guide to methods and applications. , 1990 .

[2] T. White. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[3] C. Brasier,et al. Sibling species within Ophiostoma piceae , 1993 .

[4] E. Halmschlager,et al. Differentiation of Ophiostoma piceae and Ophiostoma quercus by Morphology and RAPD Analysis , 1995 .

[5] K. Seifert. Sapstain of commercial lumber by species of Ophiostoma and Ceratocystis , 1993 .

[6] T. Bruns,et al. ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rusts , 1993, Molecular ecology.

[7] J. Henson,et al. Use of polymerase chain reaction to detect Gaeumannomyces graminis DNA in plants grown in artificially and naturally infested soil. , 1993 .

[8] H. Förster,et al. Molecular taxonomy of Phytophthora megasperma based on mitochondrial and nuclear DNA polymorphisms , 1993 .

[9] Jason M. Meyer,et al. Colonization of Oak Wilt Fungal Mats by Ophiostoma piceae During Spring in Minnesota. , 1997, Plant disease.

[10] T. Bruns,et al. Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots , 1992, Applied and environmental microbiology.