Behavior, Histopathology and Physiological Responses of Rat Fed Diets Containing Growth Hormone Transgenic Fish Meal

  The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.

[1]  L. Sundström,et al.  Assessing Ecological and Evolutionary Consequences of Growth-Accelerated Genetically Engineered Fishes , 2015 .

[2]  Zuoyan Zhu,et al.  Transgenic Common Carp Do Not Have the Ability to Expand Populations , 2013, PloS one.

[3]  A. Alimuddin,et al.  IDENTIFICATION OF MAJALAYA COMMON CARP STRAINS RESISTANT TO KHV INFECTION USING CYCA-DAB1*05 ALLELE AS THE MARKER , 2011 .

[4]  Zuoyan Zhu,et al.  Increased mortality of growth‐enhanced transgenic common carp (Cyprinus carpio L.) under short‐term predation risk , 2010 .

[5]  B. Knols,et al.  Defining environmental risk assessment criteria for genetically modified fishes to be placed on the EU market , 2010 .

[6]  E. Pothos,et al.  Deficits of mesolimbic dopamine neurotransmission in rat dietary obesity , 2009, Neuroscience.

[7]  Alimuddin,et al.  Transgenic Nile tilapia (Oreochromis niloticus) over-expressing growth hormone show reduced ammonia excretion , 2007 .

[8]  A. Armario.  The hypothalamic-pituitary-adrenal axis: what can it tell us about stressors? , 2006, CNS & neurological disorders drug targets.

[9]  H. Zbikowska,et al.  Fish can be First – Advances in Fish Transgenesis for Commercial Applications , 2003, Transgenic Research.

[10]  W. Muir,et al.  Possible ecological risks of transgenic organism release when transgenes affect mating success: sexual selection and the Trojan gene hypothesis. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Ann E. Kelley,et al.  GABA in the Nucleus Accumbens Shell Participates in the Central Regulation of Feeding Behavior , 1997, The Journal of Neuroscience.

[12]  Carlo A Biagi,et al.  Extraordinary salmon growth , 1994, Nature.

[13]  A. Morales,et al.  Safety Evaluation of Transgenic Tilapia with Accelerated Growth , 2017, Marine Biotechnology.

[14]  Balai Besar,et al.  KADAR HISTAMIN DAN TESTOSTERON, RESPONS FISIOLOGIS DAN HISTOLOGIS IKAN KARNIVORA DIBERI PAKAN BERUPA DAGING IKAN TRANSGENIK (Histamine and Testosterone Levels, Physiological and Histological Response of Carnivorous Fish Fed on Transgenic Fish Flesh) , 2013 .

[15]  Matthew Chang,et al.  Social Learning , 2021, Encyclopedia of Autism Spectrum Disorders.