Adaptive Histogram Thresholding-Based Leukocyte Image Segmentation

To improve the accuracy of leukocyte segmentation, this paper presents a novel method based on adaptive histogram thresholding (AHT). The proposed method first employs color component combination and AHT to extract the nucleus of leukocyte and utilizes image color features to remove the complex backgrounds such as red blood cells (RBCs) and substantial dyeing impurities. Then, Canny edge detection is performed to extract the entire leukocyte. Finally, the cytoplasm of the leukocyte is obtained by subtracting the nucleus with the entire leukocyte. Experimental results on an image dataset containing 60 leukocyte images show that the proposed method generates more accurate segmentation results than the counterparts.

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