Pulsed Magneto-motive Ultrasound Imaging Using Ultrasmall Magnetic Nanoprobes

Nano-sized particles are widely regarded as a tool to study biologic events at the cellular and molecular levels. However, only some imaging modalities can visualize interaction between nanoparticles and living cells. We present a new technique, pulsed magnetomotive ultrasound imaging, which is capable of in vivo imaging of magnetic nanoparticles in real time and at sufficient depth. In pulsed magneto-motive ultrasound imaging, an external high-strength pulsed magnetic field is applied to induce the motion within the magnetically labeled tissue and ultrasound is used to detect the induced internal tissue motion. Our experiments demonstrated a sufficient contrast between normal and iron-laden cells labeled with ultrasmall magnetic nanoparticles. Therefore, pulsed magnetomotive ultrasound imaging could become an imaging tool capable of detecting magnetic nanoparticles and characterizing the cellular and molecular composition of deep-lying structures.

[1]  J. Schenck.  Safety of Strong, Static Magnetic Fields , 2000, Journal of magnetic resonance imaging : JMRI.

[2]  M E Phelps,et al.  Positron emission tomography provides molecular imaging of biological processes. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[3]  J. Schenck.  The role of magnetic susceptibility in magnetic resonance imaging: MRI magnetic compatibility of the first and second kinds. , 1996, Medical physics.

[4]  R Weissleder,et al.  High-efficiency intracellular magnetic labeling with novel superparamagnetic-Tat peptide conjugates. , 1999, Bioconjugate chemistry.

[5]  M. Brechbiel,et al.  Specific targeting of folate–dendrimer MRI contrast agents to the high affinity folate receptor expressed in ovarian tumor xenografts , 2001, Magnetic Resonance Materials in Physics, Biology and Medicine.

[6]  K Mosbach,et al.  Preparation and application of magnetic polymers for targeting of drugs , 1979, FEBS letters.

[7]  Dwight G Nishimura,et al.  FeCo/graphitic-shell nanocrystals as advanced magnetic-resonance-imaging and near-infrared agents , 2006, Nature materials.

[8]  M.A. Lubinski,et al.  An elasticity microscope. Part I: Methods , 1997, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[9]  K. Widder,et al.  Magnetic guidance of drug‐carrying microspheres , 1978 .

[10]  Ralph Weissleder,et al.  Differential conjugation of tat peptide to superparamagnetic nanoparticles and its effect on cellular uptake. , 2002, Bioconjugate chemistry.

[11]  Werner Jaschke,et al.  Molecular imaging with nanoparticles: giant roles for dwarf actors , 2008, Histochemistry and Cell Biology.

[12]  S. Park,et al.  8B-2 Imaging of Iron Oxide Nanoparticles Using Magneto-Motive Ultrasound , 2007, 2007 IEEE Ultrasonics Symposium Proceedings.

[13]  M. Yacamán,et al.  Investigations on magnetic properties and structure for carbon encapsulated nanoparticles of Fe, Co, Ni , 2000 .

[14]  S. Emelianov,et al.  Detection of magnetic nanoparticles in tissue using magneto-motive ultrasound , 2006, Nanotechnology.

[15]  Dar-Ren Chen,et al.  Evaluation of image compression for computer-aided diagnosis of breast tumors in 3D sonography , 2006, SPIE Medical Imaging.

[16]  Q. Pankhurst,et al.  Applications of magnetic nanoparticles in biomedicine , 2003 .

[17]  Ronald G Blasberg,et al.  Molecular-genetic imaging: current and future perspectives. , 2003, The Journal of clinical investigation.

[18]  F. Foster,et al.  Frequency dependence of ultrasound attenuation and backscatter in breast tissue. , 1986, Ultrasound in medicine & biology.

[19]  D. McPherson,et al.  In vivo targeting of acoustically reflective liposomes for intravascular and transvascular ultrasonic enhancement. , 1999, Journal of the American College of Cardiology.

[20]  Kort Travis,et al.  Plasmon resonance coupling of metal nanoparticles for molecular imaging of carcinogenesis in vivo. , 2007, Journal of biomedical optics.

[21]  I. Nagano,et al.  Pulp ablation therapy by inductive heating: heat generation characteristics in the pulp cavity. , 2007, Oral diseases.

[22]  K. Widder,et al.  Magnetic Microspheres: A Model System for Site Specific Drug Delivery in Vivo 1 , 1978, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[23]  C. Nahmias.  Molecular imaging: the next frontier. , 2002, Canadian Association of Radiologists journal = Journal l'Association canadienne des radiologistes.

[24]  M.A. Lubinski,et al.  Speckle tracking methods for ultrasonic elasticity imaging using short-time correlation , 1999, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[25]  J. Dobson.  Magnetic nanoparticles for drug delivery , 2006 .

[26]  Robert N Grass,et al.  Covalently functionalized cobalt nanoparticles as a platform for magnetic separations in organic synthesis. , 2007, Angewandte Chemie.

[27]  N. Peppas,et al.  Diffusional characteristics of freeze/thawed poly(vinyl alcohol) hydrogels: applications to protein controlled release from multilaminate devices. , 2000, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[28]  S. Neubauer,et al.  Assessment of motion gating strategies for mouse magnetic resonance at high magnetic fields , 2004, Journal of magnetic resonance imaging : JMRI.

[29]  S. Cherry,et al.  Cardiac PET imaging in mice with simultaneous cardiac and respiratory gating , 2005, Physics in medicine and biology.

[30]  Jianping Wang,et al.  Fabrication of Core-shell Type FeCo-Au (Ag) High Moment Magnetic Nanoparticles , 2005 .

[31]  Suhyun Park,et al.  Functional and morphological ultrasonic biomicroscopy for tissue engineers , 2006, SPIE Medical Imaging.

[32]  R. Costo,et al.  The preparation of magnetic nanoparticles for applications in biomedicine , 2003 .

[33]  Michel Claudon,et al.  Ultrasound contrast agents: properties, principles of action, tolerance, and artifacts , 2001, European Radiology.

[34]  R Weissleder,et al.  Molecular imaging. , 2009, Radiology.

[35]  Alexander Petrovsky,et al.  Magnetic resonance imaging of inducible E-selectin expression in human endothelial cell culture. , 2002, Bioconjugate chemistry.

[36]  Jianping Wang,et al.  High-magnetic-moment core-shell-type FeCo-Au /Ag nanoparticles , 2005 .

[37]  M. Bandstra,et al.  Measurements of Fukushima fallout by the Berkeley Radiological Air and Water Monitoring project , 2011, 2011 IEEE Nuclear Science Symposium Conference Record.