The Amomum tsao-ko Essential Oils Inhibited Inflammation and Apoptosis through p38/JNK MAPK Signaling Pathway and Alleviated Gentamicin-Induced Acute Kidney Injury

The clinical application of gentamicin may lead to acute kidney injury (AKI), and the nephrotoxicity of gentamicin is related to the pathological mechanism of several oxidative and inflammatory cytokines. Plant-derived essential oils have good anti-inflammatory and antioxidant properties. This study aimed to clarify the protective effect of Amomum tsao-ko essential oils (AOs) on gentamicin-induced AKI in rats and its possible mechanism. The rat AKI model was induced by intraperitoneal injection of gentamicin. After 14 days of oral AO treatment, the renal function and pathological changes of the kidney tissues were evaluated, and the level of kidney tissue oxidative stress was detected. The content of inflammatory cytokines was measured by ELISA. The expression of ERK1/2, JNK1/2, p38, NF-κB, caspase-3, and Bax/Bcl-2 proteins were estimated by Western blot analysis. The results showed that taking AO reduced the contents of serum urea and creatinine in AKI rats and improve the pathological changes and oxidative stress of the kidney tissue in rats. At the same time, AO reduced inflammation and apoptosis during AKI by regulating the MAPK pathway. The data show that AO has a protective effect on the kidneys and may be a potential drug for treating kidney injury.

[1]  N. Mahmoud,et al.  Geraniol protects against cyclosporine A-induced renal injury in rats: Role of Wnt/β-catenin and PPARγ signaling pathways. , 2021, Life sciences.

[2]  Yan-Yan Zhang,et al.  1,8-Cineole: a review of source, biological activities, and application , 2020, Journal of Asian natural products research.

[3]  M. Abdel-Bakky,et al.  Resveratrol reduces gentamicin-induced EMT in the kidney via inhibition of reactive oxygen species and involving TGF-β/Smad pathway. , 2020, Life sciences.

[4]  José M. López,et al.  Understanding MAPK Signaling Pathways in Apoptosis , 2020, International journal of molecular sciences.

[5]  Cuiyan Liu,et al.  Protective effect of Polygonatum sibiricum polysaccharides on gentamicin-induced acute kidney injury in rats via inhibiting p38 MAPK/ATF2 pathway. , 2020, International journal of biological macromolecules.

[6]  Hebatalla I. Ahmed,et al.  Candesartan and epigallocatechin‐3‐gallate ameliorate gentamicin‐induced renal damage in rats through p38‐MAPK and NF‐κB pathways , 2018, Journal of biochemical and molecular toxicology.

[7]  S. Shanmugam,et al.  Monoterpenes modulating cytokines - A review. , 2019, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[8]  H. Arab,et al.  Troxerutin down-regulates KIM-1, modulates p38 MAPK signaling, and enhances renal regenerative capacity in a rat model of gentamycin-induced acute kidney injury. , 2018, Food & function.

[9]  Young‐Hee Kang,et al.  Eucalyptol Inhibits Advanced Glycation End Products‐Induced Disruption of Podocyte Slit Junctions by Suppressing Rage‐Erk‐C‐Myc Signaling Pathway , 2018, Molecular nutrition & food research.

[10]  Fares E. M. Ali,et al.  Reno-protective effects of ursodeoxycholic acid against gentamicin-induced nephrotoxicity through modulation of NF-κB, eNOS and caspase-3 expressions , 2018, Cell and Tissue Research.

[11]  A. Tolkovsky,et al.  Neuronal Cell Death. , 2018, Physiological reviews.

[12]  R. S. Abdelrahman.  Protective effect of apocynin against gentamicin-induced nephrotoxicity in rats , 2018, Human & experimental toxicology.

[13]  Yu-jie Fu,et al.  Rapid extraction of Amomum tsao-ko essential oil and determination of its chemical composition, antioxidant and antimicrobial activities. , 2017, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[14]  W. Zin,et al.  Eucalyptol attenuates cigarette smoke-induced acute lung inflammation and oxidative stress in the mouse. , 2016, Pulmonary pharmacology & therapeutics.

[15]  H. Arab,et al.  Gamma-Glutamyl Cysteine Attenuates Tissue Damage and Enhances Tissue Regeneration in a rat Model of Lead-Induced Nephrotoxicity , 2016, Biological Trace Element Research.

[16]  G. Seol,et al.  Eucalyptol and Its Role in Chronic Diseases. , 2016, Advances in experimental medicine and biology.

[17]  G. Seol,et al.  Eucalyptol suppresses matrix metalloproteinase‐9 expression through an extracellular signal‐regulated kinase‐dependent nuclear factor‐kappa B pathway to exert anti‐inflammatory effects in an acute lung inflammation model , 2015, The Journal of pharmacy and pharmacology.

[18]  Zhaojian Liu,et al.  Isoliensinine induces apoptosis in triple-negative human breast cancer cells through ROS generation and p38 MAPK/JNK activation , 2015, Scientific Reports.

[19]  Kewu Zeng,et al.  Induction of hepatoma carcinoma cell apoptosis through activation of the JNK-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-ROS self-driven death signal circuit. , 2014, Cancer letters.

[20]  M. Raish,et al.  Beetroot (Beta vulgaris L.) Extract Ameliorates Gentamicin-Induced Nephrotoxicity Associated Oxidative Stress, Inflammation, and Apoptosis in Rodent Model , 2014, Mediators of inflammation.

[21]  U. Juergens.  Anti-inflammatory Properties of the Monoterpene 1.8-cineole: Current Evidence for Co-medication in Inflammatory Airway Diseases , 2014, Drug Research.

[22]  J. M. Kumar,et al.  Naringin ameliorates gentamicin-induced nephrotoxicity and associated mitochondrial dysfunction, apoptosis and inflammation in rats: possible mechanism of nephroprotection. , 2014, Toxicology and applied pharmacology.

[23]  Z. Deng,et al.  Chemical constituents and insecticidal activities of the essential oil from Amomum tsaoko against two stored-product insects. , 2014, Journal of oleo science.

[24]  F. Islam,et al.  1,8-Cineole (Eucalyptol) Mitigates Inflammation in Amyloid Beta Toxicated PC12 Cells: Relevance to Alzheimer’s Disease , 2014, Neurochemical Research.

[25]  Jianbo Sun,et al.  1,8-Cineol Attenuates LPS-Induced Acute Pulmonary Inflammation in Mice , 2013, Inflammation.

[26]  Rafael Kramann,et al.  Differentiated kidney epithelial cells repair injured proximal tubule , 2013, Proceedings of the National Academy of Sciences.

[27]  K. Hua,et al.  Citral Is Renoprotective for Focal Segmental Glomerulosclerosis by Inhibiting Oxidative Stress and Apoptosis and Activating Nrf2 Pathway in Mice , 2013, PloS one.

[28]  G. A. de Castro Brito,et al.  1,8-cineole (eucalyptol) ameliorates cerulein-induced acute pancreatitis via modulation of cytokines, oxidative stress and NF-κB activity in mice. , 2013, Life sciences.

[29]  T. Ma,et al.  Function of the p38MAPK-HSP27 Pathway in Rat Lung Injury Induced by Acute Ischemic Kidney Injury , 2013, BioMed research international.

[30]  D. P. de Sousa,et al.  A Review on Anti-Inflammatory Activity of Monoterpenes , 2013, Molecules.

[31]  S. Norgren,et al.  Protective Effects of N-Acetylcysteine Amide (NACA) on Gentamicin-Induced Apoptosis in LLC-PK1 Cells , 2012, Renal failure.

[32]  J. Bonventre,et al.  Cellular pathophysiology of ischemic acute kidney injury. , 2011, The Journal of clinical investigation.

[33]  H. Oguzturk,et al.  Antioxidative effects of curcumin, β-myrcene and 1,8-cineole against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rats liver , 2011, Toxicology and industrial health.

[34]  A. Thangathirupathi,et al.  Gentamicin-induced nephrotoxicity: Do we have a promising therapeutic approach to blunt it? , 2010, Pharmacological research.

[35]  E. Farombi,et al.  Phenolic extract of soybean (Glycine max) attenuates cisplatin-induced nephrotoxicity in rats. , 2010, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[36]  N. Kopyt,et al.  Acute Kidney Injury: New Concepts in Definition, Diagnosis, Pathophysiology, and Treatment , 2009, The Journal of the American Osteopathic Association.

[37]  P. Palevsky,et al.  Treatment of acute kidney injury: an update on the management of renal replacement therapy , 2007, Current opinion in nephrology and hypertension.

[38]  J. Frøkiaer,et al.  Dysregulation of renal sodium transporters in gentamicin-treated rats. , 2006, Kidney international.

[39]  Seung-Wook Ryu,et al.  JNK- and p38 Kinase-mediated Phosphorylation of Bax Leads to Its Activation and Mitochondrial Translocation and to Apoptosis of Human Hepatoma HepG2 Cells* , 2006, Journal of Biological Chemistry.

[40]  A. Seguro,et al.  Effect of cyclooxygenase inhibitors on gentamicin-induced nephrotoxicity in rats. , 2004, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[41]  J. Pedraza-Chaverri,et al.  BMC Clinical Pharmacology BioMed Central , 2004 .

[42]  Fuminori Tsuruta,et al.  JNK promotes Bax translocation to mitochondria through phosphorylation of 14‐3‐3 proteins , 2004, The EMBO journal.

[43]  R. C. L. Lima Júnior,et al.  1,8-cineole (eucalyptol), a monoterpene oxide attenuates the colonic damage in rats on acute TNBS-colitis. , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[44]  M. Takano,et al.  Molecular aspects of renal handling of aminoglycosides and strategies for preventing the nephrotoxicity. , 2004, Drug metabolism and pharmacokinetics.

[45]  J. Liao,et al.  Differential regulation of endothelial cell adhesion molecule expression by nitric oxide donors and antioxidants , 1998, Journal of leukocyte biology.

[46]  G. Laurent,et al.  Aminoglycoside-induced renal phospholipidosis and nephrotoxicity. , 1990, Biochemical pharmacology.

[47]  S. Shah,et al.  Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. , 1988, The Journal of clinical investigation.

[48]  N. Fineberg,et al.  Aminoglycoside accumulation kinetics in rat renal parenchyma , 1983, Antimicrobial Agents and Chemotherapy.

[49]  Jerome J. Schentag,et al.  Urinary casts as an indicator of renal tubular damage in patients receiving aminoglycosides , 1979, Antimicrobial Agents and Chemotherapy.