Amperometric biosensor for polyphenol based on horseradish peroxidase immobilized on gold electrodes

Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 µM with a linear relationship up to 25 µM. The reduction current of oxidized polyphenols, formed during the enzymatic oxidation of polyphenolic compounds in the presence of H2O2, is proportional to their concentration. The sensitivity of the detection of various polyphenols by the present method depends on both the electron-donating properties of polyphenols and the electron-accepting properties of oxidized polyphenols. The total amounts of polyphenols in several wine and tea samples detected by the present method are well correlated with those determined by the Folin-Ciocalteu method. In addition, this method has several advantages over the Folin-Ciocalteu method: shorter detection time, smaller sample volume, and more torelant to interference substances.

[1]  H. Abruña,et al.  Dithiobissuccinimidyl propionate as an anchor for assembling peroxidases at electrodes surfaces and its application in a H2O2 biosensor. , 1999, Analytical chemistry.

[2]  S. Cosnier,et al.  Development of a PPO-poly(amphiphilic pyrrole) electrode for on site monitoring of phenol in aqueous effluents , 1999 .

[3]  M. Ding,et al.  Rapid, direct determination of polyphenols in tea by reversed-phase column liquid chromatography. , 1999, Journal of chromatography. A.

[4]  Concepción Sánchez-Moreno,et al.  Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents , 1999 .

[5]  S. Delpal,et al.  Antioxidant activity of resveratrol and alcohol-free wine polyphenols related to LDL oxidation and polyunsaturated fatty acids. , 1999, Life sciences.

[6]  P. Stremple,et al.  Analysis of (+)-catechin, (-)-epicatechin and their 3'- and 4'-O-methylated analogs. A comparison of sensitive methods. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[7]  Hisako Arai,et al.  On-line screening method for antioxidants by liquid chromatography with chemiluminescence detection , 1999 .

[8]  G. Beecher,et al.  Method for determining the content of catechins in tea infusions by high-performance liquid chromatography. , 1998, Journal of chromatography. A.

[9]  P. Teissèdre,et al.  Comparison of (+)-catechin determination in human plasma by high-performance liquid chromatography with two types of detection: fluorescence and ultraviolet. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[10]  K. Kano,et al.  Peroxidase-based amperometric sensor of hydrogen peroxide generated in oxidase reaction: Application to creatinine and creatine assay , 1997 .

[11]  L. Gorton,et al.  Amperometric detection of phenols using peroxidase-modified graphite electrodes , 1997 .

[12]  S. Dong,et al.  The electrochemical study of oxidation-reduction properties of horseradish peroxidase , 1997 .

[13]  Zhen-Yu Chen,et al.  Inhibitory effect of jasmine green tea epicatechin isomers on LDL-oxidation , 1997 .

[14]  D. Hobara,et al.  Reductive Desorption of Carboxylic acid-Terminated Alkanethiol Monolayers from Au(111) Surfaces , 1997 .

[15]  K. Nakagawa,et al.  Chemiluminescence-high-performance liquid chromatographic determination of tea catechin, (-)-epigallocatechin 3-gallate, at picomole levels in rat and human plasma. , 1997, Analytical biochemistry.

[16]  C. Rice-Evans,et al.  Antioxidant properties of phenolic compounds , 1997 .

[17]  Jenny Emnéus,et al.  Peroxidase-modified electrodes: Fundamentals and application , 1996 .

[18]  L. Gorton,et al.  The development of a peroxidase biosensor for monitoring phenol and related aromatic compounds , 1995 .

[19]  K. G. Olsen,et al.  Self-assembled monolayers and enzyme electrodes: Progress, problems and prospects , 1995 .

[20]  K. Hsu,et al.  Determination of (+)-catechin in plasma by high-performance liquid chromatography using fluorescence detection. , 1995, Journal of chromatography. B, Biomedical applications.

[21]  L. Gorton,et al.  Phenol oxidase-based biosensors as selective detection units in column liquid chromatography for the determination of phenolic compounds , 1994 .

[22]  V. Cerdà,et al.  Enhanced automatic flow-injection determination of the total polyphenol index in wines using Folin-Ciocalteu reagent , 1992 .

[23]  L. Foo,et al.  Antioxidant and radical scavenging activities of polyphenols from apple pomace. , 2000 .

[24]  Huafu Wang,et al.  Isocratic elution system for the determination of catechins, caffeine and gallic acid in green tea using HPLC , 2000 .

[25]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .

[26]  D. Goldberg,et al.  Catechin and Epicatechin Concentrations of Red Wines: Regional and Cultivar-Related Differences , 1998, American Journal of Enology and Viticulture.

[27]  F. Nanjo,et al.  Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. , 1996, Free radical biology & medicine.

[28]  E. Nieminen,et al.  Simultaneous determination of phenol, cresols and xylenols in workplace air, using a polystyrene-divinylbenzene column and electrochemical detection. , 1986, Journal of chromatography.