Water holding and release properties of bacterial cellulose obtained by in situ and ex situ modification

Abstract The biomedical applications of bacterial cellulose (BC) as a dressing material are mainly dependent on its water holding capacity (WHC) and water release rate (WRR), which in turn depend on pore size, pore volume and surface area. In the present study, the effects of structural modifications (in situ and ex situ) of BC on the WHC and WRR were investigated. The in situ modified BC was produced by the addition of various concentrations of a single sugar α-linked glucuronic acid-based oligosaccharide (SSGO) to the culture media while the ex situ modifications were carried out by preparing BC composites with chitosan (Ch) and montmorillonite (MMT). The morphological characteristics of the modified BC samples were studied with FE-SEM while their pore size, pore volume and surface area were determined through BET analysis. The results showed modifications in micro-fibril arrangements, pore size, pore volume and surface area in all BC samples compared to the control. The in situ modified BC showed denser fibril arrangement and decreasing pore size and pore volume with increasing SSGO concentration. Pore size and volume were also reduced in BC composites, probably due to the filling of pores by MMT and Ch. The variations in the WHC and WRR of BC samples were correlated to changes in various parameters after structural modifications. The WHC and WRR increased with pore volume and pore size in in situ modified BC samples. In ex situ modified BC, the WHC and WRR were dependent on the nature and arrangement of the composite materials on the surface and in the matrix of the BC sheets.

[1]  W. Wan,et al.  Bacterial cellulose and its nanocomposites for biomedical applications , 2006 .

[2]  B. Theng.  Interactions of Clay Minerals with Organic Polymers. Some Practical Applications , 1970 .

[3]  Kunihiko Watanabe,et al.  Structural Features and Properties of Bacterial Cellulose Produced in Agitated Culture , 1998 .

[4]  Dieter Klemm,et al.  Bacterial synthesized cellulose — artificial blood vessels for microsurgery , 2001 .

[5]  M. Ul-Islam,et al.  Bacterial cellulose production from a single sugar α-linked glucuronic acid-based oligosaccharide , 2011 .

[6]  T. Khan,et al.  Simple fed-batch cultivation strategy for the enhanced production of a single-sugar glucuronic acid-based oligosaccharides by a cellulose-producing Gluconacetobacter hansenii strain , 2008 .

[7]  Seong-Jun Kim,et al.  Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor , 2009 .

[8]  Marek Kawecki,et al.  The future prospects of microbial cellulose in biomedical applications. , 2007, Biomacromolecules.

[9]  Shih-bin Lin,et al.  Adding enzymatically modified gelatin to enhance the rehydration abilities and mechanical properties of bacterial cellulose , 2009 .

[10]  Hiroyuki Yamamoto,et al.  Microstructural analysis of microfibrils of bacterial cellulose , 1997 .

[11]  T. Khan,et al.  Production of bacterial cellulose in static conditions by a simple fed-batch cultivation strategy , 2009 .

[12]  R. Khajavi,et al.  The effects of cotton gauze coating with microbial cellulose , 2010 .

[13]  T. Khan,et al.  Physicochemical and mechanical characterization of bacterial cellulose produced with an excellent productivity in static conditions using a simple fed-batch cultivation strategy , 2010 .

[14]  Haiyang Li,et al.  Determination of the stoichiometry and critical oxygen tension in the production culture of bacterial cellulose using saccharified food wastes , 2011 .

[15]  R. Brown,et al.  Microbial cellulose--the natural power to heal wounds. , 2006, Biomaterials.

[16]  R. Kaomongkolgit,et al.  Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film , 2006, Biotechnology progress.

[17]  S. Kaewnopparat,et al.  Behavior of Freezable Bound Water in the Bacterial Cellulose Produced by Acetobacter xylinum: An Approach Using Thermoporosimetry , 2008, AAPS PharmSciTech.

[18]  T. Khan,et al.  Structural studies of the glucuronic acid oligomers produced by Gluconacetobacter hansenii strain , 2006 .

[19]  Eduardo Ruiz-Hitzky,et al.  Biopolymer−Clay Nanocomposites Based on Chitosan Intercalated in Montmorillonite , 2003 .

[20]  Yuan Zhang,et al.  Potentiality of Bacterial Cellulose as the Scaffold of Tissue Engineering of Cornea , 2009, 2009 2nd International Conference on Biomedical Engineering and Informatics.

[21]  M. Phisalaphong,et al.  Novel nanoporous membranes from regenerated bacterial cellulose , 2008 .

[22]  F. Yoshinaga,et al.  Relationship between Suspension Properties and Fibril Structure of Disintegrated Bacterial Cellulose. , 1998, Bioscience, biotechnology, and biochemistry.

[23]  Weihua Tang,et al.  The influence of fermentation conditions and post-treatment methods on porosity of bacterial cellulose membrane , 2009 .

[24]  Y. Dahman.  Nanostructured biomaterials and biocomposites from bacterial cellulose nanofibers. , 2009, Journal of nanoscience and nanotechnology.

[25]  P. Gostomski,et al.  Determining the Water Holding Capacity of Microbial Cellulose , 2005, Biotechnology Letters.

[26]  J. Catchmark,et al.  Surface area and porosity of acid hydrolyzed cellulose nanowhiskers and cellulose produced by Gluconacetobacter xylinus , 2012 .

[27]  Y. Wan,et al.  Preparation and characterization of bacterial cellulose sponge with hierarchical pore structure as tissue engineering scaffold , 2011 .

[28]  K. Cheng,et al.  Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property , 2009 .

[29]  P. Gatenholm,et al.  Characterization of water in bacterial cellulose using dielectric spectroscopy and electron microscopy , 2007 .

[30]  Jaehwan Kim,et al.  Preparation and characterization of a Bacterial cellulose/Chitosan composite for potential biomedical application , 2011 .

[31]  D. Ciechańska,et al.  Multifunctional Bacterial Cellulose/Chitosan Composite Materials for Medical Applications , 2004 .

[32]  M. Phisalaphong,et al.  Novo aloe vera–bacterial cellulose composite film from biosynthesis , 2010 .

[33]  C. Marone,et al.  Effect of hydration state on the frictional properties of montmorillonite-based fault gouge , 2007 .

[34]  Joong Kon Park,et al.  Effect of reactor surface on production of bacterial cellulose and water soluble oligosaccharides by Gluconacetobacter hansenii PJK , 2010 .

[35]  Mazhar Ul-Islam,et al.  Effect of chitosan penetration on physico-chemical and mechanical properties of bacterial cellulose , 2011 .