Effect of lignin content on enzymatic hydrolysis of furfural residues.

The enzymatic saccharification of pretreated furfural residues with different lignin content was studied to verify the effect of lignin removal in the hydrolysis process. The results showed that the glucose yield was improved by increasing the lignin removal. A maximum glucose yield of 96.8% was obtained when the residue with a lignin removal of 51.4% was hydrolyzed for 108 h at an enzyme loading of 25 FPU/g cellulose. However, further lignin removal did not increase the hydrolysis. The effect of enzyme loading on the enzymatic hydrolysis was also explored in this work. It was concluded that a high glucose yield of 90% was achieved when the enzyme dosage was reduced from 25 to 15 FPU/g cellulose, which was cost-effective for the sugar and ethanol production. The structures of raw material and delignified samples were further characterized by XRD and scanning electron microscopy (SEM).

[1]  A. Converse,et al.  Adsorption of cellulase from Trichoderma reesei on cellulose and lignacious residue in wood pretreated by dilute sulfuric acid with explosive decompression , 1990, Biotechnology and bioengineering.

[2]  E. Cowling.  Physical and chemical constraints in the hydrolysis of cellulose and lignocellulosic materials. , 1975, Biotechnology and bioengineering symposium.

[3]  M. Ballesteros,et al.  Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875 , 2004 .

[4]  V. Puri.  Effect of crystallinity and degree of polymerization of cellulose on enzymatic saccharification , 1984, Biotechnology and bioengineering.

[5]  S J Duff,et al.  Effect of oxygen delignification on the rate and extent of enzymatic hydrolysis of lignocellulosic material. , 2001, Bioresource technology.

[6]  D. Fengel,et al.  Characterization of the Acid Pretreatement for the Enzymatic Hydrolysis of Wood , 1988 .

[7]  John N. Saddler,et al.  The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods , 1998 .

[8]  L Meunier-Goddik,et al.  Enzyme-catalyzed saccharification of model celluloses in the presence of lignacious residues. , 1999, Journal of agricultural and food chemistry.

[9]  M. Holtzapple,et al.  Fundamental factors affecting biomass enzymatic reactivity , 2000, Applied biochemistry and biotechnology.

[10]  Cowling Eb.  Physical and chemical constraints in the hydrolysis of cellulose and lignocellulosic materials. , 1975 .

[11]  P. Weimer,et al.  Effects of chemical treatments and heating on the crystallinity of celluloses and their implications for evaluating the effect of crystallinity on cellulose biodegradation , 1995, Biotechnology and bioengineering.

[12]  Johan Börjesson,et al.  Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose , 2002 .

[13]  J. Saddler,et al.  Effect of enzymatic hydrolysis on the morphology and fine structure of pretreated cellulosic residues , 1993 .

[14]  Mark F. Davis,et al.  Can delignification decrease cellulose digestibility in acid pretreated corn stover? , 2009 .

[15]  L. Fan,et al.  The influence of major structural features of cellulose on rate of enzymatic hydrolysis , 1981 .

[16]  J. Saddler,et al.  Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis , 1999, Biotechnology progress.

[17]  M. M. Gharpuray,et al.  Structural modification of lignocellulosics by pretreatments to enhance enzymatic hydrolysis , 1983, Biotechnology and bioengineering.

[18]  R. Sun,et al.  Influence of steaming pressure on steam explosion pretreatment of Lespedeza stalks (Lespedeza crytobotrya): Part 1. Characteristics of degraded cellulose , 2009 .

[19]  James D. McMillan,et al.  Pretreatment of lignocellulosic biomass , 1994 .