Ribosomal DNA Sequence-Based Taxonomy and Antimicrobial Activity of Prorocentrum spp. (Dinophyceae) from Mauritius Coastal Waters, South-West Indian Ocean

Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains of Prorocentrum species were isolated from macrophytes and cultured under standard laboratory conditions. Morphologies were examined by light, fluorescence, and scanning electron microscopy, and phylogenetic analyses were based on partial large subunit LSU rDNA (D1-D2) and ITS1-5.8S-ITS2 (ITS) regions. Three Prorocentrum species, including the P. fukuyoi complex, P. rhathymum, and P. lima complex, were identified. The antimicrobial activities were assayed against potential human pathogenic bacterial strains. The highest zone of inhibition was recorded for intracellular and extracellular protein extracts of Prorocentrum rhathymum against Vibrio parahaemolyticus. The polysaccharide extracts of the Prorocentrum fukuyoi complex had a higher zone of inhibition (24 ± 0.4 mm) against MRSA at a minimum concentration of 0.625 μg/mL. The extracts from the three Prorocentrum species had different levels of activity against the pathogens used, and this can be of scientific interest in the search for antibiotics from natural marine sources.

[1]  R. Jeewon,et al.  Molecular Characterization and Morpho-Taxonomy of Gambierdiscus caribaeus Vandersea, Litaker, M.A.Faust, Kibler, W.C.Holland & P.A.Tester (Dinophyceae) from Mauritius Island, South-West Indian Ocean , 2023, Cryptogamie, Algologie.

[2]  A. Amato,et al.  Morphology and phylogeny of Prorocentrum porosum sp. nov. (Dinophyceae): A new benthic toxic dinoflagellate from the Atlantic and Pacific Oceans. , 2023, Harmful algae.

[3]  C. Leaw,et al.  Cryptic speciation of benthic Prorocentrum (Dinophyceae) species and their potential as ecological indicators , 2022, Journal of Sea Research.

[4]  Baiju G Nair,et al.  Promising bioactive compounds from the marine environment and their potential effects on various diseases , 2022, Journal of Genetic Engineering and Biotechnology.

[5]  Hui Liu,et al.  Taxonomy and toxin profile of harmful benthic Prorocentrum (Dinophyceae) species from the Xisha Islands, South China Sea , 2021, Journal of Oceanology and Limnology.

[6]  D. Camacho-Muñoz,et al.  High Value Phycotoxins From the Dinoflagellate Prorocentrum , 2021, Frontiers in Marine Science.

[7]  S. Mandal,et al.  Taxonomy and okadaic acid production of a strain of Prorocentrum lima (Dinophyceae) isolated from the Bay of Bengal, North Indian Ocean. , 2021, Toxicon : official journal of the International Society on Toxinology.

[8]  K. Hyde,et al.  Investigating species boundaries in Colletotrichum , 2021, Fungal Diversity.

[9]  G. Hallegraeff,et al.  Global harmful algal bloom status reporting. , 2021, Harmful algae.

[10]  H. Madduppa,et al.  Morphotype diversity of Prorocentrum lima in the western part of Indonesian waters , 2021 .

[11]  H. Uchida,et al.  Abundance of the benthic dinoflagellate Prorocentrum and the diversity, distribution, and diarrhetic shellfish toxin production of Prorocentrum lima complex and P. caipirignum in Japan. , 2020, Harmful algae.

[12]  P. Costa,et al.  Global impact of ciguatoxins and ciguatera fish poisoning on fish, fisheries and consumers. , 2020, Environmental research.

[13]  S. Mudliar,et al.  Recent Advances in Microalgal Bioactives for Food, Feed, and Healthcare Products: Commercial Potential, Market Space, and Sustainability. , 2019, Comprehensive reviews in food science and food safety.

[14]  S. Murray,et al.  Morphology and Phylogenetics of Benthic Prorocentrum Species (Dinophyceae) from Tropical Northwestern Australia , 2019, Toxins.

[15]  L. Chan,et al.  Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae). , 2019, Harmful algae.

[16]  M. Perälä,et al.  Marine Microalgae: Promising Source for New Bioactive Compounds , 2018, Marine drugs.

[17]  H. Uchida,et al.  Variations in morphology, growth, and toxicity among strains of the Prorocentrum lima species complex isolated from Cuba and Brazil , 2018, Journal of Applied Phycology.

[18]  N. Chomérat,et al.  A taxonomical study of benthic Prorocentrum species (Prorocentrales, Dinophyceae) from Anse Dufour (Martinique Island, eastern Caribbean Sea) , 2018, Marine Biodiversity.

[19]  Sudhir Kumar,et al.  MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. , 2018, Molecular biology and evolution.

[20]  S. Fraga,et al.  Morphology and phylogeny of Prorocentrum caipirignum sp. nov. (Dinophyceae), a new tropical toxic benthic dinoflagellate. , 2017, Harmful algae.

[21]  Senjie Lin,et al.  The Synonymy of the Toxic Dinoflagellates Prorocentrum mexicanum and P. rhathymum and the Description of P. steidingerae sp. nov. (Prorocentrales, Dinophyceae) , 2017, The Journal of eukaryotic microbiology.

[22]  K. Hyde,et al.  Molecular Phylogeny and Morphological Characterization of Asexual Fungi (Tubeufiaceae) from Freshwater Habitats in Yunnan, China , 2017, Cryptogamie, Mycologie.

[23]  Haifeng Gu,et al.  Morphology, molecular phylogeny and okadaic acid production of epibenthic Prorocentrum(Dinophyceae) species from the northern South China Sea , 2017 .

[24]  S. Morris,et al.  Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization. , 2016, Harmful algae.

[25]  F. Esposito,et al.  Bioactivity Screening of Microalgae for Antioxidant, Anti-Inflammatory, Anticancer, Anti-Diabetes, and Antibacterial Activities , 2016, Front. Mar. Sci..

[26]  Yang Li,et al.  Morphotypes of Prorocentrum lima (Dinophyceae) from Hainan Island, South China Sea: morphological and molecular characterization , 2015 .

[27]  L. Medlin,et al.  Are Prorocentrum hoffmannianum and Prorocentrum belizeanum (DINOPHYCEAE, PROROCENTRALES), the same species? An integration of morphological and molecular data , 2015, Journal of phycology.

[28]  Y. Jeon,et al.  Potentiality of benthic dinoflagellate cultures and screening of their bioactivities in Jeju Island, Korea , 2014 .

[29]  M. Schweikert,et al.  Taxonomy and phylogeny of the benthic Prorocentrum species (Dinophyceae)—A proposal and review , 2013 .

[30]  A. C. Guedes,et al.  Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae , 2013, Marine drugs.

[31]  Y. Jeon,et al.  In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae , 2013 .

[32]  A. Penna,et al.  Investigation of toxin profile of Mediterranean and Atlantic strains of Ostreopsis cf. siamensis (Dinophyceae) by liquid chromatography–high resolution mass spectrometry , 2013 .

[33]  E. Berdalet,et al.  Global Ecology and Oceanography of Harmful Algal Blooms, GEOHAB Core Research Project: HABs in Benthic Systems , 2012 .

[34]  Shane S. Sturrock,et al.  Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data , 2012, Bioinform..

[35]  S. Murray,et al.  SPECIES BOUNDARIES IN THE TOXIC DINOFLAGELLATE PROROCENTRUM LIMA (DINOPHYCEAE, PROROCENTRALES), BASED ON MORPHOLOGICAL AND PHYLOGENETIC CHARACTERS 1 , 2011, Journal of phycology.

[36]  K. Hyde,et al.  Sequence data reveals phylogenetic affinities of fungal anamorphs Bahusutrabeeja, Diplococcium, Natarajania, Paliphora, Polyschema, Rattania and Spadicoides , 2010, Fungal Diversity.

[37]  Stacey M Etheridge,et al.  Paralytic shellfish poisoning: seafood safety and human health perspectives. , 2010, Toxicon : official journal of the International Society on Toxinology.

[38]  B. Sánchez,et al.  A method for the identification of proteins secreted by lactic acid bacteria grown in complex media. , 2009, FEMS microbiology letters.

[39]  K. Hyde,et al.  Multi-gene phylogeny and morphotaxonomy of Amniculicola lignicola: a novel freshwater fungus from France and its relationships to the Pleosporales. , 2008, Mycological research.

[40]  M. Richlen,et al.  A technique for the rapid extraction of microalgal DNA from single live and preserved cells. , 2005 .

[41]  S. Morris,et al.  Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK. , 2005, Toxicon : official journal of the International Society on Toxinology.

[42]  Ø. Moestrup,et al.  Marine epibenthic dinoflagellates from Malaysia‐a study of live cultures and preserved samples based on light and scanning electron microscopy , 2004 .

[43]  S. Puiseux-Dao,et al.  Morphological and toxicological variability of Prorocentrum lima clones isolated from four locations in the south-west Indian Ocean. , 2001, Toxicon : official journal of the International Society on Toxinology.

[44]  John P. Huelsenbeck,et al.  MRBAYES: Bayesian inference of phylogenetic trees , 2001, Bioinform..

[45]  D. Anderson,et al.  IDENTIFICATION OF GROUP‐ AND STRAIN‐SPECIFIC GENETIC MARKERS FOR GLOBALLY DISTRIBUTED ALEXANDRIUM (DINOPHYCEAE). II. SEQUENCE ANALYSIS OF A FRAGMENT OF THE LSU rRNA GENE 1 , 1994 .

[46]  M. A. Faust MORPHOLOGY OF CIGUATERA‐CAUSING PROROCENTRUM LIMA (PYRROPHYTA) FROM WIDELY DIFFERING SITES 1 , 1991 .

[47]  M. A. Faust MORPHOLOGIC DETAILS OF SIX BENTHIC SPECIES OF PROROCENTRUM (PYRROPHYTA) FROM A MANGROVE ISLAND, TWIN CAYS, BELIZE, INCLUDING TWO NEW SPECIES 1 , 1990 .

[48]  L. Fritz,et al.  A RAPID SIMPLE TECHNIQUE UTILIZING CALCOFLUOR WHITE M2R FOR THE VISUALIZATION OF DINOFLAGELLATE THECAL PLATES 1 , 1985 .

[49]  J. Sherley,et al.  The correct position of flagellar insertion in Prorocentrum and description of Prorocentrum rhathymum sp. nov. (Pyrrhophyta) , 1979 .

[50]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[51]  R. Guillard,et al.  Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. , 1962, Canadian journal of microbiology.

[52]  L. Ferrara Dinoflagellates Important Marine Producers of Natural Bio-Compounds with High Biotechnological and Pharmacological Potential , 2020 .

[53]  G. Dhunnoo,et al.  Spatial and Seasonal Variations of Harmful Benthic Dinoflagellates in the Coastal Waters of Mauritius , 2020 .

[54]  A. Baxevanis,et al.  Potentially toxic epiphytic Prorocentrum (Dinophyceae) species in Greek coastal waters. , 2009 .

[55]  M. Kawachi,et al.  Traditional Microalgae Isolation Techniques , 2005 .

[56]  Jong-Su Yoo Morphological variation of dinoflagellate Prorocentrum lima (Ehrenberg) Dodge with geographical difference. , 2004, Journal of environmental biology.

[57]  Mary Jane Ferraro,et al.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard , 2000 .

[58]  Y. Fukuyo Taxonomical study on benthic dinoflagellates collected in coral reefs. , 1981 .

[59]  International Council for the Exploration of the Sea , 1949, Nature.