Ultra-slim plastic endomicroscope objective for non-linear microscopy

Non-linear microscopy has the potential to provide clinically useful information on the structure of biological tissue in vivo via an endomicroscope. The ability to use plastic as the optical material in a multiphoton objective was evaluated based on several criteria including autofluorescence, injection molding induced birefringence, and pulse broadening due to group velocity dispersion. An all-plastic, refractive ultra-slim endoscope objective was built with design specifications of NA = 0.4, FOV = 250 μm, 1.27 mm outer diameter, and 0.8 mm clear aperture. Initial images of second-harmonic generation signal (illumination at 780 nm) in collagen fibers and two-photon excited fluorescence (illumination at 920 nm) of Convallaria rhizome are reported.

[1]  Technical Note: MRI-guided breast biopsy - our preliminary experience , 2010, The Indian journal of radiology & imaging.

[2]  Robert T Kester,et al.  Low cost, high performance, self-aligning miniature optical systems. , 2009, Applied optics.

[3]  W. Webb,et al.  Multiphoton microscopy in biological research. , 2001, Current opinion in chemical biology.

[4]  K König,et al.  Clinical two‐photon microendoscopy , 2007, Microscopy research and technique.

[5]  W. Denk,et al.  Two-photon laser scanning fluorescence microscopy. , 1990, Science.

[6]  Deepa Narayanan,et al.  PET‐Guided Breast Biopsy , 2011, The breast journal.

[7]  I. Nikolov,et al.  Analysis of the dispersion of optical plastic materials , 2007 .

[8]  Xingde Li,et al.  Combined influences of chromatic aberration and scattering in depth-resolved two-photon fluorescence endospectroscopy , 2010, Biomedical optics express.

[9]  Maria Goeppert-Mayer.  Über Elementarakte mit zwei Quantensprüngen , 1931 .

[10]  L. Pruitt,et al.  In vivo behavior of acrylic bone cement in total hip arthroplasty. , 2006, Biomaterials.

[11]  J. Mooney,et al.  Measurement of the modulation transfer function of infrared cameras , 1995 .

[12]  W. Webb,et al.  Nonlinear magic: multiphoton microscopy in the biosciences , 2003, Nature Biotechnology.

[13]  R Richards-Kortum,et al.  Optical Systems for in Vivo Molecular Imaging of Cancer , 2003, Technology in cancer research & treatment.

[14]  R. Gordon,et al.  Development of a versatile two-photon endoscope for biological imaging , 2010, Biomedical optics express.

[15]  B Messerschmidt,et al.  Nonlinear optical endoscope based on a compact two axes piezo scanner and a miniature objective lens. , 2008, Optics express.

[16]  Sophie Lanone,et al.  Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity , 2011, Particle and Fibre Toxicology.

[17]  Watt W Webb,et al.  Human bladder cancer diagnosis using multiphoton microscopy , 2009, BiOS.

[18]  D. Tank,et al.  A Miniature Head-Mounted Two-Photon Microscope High-Resolution Brain Imaging in Freely Moving Animals , 2001, Neuron.

[19]  F. Helmchen,et al.  Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo. , 2008, Optics express.

[20]  Stefan Bäumer.  Handbook of plastic optics , 2005 .

[21]  A. Mehta,et al.  Multiphoton endoscopy: optical design and application to in vivo imaging of mammalian hippocampal neurons , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..

[22]  M. Sivak,et al.  In vivo optical coherence tomography imaging of the pancreatic and biliary ductal system. , 2005, Gastrointestinal endoscopy.

[23]  Jun Nagata,et al.  Multiphoton imaging can be used for microscopic examination of intact human gastrointestinal mucosa ex vivo. , 2008, Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association.

[24]  Xingde Li,et al.  Fiber-optic scanning two-photon fluorescence endoscope. , 2006, Optics letters.

[25]  L. Fu,et al.  Nonlinear optical endoscopy based on a double-clad photonic crystal fiber and a MEMS mirror. , 2006, Optics express.

[26]  Matthew D. Chidley,et al.  Design, assembly, and optical bench testing of a high-numerical-aperture miniature injection-molded objective for fiber-optic confocal reflectance microscopy. , 2006, Applied optics.

[27]  Chi-Kuang Sun,et al.  Nonlinear (Harmonic Generation) Optical Microscopy , 2006 .

[28]  Ho-Young Park,et al.  Accuracy of preoperative ultrasound and ultrasound‐guided fine needle aspiration cytology for axillary staging in breast cancer , 2010, ANZ journal of surgery.

[29]  Karsten König,et al.  Rigid and high NA multiphoton fluorescence GRIN-endoscopes , 2007, European Conference on Biomedical Optics.

[30]  Koji Minami,et al.  High performance cyclo olefin polymer ZEONEX , 2005, SPIE Optics + Photonics.

[31]  M. Petrtýl,et al.  Cycloolefin-Copolymer/Polyethylene (COC/PE) Blend Assists with the Creation of New Articular Cartilage , 2010 .

[32]  Robert T Kester,et al.  High numerical aperture microendoscope objective for a fiber confocal reflectance microscope. , 2007, Optics express.

[33]  R. Peterson,et al.  Hydrolytic degradation of tyrosine-derived polycarbonates, a class of new biomaterials. Part II: 3-yr study of polymeric devices. , 2000, Biomaterials.

[34]  Tomasz S. Tkaczyk,et al.  Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy , 2010, Optics express.

[35]  Hui Ma,et al.  Reconstruction of complementary images in second harmonic generation microscopy. , 2006, Optics express.

[36]  Masaaki Komatsu,et al.  High performance optical materials cyclo olefin polymer ZEONEX , 2007, SPIE Optical Engineering + Applications.

[37]  W. Stryjewski,et al.  Single molecule detection of double‐stranded DNA in poly(methylmethacrylate) and polycarbonate microfluidic devices , 2001, Electrophoresis.

[38]  Xin Li,et al.  Deep Tissue Microscopic Imaging of the Kidney with a Gradient-Index Lens System. , 2008, Optics communications.

[39]  Anthony F. Garito,et al.  Origin of high bandwidth performance of graded-index plastic optical fibers , 1997 .

[40]  F. Helmchen,et al.  Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective. , 2004, Optics letters.

[41]  M. Mansuripur.  Classical Optics and Its Applications: Effect of polarization on diffraction in systems of high numerical aperture , 2002 .

[42]  Min Gu,et al.  Two-photon fluorescence endoscopy with a micro-optic scanning head. , 2003, Optics letters.

[43]  A. Zvyagin.  Multiphoton endoscopy , 2007 .