Self-expansion behavior of silk fibroin film

Silk fibroin cast film was prepared using a ternary solvent system of CaCl 2 /CH 3 CH 2 OH/H 2 O (1/2/8 in mole ratio). A drying temperature at casting influenced crystal structure of fibroin. When a drying temperature was set lower than 9 °C, the cast film became amorphous. When a drying temperature was set higher than 40 °C, a fibroin film of silk-II structure was obtained. In order to produce a fibroin film of silk-I structure, a preferable temperature range was from 20 to 26 °C. The crystal transformation from random coil structure into silk-I could be made through exposure of an amorphous film to water vapor. As for the crystal transformation from silk-I into silk-II, the treatment with a glycerin solution was effective. In the course of the treatment a film showed self-thinning and self-expanding. The expansion ratio exceeded 40% at maximum. The film produced accompanying self-expansion was ductile in nature.

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