Dinitrophenol-Stimulated Adenosine Triphosphatase Activity in Extracts of Desulfovibrio gigas

A dinitrophenol (DNP)-stimulated adenosine triphosphatase (ATPase) has been found in both the soluble and particulate fractions of the anaerobic sulfate-reducing bacterium, Desulfovibrio gigas. As the soluble ATPase was labile to storage, only the particulate enzyme was studied in detail. It was optimally stimulated by DNP at 4 mm, and activity was insensitive to inhibition by ouabain. The ATPase was stimulated by both Ca2+ and Mg2+, but the magnitude of the stimulation was dependent upon pH. In the presence of Ca2+ the optimum pH was 6.5, whereas, in the presence of Mg2+ the pH optimum was 8.0. However, under optimal conditions the activity was the same with either Mg2+ or Ca2+. Both adenosine triphosphate and guanosine triphosphate were hydrolyzed, but activity toward guanosine triphosphate was only one-tenth that observed with adenosine triphosphate.

[1]  M. Aleem,et al.  PHOSPHORYLATION COUPLED TO NITRITE OXIDATION BY PARTICLES FROM THE CHEMOAUTOTROPH, NITROBACTER AGILIS. , 1960, Proceedings of the National Academy of Sciences of the United States of America.

[2]  E. C. Slater,et al.  The enzymic hydrolysis of adenosine triphosphate by liver mitochondria. 2. Effect of inhibitors and added cofactors. , 1957, The Biochemical journal.

[3]  E. Racker,et al.  Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation XIV. INTERACTION OF PURIFIED MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE FROM BAKERS' YEAST WITH SUBMITOCHONDRIAL PARTICLES FROM BEEF HEART , 1967 .

[4]  H. D. Peck,et al.  Phosphorylation coupled with electron transfer in extracts of the sulfate reducing bacterium, Desulfovibrio gigas. , 1966, Biochemical and biophysical research communications.

[5]  E. C. Slater,et al.  The enzymic hydrolysis of adenosine triphosphate by liver mitochondria. I. Activities at different pH values. , 1957, The Biochemical journal.

[6]  P. Robbins,et al.  Enzymatic synthesis of adenosine-5'-phosphosulfate. , 1958, The Journal of biological chemistry.

[7]  L. Packer.  Regulation of respiration in heart sarcosomes. , 1958, Experimental cell research.

[8]  E. Racker,et al.  Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation XXVIII. THE RECONSTITUTION OF THE FIRST SITE OF ENERGY CONSERVATION , 1973 .

[9]  M. Katsumata,et al.  POSSIBLE PARTIAL REACTIONS OF THE PHOTOPHOSPHORYLATION PROCESS IN CHROMATOPHORES FROM RHODOSPIRILLUM RUBRUM. , 1965, Biochimica et biophysica acta.

[10]  E. Racker,et al.  Studies on the mechanism of the conversion of coupling factor 1 from chloroplasts to an active adenosine triphosphatase. , 1970, Biochemistry.

[11]  E. Racker,et al.  PARTIAL RESOLUTION OF THE ENZYMES CATALYZINE PHOTOPHOSPHORYLATION. I. STIMULATION OF PHOTOPHOSPHORYLATION BY A PREPARATION OF A LATENT, CA++- DEPENDENT ADENOSINE TRIPHOSPHATASE FROM CHLOROPLASTS. , 1965, The Journal of biological chemistry.

[12]  M. Yates.  Stimulation of the phosphoroclastic system of Desulfovibrio by nucleotide triphosphates. , 1967, The Biochemical journal.

[13]  M. E. Pullman,et al.  Mitochondrial oxidations and energy coupling. , 1967, Annual review of biochemistry.

[14]  E. Racker,et al.  Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase. , 1960, The Journal of biological chemistry.

[15]  N. Coles,et al.  Adenosine Triphosphatase in Isolated Membranes of Staphylococcus aureus , 1968, Journal of bacteriology.

[16]  A. F. Brodie,et al.  Oxidative phosphorylation in fractionated bacterial systems XI. Separation of soluble factors necessary for oxidative phosphorylation , 1963 .

[17]  E. King,et al.  The colorimetric determination of phosphorus. , 1932, The Biochemical journal.

[18]  A. Lehninger,et al.  Reconstitution of Oxidative Phosphorylation in Preparations from Micrococcus lysodeikticus , 1962 .

[19]  H. Gest,et al.  A coupling factor in bacterial photophosphorylation. , 1970, The Journal of biological chemistry.

[20]  A. F. Brodie,et al.  Oxidative phosphorylation in fractionated bacterial systems. XLII. The effect of coupling factors on urea-treated particles from M. phlei. , 1970, Archives of biochemistry and biophysics.

[21]  H. Zalkin,et al.  Partial resolution of the enzymes catalyzing oxidative phosphorylation. V. Properties of coupling factor 4. , 1965, The Journal of biological chemistry.

[22]  L. Barton,et al.  Phosphorylation coupled to oxidation of hydrogen with fumarate in extracts of the sulfate reducing bacterium, Desulfovibrio gigas. , 1970, Biochemical and biophysical research communications.

[23]  A. F. Brodie,et al.  Interchangeability of coupling factors from bacterial and mammalian origin. , 1970, Biochemical and biophysical research communications.

[24]  N. M. Hawkins,et al.  Studies on sodium-potassium-activated adenosinetriphosphatase. IV. Correlation with cation transport sensitive to cardiac glycosides. , 1962, Archives of biochemistry and biophysics.

[25]  C. Hou,et al.  Oxidative phosphorylation in Escherichia coli. , 1968, Canadian journal of biochemistry.

[26]  W. Loomis,et al.  Reversible inhibition of the coupling between phosphorylation and oxidation. , 1948, The Journal of biological chemistry.

[27]  J. L. Gall,et al.  Le cytochrome c3 de Desulfovibrio gigas , 1965 .

[28]  J. Akagi,et al.  STUDIES ON THERMOPHILIC SULFATE-REDUCING BACTERIA III , 1962, Journal of bacteriology.

[29]  R. Levin,et al.  The biuret reaction for the determination of proteins; an improved reagent and its application. , 1951, The Journal of laboratory and clinical medicine.

[30]  H. Gest,et al.  PROPERTIES OF ADENOSINE TRIPHOSPHATASE IN A PHOTOSYNTHETIC BACTERIUM. , 1965, Biochimica et biophysica acta.

[31]  E. C. Slater,et al.  Respiratory Chain Phosphorylation in Extracts of Azotobacter vinelandii , 1955, Nature.

[32]  G. Pinchot,et al.  Phosphorylation coupled to electron transport in cell-free extracts of Alcaligenes faecalis. , 1953, The Journal of biological chemistry.

[33]  A. F. Brodie.  Oxidative phosphorylation in fractionated bacterial systems. I. Role of soluble factors. , 1959, The Journal of biological chemistry.

[34]  E. C. Slater,et al.  The effect of monovalent cations on the dinitrophenol-induced ATPase of rat-liver mitochondria. , 1968, Biochimica et biophysica acta.