Micron Surface-Enhanced Raman Spectroscopy of Intact Biological Organisms and Model Systems

Surface-enhanced Raman spectra have been obtained within intact zebrafish embryos and inside the 500-fL pores of a Nucleopore filter membrane with the use of coated microelectrodes with 1–3 μm active silver tip diameters. The spectra obtained demonstrate the microelectrode's ability to penetrate biological membranes as well as restricted volumes.