Effects of Camellia sinensis Extracts on the Antioxidant System and Alcohol Down-Regulation Enzymes in Sub-Acute Ethanol Treated ICR Mice

This study was conducted to investigate the effects of four kinds of tea (Camellia sinensis) extracts on the antioxidant defense systems as well as the activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in ethanol administered ICR mice. According to the results, treatment with puerh tea significantly increased the superoxide dismutase activity and glutathion reductase activity in liver. In addition, the group treated with oolong tea exhibited higher superoxide dismutase activity and glutathion reductase activity in serum than those of puerh tea, green tea and black tea treated groups. The oolong tea and puerh tea also reduced malondealdehyde contents in both liver and serum. These results suggested that puerh tea and oolong tea were the most effective against alcohol ⁃ induced oxidative damage among the Camellia sinensis teas. On the other hand, in the measurement of alcohol break ⁃ down enzyme activities, the group treated with green tea exhibited the highest hepatic ADH and ALDH activities, suggesting that the group treated with green tea might be useful for alcohol down ⁃ regulation.

[1]  M. Friedman.  Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas , 2006, Molecular nutrition & food research.

[2]  Jen-kun Lin,et al.  Mechanisms of hypolipidemic and anti-obesity effects of tea and tea polyphenols. , 2006, Molecular nutrition & food research.

[3]  E. Skrzydlewska,et al.  Antioxidant properties of black tea in alcohol intoxication. , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[4]  K. Chopra,et al.  Delineation of antimutagenic activity of catechin, epicatechin and green tea extract. , 2004, Mutation research.

[5]  K. Schrör,et al.  Platelet aggregation induced by the C-terminal peptide of thrombospondin-1 (4N1-1) is inhibited by epigallocatechin gallate but not by prostaglandin E1 , 2004, Platelets.

[6]  D. Fung,et al.  Antibacterial effect of water-soluble tea extracts on foodborne pathogens in laboratory medium and in a food model. , 2004, Journal of food protection.

[7]  Insu P. Lee,et al.  Anticancer effects of (-)-epigallocatechin-3-gallate on ovarian carcinoma cell lines. , 2004, Gynecologic oncology.

[8]  Chih-Hsing Wu,et al.  The protective effect of habitual tea consumption on hypertension. , 2004, Archives of internal medicine.

[9]  G. Catignani,et al.  Antioxidants and Prevention of Chronic Disease , 2004, Critical reviews in food science and nutrition.

[10]  J. Vinson,et al.  Green and black teas inhibit atherosclerosis by lipid, antioxidant, and fibrinolytic mechanisms. , 2004, Journal of agricultural and food chemistry.

[11]  H. Noguchi,et al.  Tea Polyphenols Inhibit Acetyl-CoA:1-Alkyl-sn-Glycero-3-Phosphocholine Acetyltransferase (a Key Enzyme in Platelet-Activating Factor Biosynthesis) and Platelet-Activating Factor-Induced Platelet Aggregation , 2004, International Archives of Allergy and Immunology.

[12]  C. Lieber.  The unexpected outcomes of medical research: serendipity and the microsomal ethanol oxidizing system. , 2004, Journal of hepatology.

[13]  M. Indira,et al.  The impact of cyanoglycoside rich fraction isolated from Cassava (Manihot esculenta) on alcohol induced oxidative stress. , 2003, Toxicon : official journal of the International Society on Toxinology.

[14]  K. Fung,et al.  Anti-oxidative, anti-inflammatory and hepato-protective effects of Ligustrum robustum. , 2002, Journal of ethnopharmacology.

[15]  H. Mukhtar,et al.  Photochemoprevention by Botanical Antioxidants , 2002, Skin Pharmacology and Physiology.

[16]  J. Witztum,et al.  Immune responses to oxidative neoepitopes on LDL and phospholipids modulate the development of atherosclerosis , 2000, Journal of internal medicine.

[17]  J. Lin,et al.  Cancer chemoprevention by tea polyphenols through mitotic signal transduction blockade. , 1999, Biochemical pharmacology.

[18]  C. Epstein,et al.  Superoxide-mediated cytotoxicity in superoxide dismutase-deficient fetal fibroblasts. , 1997, Archives of biochemistry and biophysics.

[19]  K. Husain,et al.  Response of cardiac antioxidant system to alcohol and exercise training in the rat. , 1997, Alcohol.

[20]  P. Verger,et al.  Modifications in dietary self-selection specifically attributable to voluntary wheel running and exercise training in the rat , 1996, Physiology & Behavior.

[21]  D. Bredesen,et al.  Altered Reactivity of Superoxide Dismutase in Familial Amyotrophic Lateral Sclerosis , 1996, Science.

[22]  Jen-kun Lin,et al.  Biochemical Studies on the Anti-Carcinogenesis of Tea Polyphenols , 1995 .

[23]  C. Epstein,et al.  Attenuation of focal cerebral ischemic injury in transgenic mice overexpressing CuZn superoxide dismutase. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[24]  K. Yagi,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. , 1979, Analytical biochemistry.

[25]  A. C. Collins,et al.  Preferential inhibition of the low Km aldehyde dehydrogenase activity by pargyline. , 1977, Biochemical pharmacology.

[26]  I. Fridovich,et al.  The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. , 1972, The Journal of biological chemistry.

[27]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[28]  L. Pagliaro.  Milestones in liver disease , 2002 .

[29]  C. Gutiérrez,et al.  Influence of chronic alcohol abuse and liver disease on hepatic aldehyde dehydrogenase activity. , 1998, Alcohol.

[30]  Seung Ho Lee,et al.  Effects of high molecular weight fractions of Aloe spp. on alcohol metabolism , 1998 .

[31]  B. An,et al.  Isolation of a Novel Polyphenol from Oolong Tea and Its Effective Prevention of the Gout , 1998 .

[32]  Su-Rae Lee,et al.  Effect of green tea beverage for the removal of cadmium and lead by animal experiments , 1994 .

[33]  D. J. Reed,et al.  High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives. , 1987, Methods in enzymology.

[34]  M. Dianzani,et al.  Lipid peroxidation in ethanol poisoning: a critical reconsideration. , 1985, Alcohol and alcoholism.

[35]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.

[36]  L. Flohé,et al.  Assays of glutathione peroxidase. , 1984, Methods in enzymology.