Analysis and Purification of Synthetic Nucleic Acids Using HPLC

HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed‐phase HPLC allows a high‐capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion‐exchange HPLC provides better resolution and a more predictable elution pattern. This unit presents protocols that are optimized for HPLC of oligonucleotides. Because of the resolution limits of both reversed‐phase and anion‐exchange HPLC, it can be used for oligonucleotides of up to ˜50 nt in length.

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