Ultrastructural localization of hyaluronic acid in the synovium of the goat knee joint.

In the Japanese miniature (Shiba) goat, the synovial membrane contains synoviocytes referred to as type A (macrophage-like cells) and type B cells (fibroblast-like cells) in the intimal layer. Small capillaries and blood vessels of varying sizes were located in the extracellular matrix in the synovial subintima. The type A cells in the synovium possessed numerous vesicles, vacuoles and lysosomes as well as pinocytotic vesicles. These ultrastructural features indicating phagocytosis showed distinct positive reactions following hyaluronan staining. On the other hand, in the type B cells, hyaluronic acids were present in the surface coat of the plasma membrane and its periphery. Additionally, perivascular connective tissue of the small capillaries and blood vessels and interfibrous matrix contained hyaluronan. The results suggest that hyaluronic acid, in the synovial tissue, is synthesized on the plasma membrane of type B cells, and taken up by type A cells. Moreover, hyaluronan is involved in cellular functions in the synovial connective tissue.

[1]  R. Mason,et al.  Effect of depletion of interstitial hyaluronan on hydraulic conductance in rabbit knee synovium , 1998, The Journal of physiology.

[2]  Graabaek Pm.  Characteristics of the two types of synoviocytes in rat synovial membrane. An ultrastructural study. , 1984 .

[3]  Y. Uchiyama,et al.  Intra- and extracellular localization of hyaluronic acid and proteoglycan constituents (chondroitin sulfate, keratan sulfate, and protein core) in articular cartilage of rabbit tibia. , 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[4]  W. Southwick,et al.  Phagocytosis of colloidal gold by cells of synovial membrane. , 1971, The Journal of bone and joint surgery. American volume.

[5]  A. Tsukise,et al.  Ultracytochemical demonstration of glycoproteins in the canine knee synovium. , 2003, Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft.

[6]  I. Wada,et al.  Ultrastructural study of cells at the synovium-cartilage junction: response of synovial cells of the rat knee joint to intra-articularly injected latex particles. , 1999, Kaibogaku zasshi. Journal of anatomy.

[7]  P. Prehm,et al.  Increased hyaluronate synthesis is required for fibroblast detachment and mitosis. , 1986, The Biochemical journal.

[8]  I. Nakanishi,et al.  Ultrastructure of the mouse synovial membrane. Development and organization of the extracellular matrix. , 1981, Arthritis and rheumatism.

[9]  M. Bayliss,et al.  Zonal distribution of chondroitin-4-sulphate/dermatan sulphate and chondroitin-6-sulphate in normal and diseased human synovium. , 1994, Annals of the rheumatic diseases.

[10]  R. L. O'Connor,et al.  O'Connor's Textbook of Arthroscopic Surgery , 1992 .

[11]  R. Graham,et al.  PROTEIN UPTAKE BY SYNOVIAL CELLS I. ULTRASTRUCTURAL STUDY OF THE FATE OF INTRAARTICULARLY INJECTED PEROXIDASES , 1971, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[12]  M. Bayliss,et al.  Morphological localization of hyaluronan in normal and diseased synovium. , 1991, The Journal of rheumatology.

[13]  J. A. Chapman,et al.  THE UPTAKE OF IRON IN RABBIT SYNOVIAL TISSUE FOLLOWING INTRA-ARTICULAR INJECTION OF IRON DEXTRAN , 1964, The Journal of cell biology.

[14]  P. Mapp,et al.  Fibronectin production by synovial intimal cells , 2004, Rheumatology International.

[15]  Pernille Møller Graab˦k.  Ultrastructural evidence for two distinct types of synoviocytes in rat synovial membrane , 1982 .

[16]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[17]  B. Jasani,et al.  A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope , 1983, The Histochemical Journal.

[18]  P. Prehm.  Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth. , 1983, The Biochemical journal.

[19]  A. Tsukise,et al.  Ultracytochemistry of glycosaminoglycans in the canine knee synovium. , 2001, Annals of Anatomy.

[20]  Y. Uchiyama,et al.  Localization of hyaluronic acid, chondroitin sulfate, and CD44 in rabbit cornea. , 1992, Archives of histology and cytology.

[21]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[22]  P. Prehm.  Hyaluronate is synthesized at plasma membranes. , 1984, The Biochemical journal.

[23]  I. Nakanishi,et al.  Secretory granules of B-cells in the synovial membrane , 1981, Cell and Tissue Research.

[24]  F. N. Ghadially,et al.  A mechanism of formation of desmosome-like structures between synovial intimal cells , 1978, Experientia.

[25]  S. Kumar,et al.  The effect of hyaluronate and its oligosaccharides on endothelial cell proliferation and monolayer integrity. , 1989, Experimental cell research.

[26]  T. Iwanaga,et al.  Morphology and functional roles of synoviocytes in the joint. , 2000, Archives of histology and cytology.

[27]  Hodde Kc,et al.  The morphology of synovial lining of various structures in several species as observed with scanning electron microscopy. , 1990 .

[28]  M. Yaron,et al.  Interrelationship between stimulation of prostaglandin E and hyaluronate production by poly (I) . poly (C) and interferon in synovial fibroblast culture. , 1978, Arthritis and rheumatism.

[29]  M. L. Watson.  Staining of Tissue Sections for Electron Microscopy with Heavy Metals , 1958, The Journal of biophysical and biochemical cytology.

[30]  M. Spycher,et al.  The ultrastructural localization of fibronectin in the lining layer of rheumatoid arthritis synovium: The synthesis of fibronectin by type B lining cells , 2004, Rheumatology International.

[31]  J. Key.  THE MECHANISMS INVOLVED IN THE REMOVAL OF COLLOIDAL AND PARTICULATE CARBON FROM JOINT CAVITIES , 1926 .

[32]  R. Mason,et al.  The Proteoglycans and Glycosaminoglycan Chains of Rabbit Synovium , 1998, The Histochemical Journal.

[33]  A. Tsukise,et al.  Histochemical analyses of glycosaminoglycans in the synovial membrane of the canine knee joint. , 2001, Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft.

[34]  Pernille Møller Graab˦k.  Absorption of intraarticularly injected horseradish peroxidase in synoviocytes of rat synovial membrane: an ultrastructural-cytochemical study. , 1985 .

[35]  F. N. Ghadially,et al.  Ultrastructure of Rabbit Synovial Membrane , 1966, Annals of the rheumatic diseases.

[36]  Y. Uchiyama,et al.  Cytochemical localization of hyaluronic acid in human synovium with special reference to its possible process of degradation. , 1995, Archives of histology and cytology.