Detection of plasmapheresis‐induced platelet activation using monoclonal antibodies

Twelve volunteer plasma donors were studied so as to determine the extent and duration of in vivo platelet activation caused by automated plasmapheresis. Samples obtained immediately before and after donation were mixed with murine monoclonal antibodies PAC-1 and S12, which bind specifically to activated platelets. Antibody binding on platelets was quantitated by flow cytometry. The change in the mean fluorescence intensity (MFI) (MFI after donation minus MFI before donation) was 61 +/- 23 (confidence interval [CI], 48-75) for PAC-1 and 56 +/- 64 (CI, 19-92) for S12 in plasmapheresis donors, as compared to 0.3 +/- 0.8 (PAC-1; CI, -0.2-0.8) and 0.3 +/- 0.9 (S12: CI, -0.3-0.9) in whole blood donors (p less than 0.05). Additional studies showed circulating activated platelets up to 48 hours after plasmapheresis. In contrast to other data, significant platelet activation was demonstrated following plasmapheresis on an automated machine. None of the donors had clinical complications. Nevertheless, it may be appropriate to delay subsequent plasmapheresis and platelet procurement from such donors until evidence of platelet activation has disappeared.

[1]  R. Hillman,et al.  Progressive platelet activation with storage: evidence for shortened survival of activated platelets after transfusion , 1991, Transfusion.

[2]  B. Catimel,et al.  Inhibition of platelet functions by a monoclonal antibody (LYP20) directed against a granule membrane glycoprotein (GMP-140/PADGEM). , 1991, Blood.

[3]  S. Murray,et al.  The Canadian Red Cross Plasmapheresis Donor Safety Program: Changes in Plasma Proteins after Long‐Term Plasmapheresis , 1991, Vox sanguinis.

[4]  J. George,et al.  The clinical importance of acquired abnormalities of platelet function. , 1991, The New England journal of medicine.

[5]  C. Knupp,et al.  Effect of platelet activation inhibitors on the loss of glycoprotein Ib during storage of platelet concentrates. , 1990, The Journal of laboratory and clinical medicine.

[6]  D. Devine.  Novel markers for the detection of platelet activation. , 1990, Transfusion medicine reviews.

[7]  J. O'brien,et al.  Shear-induced platelet aggregation , 1990, The Lancet.

[8]  V. Addonizio.  Platelet function in cardiopulmonary bypass and artificial organs. , 1990, Hematology/oncology clinics of North America.

[9]  J. Kelton,et al.  Adverse reactions in healthy blood donors undergoing plasmapheresis. , 1990, Progress in clinical and biological research.

[10]  L. Corash,et al.  Measurement of platelet activation by fluorescence-activated flow cytometry. , 1990, Blood cells.

[11]  A. P. Bode,et al.  Platelet activation may explain the storage lesion in platelet concentrates. , 1990, Blood cells.

[12]  R. McEver.  Properties of GMP-140, an inducible granule membrane protein of platelets and endothelium. , 1990, Blood cells.

[13]  S. Sekiguchi,et al.  An extensive study of donor plasmapheresis using a membrane method by the special study group of the Japanese Red Cross Society. , 1990, Progress in clinical and biological research.

[14]  C. Abrams,et al.  Direct detection of activated platelets and platelet-derived microparticles in humans. , 1990, Blood.

[15]  C. Lambrew,et al.  Correlated measurement of platelet release and aggregation in whole blood. , 1989, Cytometry.

[16]  L. Parise,et al.  LA Fitzgerald The platelet membrane glycoprotein IIb-IIIa complex , 1988 .

[17]  F. Velasco,et al.  Studies on contact phase and platelet activation during thrombopheresis in donors. Preliminary results of a comparison between continuous and intermittent flow systems , 1988 .

[18]  V. Sinitsyn,et al.  Transitory decrease of platelet count, aggregation and adhesion after plasmapheresis and immunosorption procedures. , 1987, Thrombosis research.

[19]  J. George,et al.  Heterogeneity of platelet secretion in response to thrombin demonstrated by fluorescence flow cytometry. , 1987, Blood.

[20]  J. Hoxie,et al.  DETECTION OF ACTIVATED PLATELETS IN WHOLE BLOOD BY FLOW CYTOMETRY , 1987, Thrombosis and Haemostasis.

[21]  J. Hoxie,et al.  Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. , 1987, Blood.

[22]  J. George,et al.  Platelet surface glycoproteins. Studies on resting and activated platelets and platelet membrane microparticles in normal subjects, and observations in patients during adult respiratory distress syndrome and cardiac surgery. , 1986, The Journal of clinical investigation.

[23]  G. Rock,et al.  Plasma collection using an automated membrane device , 1986, Transfusion.

[24]  J. Ritchie,et al.  Studies of human plate alpha-granule release in vivo. , 1981, Blood.

[25]  E. Hessel,et al.  Mechanism of abnormal bleeding in patients undergoing cardiopulmonary bypass: acquired transient platelet dysfunction associated with selective alpha-granule release. , 1980, Blood.

[26]  P. Mannucci,et al.  Acquired dysfunction due to the circulation of "exhausted" platelets. , 1980, The American journal of medicine.

[27]  F. del Greco,et al.  β-Thromboglobulin in Patients with Chronic Renal Failure: Effect of Hemodialysis , 1980, Thrombosis and Haemostasis.