Molecular Mechanism of Cytokinesis.

We use three complementary approaches to study the molecular basis of cytokinesis. We characterize the structures and biophysical properties the participating proteins to generate hypotheses about the biochemical reactions. Quantitative fluorescence microscopy of live cells expressing fluorescent fusion proteins gives the numbers of each type of protein in whole yeast cells with one second temporal resolution. High‐speed super resolution localization microscopy (FPALM) allows us to image structures at 35 nm resolution in live cells on a time scale of a few seconds. Comparisons of these quantitative measurements in cells with simulations of molecularly explicit mathematical models of the reactions in our biochemical hypothesis usually suggest ways to improve our hypotheses. Our simulations now reproduce the time course of events during the assembly and constriction of the cytokinetic contractile ring as observed in live cells and predict the outcomes of experimental manipulations.

[1]  Daesik Kim,et al.  Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases. , 2019, Annual review of biochemistry.

[2]  Nir Kalisman,et al.  Integrative Structure Modeling: Overview and Assessment. , 2019, Annual review of biochemistry.

[3]  Linda Z. Shi,et al.  A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis in Caenorhabditis elegans , 2018, eLife.

[4]  M. Glotzer,et al.  Spatiotemporal Regulation of RhoA during Cytokinesis , 2018, Current Biology.

[5]  A. Echard,et al.  Membrane Traffic in the Late Steps of Cytokinesis , 2018, Current Biology.

[6]  S. Rusin,et al.  Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division , 2018, Current Biology.

[7]  G. Jensen,et al.  Coarse-grained simulations of actomyosin rings point to a nodeless model involving both unipolar and bipolar myosins , 2017, bioRxiv.

[8]  Davi R. Ortega,et al.  Structure of the fission yeast actomyosin ring during constriction , 2017, Proceedings of the National Academy of Sciences.

[9]  K. Oegema,et al.  TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis , 2017, bioRxiv.

[10]  M. Balasubramanian,et al.  Cell Polarity in Yeast. , 2017, Annual review of cell and developmental biology.

[11]  K. Gould,et al.  Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis , 2017, The Journal of cell biology.

[12]  T. Pollard.  Nine unanswered questions about cytokinesis , 2017, The Journal of cell biology.

[13]  M. Glotzer.  Cytokinesis in Metazoa and Fungi. , 2017, Cold Spring Harbor perspectives in biology.

[14]  B. O’Shaughnessy,et al.  A node organization in the actomyosin contractile ring generates tension and aids stability , 2017, Molecular biology of the cell.

[15]  K. Gould,et al.  Nanoscale architecture of the Schizosaccharomyces pombe contractile ring , 2017, eLife.

[16]  V. Minin,et al.  Myosin-independent cytokinesis in Giardia utilizes flagella to coordinate force generation and direct membrane trafficking , 2017, Proceedings of the National Academy of Sciences.

[17]  E. Bi,et al.  Mechanics and regulation of cytokinesis in budding yeast. , 2017, Seminars in cell & developmental biology.

[18]  T. Pollard,et al.  Analysis of interphase node proteins in fission yeast by quantitative and super resolution fluorescence microscopy , 2017, bioRxiv.

[19]  Yelena Zhuravlev,et al.  CYK-4 regulates Rac, but not Rho, during cytokinesis , 2017, Molecular biology of the cell.

[20]  D. Strickland,et al.  Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization , 2017, bioRxiv.

[21]  D. Loew,et al.  SIN-Dependent Dissociation of the SAD Kinase Cdr2 from the Cell Cortex Resets the Division Plane , 2017, Current Biology.

[22]  Patrick M. Irwin,et al.  The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis , 2017, Molecular biology of the cell.

[23]  S. West,et al.  Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis , 2016, Cell reports.

[24]  Jie Xiao,et al.  Redefining the roles of the FtsZ-ring in bacterial cytokinesis. , 2016, Current opinion in microbiology.

[25]  M. Osumi,et al.  A New Membrane Protein Sbg1 Links the Contractile Ring Apparatus and Septum Synthesis Machinery in Fission Yeast , 2016, PLoS genetics.

[26]  E. Paluch,et al.  The Actin Cortex: A Bridge between Cell Shape and Function. , 2016, Developmental cell.

[27]  T. Pollard,et al.  Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast , 2016, Proceedings of the National Academy of Sciences.

[28]  S. Grill,et al.  Cortical flow aligns actin filaments to form a furrow , 2016, eLife.

[29]  N. Burroughs,et al.  Actomyosin Ring Formation and Tension Generation in Eukaryotic Cytokinesis , 2016, Current Biology.

[30]  Anne Wald,et al.  Still and rotating myosin clusters determine cytokinetic ring constriction , 2016, Nature Communications.

[31]  T. Pollard,et al.  Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins , 2016, Nature Cell Biology.

[32]  G. Jürgens,et al.  Plant cytokinesis-No ring, no constriction but centrifugal construction of the partitioning membrane. , 2016, Seminars in cell & developmental biology.

[33]  P. Iglesias,et al.  Cytokinesis: Robust cell shape regulation. , 2016, Seminars in cell & developmental biology.

[34]  J. Dorn,et al.  A theoretical model of cytokinesis implicates feedback between membrane curvature and cytoskeletal organization in asymmetric cytokinetic furrowing , 2016, Molecular biology of the cell.

[35]  Jian-Qiu Wu,et al.  Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis , 2016, PLoS biology.

[36]  M. Glotzer,et al.  Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage , 2016, bioRxiv.

[37]  Franz Meitinger,et al.  Actomyosin ring driven cytokinesis in budding yeast , 2016, Seminars in cell & developmental biology.

[38]  K. Gould,et al.  Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis. , 2015, Developmental cell.

[39]  Brenton D Hoffman,et al.  Towards a Dynamic Understanding of Cadherin-Based Mechanobiology. , 2015, Trends in cell biology.

[40]  A. Mogilner,et al.  A Combination of Actin Treadmilling and Cross-Linking Drives Contraction of Random Actomyosin Arrays. , 2015, Biophysical journal.

[41]  T. Pollard,et al.  The fission yeast cytokinetic contractile ring regulates septum shape and closure , 2015, Journal of Cell Science.

[42]  V. Magidson,et al.  Morphogenesis of the Fission Yeast Cell through Cell Wall Expansion , 2015, Current Biology.

[43]  Donglei Zhang,et al.  The RhoGAP activity of CYK-4/MgcRacGAP functions non-canonically by promoting RhoA activation during cytokinesis , 2015, eLife.

[44]  K. Nakano,et al.  An actin–myosin-II interaction is involved in maintaining the contractile ring in fission yeast , 2015, Journal of Cell Science.

[45]  B. Baum,et al.  Kinetochore-localized PP1–Sds22 couples chromosome segregation to polar relaxation , 2015, Nature.

[46]  Sophie G. Martin,et al.  Pom1 gradient buffering through intermolecular auto-phosphorylation , 2015, Molecular systems biology.

[47]  Jian-Qiu Wu,et al.  Mechanistic insights into the anchorage of the contractile ring by anillin and Mid1. , 2015, Developmental cell.

[48]  Donglei Zhang,et al.  Aurora B kinase promotes cytokinesis by inducing centralspindlin oligomers that associate with the plasma membrane. , 2015, Developmental cell.

[49]  V. Simanis.  Pombe's thirteen – control of fission yeast cell division by the septation initiation network , 2015, Journal of Cell Science.

[50]  M. Petronczki,et al.  Cytokinesis in animal cells. , 2015, Cold Spring Harbor perspectives in biology.

[51]  S. Ishiwata,et al.  Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro , 2015, Nature Cell Biology.

[52]  John R. Allen,et al.  The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12 , 2015, The Journal of cell biology.

[53]  Mark Bathe,et al.  The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis , 2015, Molecular biology of the cell.

[54]  G. von Dassow,et al.  An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos , 2014, Molecular biology of the cell.

[55]  D. Gerlich,et al.  Cytokinetic abscission: molecular mechanisms and temporal control. , 2014, Developmental cell.

[56]  Dimitrios Vavylonis,et al.  Dynamic network morphology and tension buildup in a 3D model of cytokinetic ring assembly. , 2014, Biophysical journal.

[57]  Satoshi Okada,et al.  Architecture and dynamic remodeling of the septin cytoskeleton during the cell cycle , 2014, Nature Communications.

[58]  T. Mitchison,et al.  Spatial organization of cytokinesis signaling reconstituted in a cell-free system , 2014, Science.

[59]  M. Burkard,et al.  Centralspindlin assembly and 2 phosphorylations on MgcRacGAP by Polo-like kinase 1 initiate Ect2 binding in early cytokinesis , 2014, Cell cycle.

[60]  T. Pollard,et al.  Contractile ring stability in S. pombe depends on F-BAR protein Cdc15p and Bgs1p transport from the Golgi complex. , 2014, Cell reports.

[61]  Sophie G. Martin,et al.  Pom1 regulates the assembly of Cdr2–Mid1 cortical nodes for robust spatial control of cytokinesis , 2014, The Journal of cell biology.

[62]  Boris Guirao,et al.  Mechanism of cytokinetic contractile ring constriction in fission yeast. , 2014, Developmental cell.

[63]  E. Betzig,et al.  Nonmuscle Myosin II Isoforms Coassemble in Living Cells , 2014, Current Biology.

[64]  Sophie G. Martin,et al.  The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis , 2014, The Journal of cell biology.

[65]  T. Pollard,et al.  Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase , 2014, The Journal of cell biology.

[66]  Basile Audoly,et al.  Furrow constriction in animal cell cytokinesis. , 2014, Biophysical journal.

[67]  H. Kiyonari,et al.  Loss of a Rho-regulated actin nucleator, mDia2, impairs cytokinesis during mouse fetal erythropoiesis. , 2013, Cell reports.

[68]  Ivone M. Martins,et al.  Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction , 2013, The Journal of cell biology.

[69]  K. Oegema,et al.  A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during cytokinesis. , 2013, Developmental cell.

[70]  M. Balasubramanian,et al.  In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics , 2013, Nature Cell Biology.

[71]  H. Raghuraman,et al.  Binding of the CYK-4 Subunit of the Centralspindlin Complex Induces a Large Scale Conformational Change in the Kinesin Subunit , 2013, The Journal of Biological Chemistry.

[72]  S. Ramaswamy,et al.  The actin cortex as an active wetting layer , 2013, The European Physical Journal E.

[73]  E. Bi,et al.  Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast , 2013, The Journal of cell biology.

[74]  L. Collinson,et al.  Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis , 2012, Nature.

[75]  W. Earnshaw,et al.  The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis , 2012, Nature Reviews Molecular Cell Biology.

[76]  J. Labbé,et al.  RhoA activation during polarization and cytokinesis of the early Caenorhabditis elegans embryo is differentially dependent on NOP-1 and CYK-4 , 2012, Molecular biology of the cell.

[77]  Sergio A. Rincon,et al.  Mid1/anillin and the spatial regulation of cytokinesis in fission yeast , 2012, Cytoskeleton.

[78]  Patrick M. McCall,et al.  Self-organization of myosin II in reconstituted actomyosin bundles. , 2012, Biophysical journal.

[79]  A. Boudaoud,et al.  Contributions of Turgor Pressure, the Contractile Ring, and Septum Assembly to Forces in Cytokinesis in Fission Yeast , 2012, Current Biology.

[80]  M. Rao,et al.  Cylindrical cellular geometry ensures fidelity of division site placement in fission yeast , 2012, Journal of Cell Science.

[81]  Nurhan Özlü,et al.  Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis , 2012, Molecular biology of the cell.

[82]  Jay R. Unruh,et al.  Actin depolymerization drives actomyosin ring contraction during budding yeast cytokinesis. , 2012, Developmental cell.

[83]  Pablo A. Iglesias,et al.  Deconvolution of the Cellular Force-Generating Subsystems that Govern Cytokinesis Furrow Ingression , 2012, PLoS Comput. Biol..

[84]  J. Sellers,et al.  Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis , 2012, Proceedings of the National Academy of Sciences.

[85]  Martin Lenz,et al.  Contractile units in disordered actomyosin bundles arise from F-actin buckling. , 2012, Physical review letters.

[86]  Kuan-Chung Su,et al.  Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis. , 2011, Developmental cell.

[87]  Gregory Jedd,et al.  Myosin concentration underlies cell size–dependent scalability of actomyosin ring constriction , 2011, The Journal of cell biology.

[88]  T. Pollard,et al.  Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring , 2011, The Journal of cell biology.

[89]  J. Tinevez,et al.  Polar actomyosin contractility destabilizes the position of the cytokinetic furrow , 2011, Nature.

[90]  K. Gould,et al.  Temporal Control of Contractile Ring Assembly by Plo1 Regulation of Myosin II Recruitment by Mid1/Anillin , 2011, Current Biology.

[91]  Ivo A. Telley,et al.  A Minimal Midzone Protein Module Controls Formation and Length of Antiparallel Microtubule Overlaps , 2010, Cell.

[92]  Christopher P. Arthur,et al.  Insights into Antiparallel Microtubule Crosslinking by PRC1, a Conserved Nonmotor Microtubule Binding Protein , 2010, Cell.

[93]  M. Lord,et al.  Tropomyosin and Myosin-II Cellular Levels Promote Actomyosin Ring Assembly in Fission Yeast , 2010, Molecular biology of the cell.

[94]  M. Glotzer,et al.  Clustering of Centralspindlin Is Essential for Its Accumulation to the Central Spindle and the Midbody , 2009, Current Biology.

[95]  G. von Dassow,et al.  Action at a distance during cytokinesis , 2009, The Journal of cell biology.

[96]  J. Tinevez,et al.  Role of cortical tension in bleb growth , 2009, Proceedings of the National Academy of Sciences.

[97]  D. Drubin,et al.  Isolation and partial purification of the Saccharomyces cerevisiae cytokinetic apparatus , 2009, Cytoskeleton.

[98]  Paul Nurse,et al.  A spatial gradient coordinates cell size and mitotic entry in fission yeast , 2009, Nature.

[99]  Sophie G. Martin,et al.  Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle , 2009, Nature.

[100]  P. Nurse,et al.  Spatial Control of Cytokinesis by Cdr2 Kinase and Mid1/Anillin Nuclear Export , 2009, Current Biology.

[101]  Karen Oegema,et al.  Structural Memory in the Contractile Ring Makes the Duration of Cytokinesis Independent of Cell Size , 2009, Cell.

[102]  J. Pringle,et al.  The anaphase-promoting complex promotes actomyosin-ring disassembly during cytokinesis in yeast. , 2009, Molecular biology of the cell.

[103]  Ann L. Miller,et al.  Regulation of cytokinesis by Rho GTPase flux , 2008, Nature Cell Biology.

[104]  K. Oegema,et al.  Inhibition of Rac by the GAP Activity of Centralspindlin Is Essential for Cytokinesis , 2008, Science.

[105]  S. Narumiya,et al.  mDia2 induces the actin scaffold for the contractile ring and stabilizes its position during cytokinesis in NIH 3T3 cells. , 2008, Molecular biology of the cell.

[106]  Andrew B Goryachev,et al.  Dynamics of Cdc42 network embodies a Turing‐type mechanism of yeast cell polarity , 2008, FEBS letters.

[107]  Pablo A. Iglesias,et al.  Interactions between Myosin and Actin Crosslinkers Control Cytokinesis Contractility Dynamics and Mechanics , 2008, Current Biology.

[108]  Dimitrios Vavylonis,et al.  Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast , 2008, Science.

[109]  A. Spang,et al.  Functions of the novel RhoGAP proteins RGA-3 and RGA-4 in the germ line and in the early embryo of C. elegans , 2007, Development.

[110]  Florian Odronitz,et al.  Drawing the tree of eukaryotic life based on the analysis of 2,269 manually annotated myosins from 328 species , 2007, Genome Biology.

[111]  A. Hyman,et al.  The Rho GTPase-activating proteins RGA-3 and RGA-4 are required to set the initial size of PAR domains in Caenorhabditis elegans one-cell embryos , 2007, Proceedings of the National Academy of Sciences.

[112]  Masako Osumi,et al.  Three-dimensional arrangement of F-actin in the contractile ring of fission yeast , 2007, The Journal of cell biology.

[113]  Anthony A. Hyman,et al.  Supporting Material : Stress Generation and Filament Turnover During Actin Ring Constriction , 2007 .

[114]  M. Osumi,et al.  The (1,3)β‐d‐glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation , 2007, Molecular microbiology.

[115]  M. Balasubramanian,et al.  Polarity determinants Tea1p, Tea4p, and Pom1p inhibit division-septum assembly at cell ends in fission yeast. , 2007, Developmental cell.

[116]  Sophie G. Martin,et al.  The Cell-End Factor Pom1p Inhibits Mid1p in Specification of the Cell Division Plane in Fission Yeast , 2006, Current Biology.

[117]  J. R. Daum,et al.  The reversibility of mitotic exit in vertebrate cells , 2006, Nature.

[118]  T. Veenstra,et al.  Cytokinesis regulator ECT2 changes its conformation through phosphorylation at Thr-341 in G2/M phase , 2006, Oncogene.

[119]  S. Yonemura,et al.  Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis , 2006, Journal of Cell Science.

[120]  T. Pollard,et al.  Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast. , 2005, Molecular biology of the cell.

[121]  Kyung S. Lee,et al.  Inhibition of Cyclin-dependent Kinase 1 Induces Cytokinesis without Chromosome Segregation in an ECT2 and MgcRacGAP-dependent Manner* , 2005, Journal of Biological Chemistry.

[122]  Thomas D Pollard,et al.  Counting Cytokinesis Proteins Globally and Locally in Fission Yeast , 2005, Science.

[123]  Nico Stuurman,et al.  Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[124]  M. Glotzer,et al.  An ECT2–centralspindlin complex regulates the localization and function of RhoA , 2005, The Journal of cell biology.

[125]  Francesco S. Pavone,et al.  Nuclear and Division-Plane Positioning Revealed by Optical Micromanipulation , 2005, Current Biology.

[126]  G. von Dassow,et al.  A microtubule-dependent zone of active RhoA during cleavage plane specification , 2005, The Journal of cell biology.

[127]  F. Matsumura.  Regulation of myosin II during cytokinesis in higher eukaryotes. , 2005, Trends in cell biology.

[128]  Fred Chang,et al.  Dynamic positioning of the fission yeast cell division plane. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[129]  D. Robinson,et al.  Balance of actively generated contractile and resistive forces controls cytokinesis dynamics. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[130]  Junjie Chen,et al.  The Tandem BRCT Domains of Ect2 Are Required for Both Negative and Positive Regulation of Ect2 in Cytokinesis* , 2005, Journal of Biological Chemistry.

[131]  Nicole Bordes,et al.  C-Terminal Anchoring of mid1p to Membranes Stabilizes Cytokinetic Ring Position in Early Mitosis in Fission Yeast , 2004, Molecular and Cellular Biology.

[132]  T. Pollard,et al.  UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II , 2004, The Journal of cell biology.

[133]  K. Verbrugghe,et al.  SPD-1 Is Required for the Formation of the Spindle Midzone but Is Not Essential for the Completion of Cytokinesis in C. elegans Embryos , 2004, Current Biology.

[134]  E. Nigg,et al.  Cell cycle regulation of central spindle assembly , 2004, Nature.

[135]  Thomas D Pollard,et al.  Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. , 2003, Developmental cell.

[136]  R. Vale,et al.  Molecular requirements for actin-based lamella formation in Drosophila S2 cells , 2003, The Journal of cell biology.

[137]  T. Pollard,et al.  The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin , 2003, The Journal of cell biology.

[138]  Timothy J Mitchison,et al.  Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor , 2003, Science.

[139]  Guy Cavet,et al.  Quantitation of the distribution and flux of myosin-II during cytokinesis , 2002, BMC Cell Biology.

[140]  F. Lottspeich,et al.  Recruitment of cortexillin into the cleavage furrow is controlled by Rac1 and IQGAP‐related proteins , 2001, The EMBO journal.

[141]  S. Yumura.  Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells , 2001, The Journal of cell biology.

[142]  V. Doye,et al.  A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing , 2001, The Journal of cell biology.

[143]  R. Pelham,et al.  Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombe , 2001, Nature Cell Biology.

[144]  F. Chang,et al.  Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeast. , 2000, Molecular biology of the cell.

[145]  S. Narumiya,et al.  Accumulation of GTP-bound RhoA during Cytokinesis and a Critical Role of ECT2 in This Accumulation* , 2000, The Journal of Biological Chemistry.

[146]  E. Salmon,et al.  The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle , 2000, Current Biology.

[147]  T. Miki,et al.  Human Ect2 Is an Exchange Factor for Rho Gtpases, Phosphorylated in G2/M Phases, and Involved in Cytokinesis , 1999, The Journal of cell biology.

[148]  H. Bellen,et al.  A putative exchange factor for Rho1 GTPase is required for initiation of cytokinesis in Drosophila. , 1999, Genes & development.

[149]  Daniel J. Lew,et al.  Involvement of an Actomyosin Contractile Ring in Saccharomyces cerevisiae Cytokinesis , 1998, The Journal of cell biology.

[150]  H. Schnabel,et al.  cyk-1: a C. elegans FH gene required for a late step in embryonic cytokinesis. , 1998, Journal of cell science.

[151]  B. Jégou,et al.  MgcRacGAP, A New Human GTPase-activating Protein for Rac and Cdc42 Similar to Drosophila rotundRacGAP Gene Product, Is Expressed in Male Germ Cells* , 1998, The Journal of Biological Chemistry.

[152]  Masayuki Yamamoto,et al.  Type II Myosin Heavy Chain Encoded by the myo2 Gene Composes the Contractile Ring during Cytokinesis in Schizosaccharomyces pombe , 1997, The Journal of cell biology.

[153]  M. Sohrmann,et al.  The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast. , 1996, Genes & development.

[154]  Y. Wang,et al.  Signals from the spindle midzone are required for the stimulation of cytokinesis in cultured epithelial cells. , 1996, Molecular biology of the cell.

[155]  R. T. Tregear,et al.  Movement and force produced by a single myosin head , 1995, Nature.

[156]  S. Wasserman,et al.  Diaphanous is required for cytokinesis in Drosophila and shares domains of similarity with the products of the limb deformity gene. , 1994, Development.

[157]  T. Pollard,et al.  Differential localization of myosin-II isozymes in human cultured cells and blood cells. , 1994, Journal of cell science.

[158]  Corey Nislow,et al.  A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles , 1992, Nature.

[159]  T. E. Schroeder,et al.  Association of Actin and Myosin in the Contractile Ring a , 1990, Annals of the New York Academy of Sciences.

[160]  J. Spudich,et al.  Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. , 1987, Science.

[161]  Yoshio Fukui,et al.  Reversible cyclic AMP-dependent change in distribution of myosin thick filaments in Dictyostelium , 1985, Nature.

[162]  J. Sanger,et al.  Banding and polarity of actin filaments in interphase and cleaving cells , 1980, The Journal of cell biology.

[163]  I. Mabuchi,et al.  The effect of myosin antibody on the division of starfish blastomeres , 1977, The Journal of cell biology.

[164]  T. Pollard,et al.  Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells , 1976, The Journal of cell biology.

[165]  T. E. Schroeder,et al.  Actin in dividing cells: contractile ring filaments bind heavy meromyosin. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[166]  K. Dan,et al.  Tension at the surface of the dividing sea-urchin egg. , 1972, The Journal of experimental biology.

[167]  T. E. Schroeder.  The contractile ring. II. Determining its brief existence, volumetric changes, and vital role in cleaving Arbacia eggs. , 1972 .

[168]  R. Rappaport,et al.  Cell Division: Direct Measurement of Maximum Tension Exerted by Furrow of Echinoderm Eggs , 1967, Science.

[169]  Y. Hiramoto.  Observations and measurements of sea urchin eggs with a centrifuge microscope , 1967, Journal of the American Veterinary Medical Association.

[170]  Yu-Li Wang,et al.  Distinct pathways for the early recruitment of myosin II and actin to the cytokinetic furrow. , 2008, Molecular biology of the cell.

[171]  R. Saint,et al.  A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. , 2003, Developmental cell.

[172]  M. Glotzer,et al.  Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. , 2002, Developmental cell.

[173]  P. Nurse,et al.  Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring. , 1996, Journal of cell science.

[174]  T. Pollard,et al.  Arrangement of actin filaments and myosin-like filaments in the contractile ring and of actin-like filaments in the mitotic spindle of dividing HeLa cells. , 1986, Journal of ultrastructure and molecular structure research.

[175]  J. Hyams,et al.  Localization of F-actin through the cell division cycle of Schizosaccharomyces pombe. , 1985 .

[176]  Y. Hiramoto,et al.  FORCE EXERTED BY THE CLEAVAGE FURROW OF SEA URCHIN EGGS , 1975, Development, growth & differentiation.

[177]  Y. Hiramoto,et al.  Rheological properties of sea urchin eggs. , 1970, Biorheology.

[178]  T. E. Schroeder.  The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. , 1970, Zeitschrift fur Zellforschung und mikroskopische Anatomie.