Chlorophyll Fluorescence Imaging of the Chlorophyll Fluorescence Induction Phenomenon for Plant Health Monitoring

Chlorophyll fluorescence imaging is useful as a non-destructive method for evaluating photosynthetic function of plants. A dynamic change in chlorophyll fluorescence intensity, known as the chlorophyll fluorescence induction phenomenon, can be observed by illuminating a dark-adapted green leaf with a stable intensity excitation light. The time course of the chlorophyll fluorescence intensity during this phenomenon, presented as an induction curve, varies depending on the health of the plant. Imaging of the chlorophyll fluorescence induction phenomenon may be used to monitor the health status of plants. In this review, we introduce studies on our chlorophyll fluorescence imaging system for plant health monitoring of tomato crops grown in a greenhouse. We first developed a prototype of the imaging system to confirm performance on detection of artificially induced light stress in a single leaf and whole plant. Based on the successful detection of photosynthetic dysfunction caused by light stress using the prototype, we applied our chlorophyll fluorescence imaging system to measurements of the chlorophyll fluorescence induction curves of tomato crops grown in a semi-commercial greenhouse. Upon comparing the induction curves of 20 tomato crops planted on a north-south lane in the greenhouse, we found two plants with unique induction curve patterns. One of these two plants showed visible symptoms of physiological dysfunction 7 days after measurement. Thus, our chlorophyll fluorescence imaging system appears to be a useful tool for plant health monitoring in horticultural production.

[1]  G. Krause,et al.  Chlorophyll Fluorescence and Photosynthesis: The Basics , 1991 .

[2]  Hiroshige Nishina,et al.  Early Detection of Water Stress in Tomato Plants Based on Projected Plant Area , 2007 .

[3]  B. Genty.  Quantitative mapping of leaf photosyn-thesis using chlorophyll fluorescence imaging , 1994 .

[4]  H. Nishina,et al.  Chlorophyll Fluorescence Imaging at 77 K for Assessing the Heterogeneously Distributed Light Stress Over a Leaf Surface , 2007 .

[5]  K Raschke,et al.  Topography of photosynthetic activity of leaves obtained from video images of chlorophyll fluorescence. , 1989, Plant physiology.

[6]  K. Kurata,et al.  WATER STRESS ESTIMATION OF TOMATO CANOPY BASED ON MACHINE VISION , 1996 .

[7]  Y. Hashimoto,et al.  COMPUTER CONTROL OF SHORT TERM PLANT GROWTH BY MONITORING LEAF TEMPERATURE , 1980 .

[8]  K. Omasa,et al.  Diagnosis of Invisible Photosynthetic Injury Caused by a Herbicide (Basta) with Chlorophyll Fluorescence Imaging System , 2003 .

[9]  K Maxwell,et al.  Chlorophyll fluorescence--a practical guide. , 2000, Journal of experimental botany.

[10]  K. Omasa,et al.  Simultaneous measurement of stomatal conductance, non-photochemical quenching, and photochemical yield of photosystem II in intact leaves by thermal and chlorophyll fluorescence imaging. , 2003, Plant & cell physiology.

[11]  Wolfgang Bilger,et al.  Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis , 1990, Photosynthesis Research.

[12]  Y. Hashimoto.  RECENT STRATEGIES OF OPTIMAL GROWTH REGULATION BY THE SPEAKING PLANT CONCEPT , 1989 .

[13]  Paul F. Daley,et al.  Chlorophyll fluorescence analysis and imaging in plant stress and disease , 1994 .

[14]  Seiichi Arima,et al.  Early diagnosis of water stress in tomato plant by monitoring of projected area with digital still camera. , 2009 .

[15]  E. Govindje,et al.  Sixty-Three Years Since Kautsky: Chlorophyll a Fluorescence , 1995 .

[16]  H. Nishina,et al.  DETECTION OF WATER STRESS INDUCED PHOTOSYNTHETIC DYSFUNCTION IN TOMATO PLANT LEAF WITH PRI MEASUREMENT , 2008 .

[17]  Hiroshige Nishina,et al.  Chlorophyll fluorescence imaging as a plant diagnosis tool in protected horticulture , 2008 .

[18]  J. Briantais,et al.  The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence , 1989 .

[19]  Ilkka Leinonen,et al.  Thermal Imaging for the Study of Plant Water Relations , 2003 .

[20]  M. Onoe,et al.  Image analysis of chlorophyll fluorescence transients for diagnosing the photosynthetic system of attached leaves. , 1987, Plant physiology.

[21]  Francine Heisel,et al.  Detection of vegetation stress via a new high resolution fluorescence imaging system , 1996 .

[22]  K. Niyogi,et al.  Non-photochemical quenching. A response to excess light energy. , 2001, Plant physiology.