End‐binding protein 1 (EB1) up‐regulation is an early event in colorectal carcinogenesis

End‐binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa (“field effect”) of human and animal tissue. We also performed siRNA‐knockdown in colon cancer cell lines. EB1 is up‐regulated in early and field carcinogenesis in the colon, and the cellular/nano‐architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.

[1]  Yukiko Nakamura,et al.  Proteomic-based identification of the APC-binding protein EB1 as a candidate of novel tissue biomarker and therapeutic target for colorectal cancer. , 2012, Journal of proteomics.

[2]  E. Triphosphat,et al.  FEBS Letters , 1987, FEBS Letters.

[3]  J. Arends,et al.  Morphological changes in mesothelial cells induced by shed menstrual endometrium in vitro are not primarily due to apoptosis or necrosis. , 2000, Human reproduction.

[4]  Inke Näthke,et al.  Cytoskeleton out of the cupboard: colon cancer and cytoskeletal changes induced by loss of APC , 2006, Nature Reviews Cancer.

[5]  J. Palazzo,et al.  Reduced expression of molecules of the cadherin/catenin complex in the transition from colorectal adenoma to carcinoma. , 1997, Anticancer research.

[6]  J. Watari,et al.  Clinicopathologic implications of genetic instability in intestinal-type gastric cancer and intestinal metaplasia as a precancerous lesion: proof of field cancerization in the stomach. , 2008, American journal of clinical pathology.

[7]  Y. Shen,et al.  p53-dependent apoptosis pathways. , 2001, Advances in cancer research.

[8]  Young L. Kim,et al.  Optical Markers in Duodenal Mucosa Predict the Presence of Pancreatic Cancer , 2007, Clinical Cancer Research.

[9]  Hongxia Zhu,et al.  EB1 acts as an oncogene via activating β‐catenin/TCF pathway to promote cellular growth and inhibit apoptosis , 2009, Molecular Carcinogenesis.

[10]  J. Behrens,et al.  Cadherins and Catenins: Role in Signal Transduction and Tumor Progression , 2004, Cancer and Metastasis Reviews.

[11]  W. Hong,et al.  Increased polysomies of chromosomes 7 and 17 during head and neck multistage tumorigenesis. , 1993, Cancer research.

[12]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[13]  M. Adams,et al.  Examination of actin and microtubule dependent APC localisations in living mammalian cells , 2006, BMC Cell Biology.

[14]  折茂 達也.  Proteomic profiling reveals the prognostic value of adenomatous polyposis coli-end-binding protein 1 in hepatocellular carcinoma , 2009 .

[15]  Vadim Backman,et al.  Risk Stratification of Colon Carcinogenesis through Enhanced Backscattering Spectroscopy Analysis of the Uninvolved Colonic Mucosa , 2006, Clinical Cancer Research.

[16]  Vadim Backman,et al.  Investigating Population Risk Factors of Pancreatic Cancer by Evaluation of Optical Markers in the Duodenal Mucosa , 2009, Disease markers.

[17]  J. Amos-Landgraf,et al.  A target-selected Apc-mutant rat kindred enhances the modeling of familial human colon cancer , 2007, Proceedings of the National Academy of Sciences.

[18]  R. Brent,et al.  APC binds to the novel protein EB1. , 1995, Cancer research.

[19]  D. Pellman,et al.  The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Ashish K. Tiwari,et al.  Biological Mechanisms Underlying Structural Changes Induced by Colorectal Field Carcinogenesis Measured with Low-Coherence Enhanced Backscattering (LEBS) Spectroscopy , 2013, PloS one.

[21]  Dina Dikovskaya,et al.  Lack of adenomatous polyposis coli protein correlates with a decrease in cell migration and overall changes in microtubule stability. , 2007, Molecular biology of the cell.

[22]  S. Dedhar,et al.  Dysregulation of integrin-linked kinase (ILK) signaling in colonic polyposis , 2001, Oncogene.

[23]  Bert Vogelstein,et al.  APC mutations occur early during colorectal tumorigenesis , 1992, Nature.

[24]  R. Fodde,et al.  The role of the APC tumor suppressor in chromosomal instability. , 2006, Genome dynamics.

[25]  Xiaobo Zhou,et al.  Overexpression of EB1 in human esophageal squamous cell carcinoma (ESCC) may promote cellular growth by activating β-catenin/TCF pathway , 2005, Oncogene.

[26]  P. Quirke,et al.  Experimental models of colorectal cancer , 1998, Diseases of the colon and rectum.

[27]  S. Altschul,et al.  Identification of FAP locus genes from chromosome 5q21. , 1991, Science.

[28]  Vadim Backman,et al.  Low-coherent backscattering spectroscopy for tissue characterization. , 2005, Applied optics.

[29]  Vadim Backman,et al.  Light-scattering technologies for field carcinogenesis detection: a modality for endoscopic prescreening. , 2011, Gastroenterology.

[30]  Li Fu,et al.  Oncogenic function of microtubule end‐binding protein 1 in breast cancer , 2010, The Journal of pathology.

[31]  A. Ben-Ze'ev,et al.  β‐Catenin signaling in biological control and cancer , 2007 .

[32]  R. Nishigaki,et al.  Proteomic identification of differentially‐expressed genes in human gastric carcinomas , 2005, Proteomics.

[33]  Vadim Backman,et al.  Association between rectal optical signatures and colonic neoplasia: potential applications for screening. , 2009, Cancer research.

[34]  A. Ben-Ze'ev,et al.  beta-Catenin signaling in biological control and cancer. , 2007, Journal of cellular biochemistry.

[35]  P. Rougier,et al.  Absence of somatic alterations of the EB1 gene adenomatous polyposis coli-associated protein in human sporadic colorectal cancers. , 1998, British Journal of Cancer.

[36]  J. Jimenez-Heffernan,et al.  Peritoneal dialysis and epithelial-to-mesenchymal transition of mesothelial cells. , 2003, The New England journal of medicine.

[37]  Alan Hall,et al.  The cytoskeleton and cancer , 2009, Cancer and Metastasis Reviews.

[38]  B. Bierer,et al.  Eb1 Proteins Regulate Microtubule Dynamics, Cell Polarity, and Chromosome Stability , 2000, The Journal of cell biology.

[39]  Rebecca A Green,et al.  APC and EB1 function together in mitosis to regulate spindle dynamics and chromosome alignment. , 2005, Molecular biology of the cell.

[40]  Tan Zhang,et al.  Microtubule plus-end binding protein EB1 is necessary for muscle cell differentiation, elongation and fusion , 2009, Journal of Cell Science.

[41]  Yi-Song Wang,et al.  WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. , 1994, Cancer research.

[42]  C. R. Leemans,et al.  A genetic explanation of Slaughter's concept of field cancerization: evidence and clinical implications. , 2003, Cancer research.