US-Like Strain of Porcine Epidemic Diarrhea Virus Outbreaks in Taiwan, 2013–2014

ABSTRACT Since late 2013, several outbreaks of porcine epidemic diarrhea virus (PEDV) infection have emerged in Taiwan. Suckling piglets under 2 weeks of age showed severe vomiting and watery yellowish diarrhea with morbidity and mortality ranging from 80 to 100% and 90 to 100%, respectively. A total of 68 samples from 25 pig farms were confirmed as positive for PEDV and negative for rotavirus and transmissible gastroenteritis virus by reverse transcription PCR, and the partial S gene of PEDV was analyzed. Phylogenetic analysis places all 18 Taiwanese PEDV isolates collected during this outbreak in the same clade as the US strains of PEDV. This novel PEDV is prevailing and currently causing severe outbreaks in Taiwan.

[1]  K. Schwartz,et al.  Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences , 2013, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[2]  D. Song,et al.  Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines , 2012, Virus Genes.

[3]  Shibo Jiang,et al.  The spike protein of SARS-CoV — a target for vaccine and therapeutic development , 2009, Nature Reviews Microbiology.

[4]  Gavin J. D. Smith,et al.  Evolutionary Insights into the Ecology of Coronaviruses , 2007, Journal of Virology.

[5]  Y. Jang,et al.  Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus. , 2002, Molecules and cells.

[6]  H. Hotzel,et al.  Nested reverse transcriptase-polymerase chain reaction for the detection of group A rotaviruses. , 2002, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[7]  D. Song,et al.  Differential Detection of Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus by Duplex RT-PCR , 2001, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[8]  Marian C. Horzinek,et al.  Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR , 1995, Journal of clinical microbiology.