Rapid and High‐Throughput Forensic Short Tandem Repeat Typing Using a 96‐Lane Microfabricated Capillary Array Electrophoresis Microdevice *

ABSTRACT: A 96‐channel microfabricated capillary array electrophoresis (μCAE) device was evaluated for forensic short tandem repeat (STR) typing using PowerPlex 16® and AmpFℓSTR® Profiler Plus® multiplex PCR systems. The high‐throughput μCAE system produced high‐speed <30‐min parallel sample separations with single‐base resolution. Forty‐eight previously analyzed single‐source samples were accurately typed, as confirmed on an ABI Prism 310 and/or the Hitachi FMBIO II. Minor alleles in 3:1 mixture samples containing female and male DNA were reliably typed as well. The instrument produced full profiles from sample DNA down to 0.17 ng, a threshold similar to that found for the ABI 310. Seventeen nonprobative samples from various evidentiary biological stains were also correctly typed. The successful application of the μCAE device to actual forensic STR typing samples is a significant step toward the development of a completely integrated STR analysis microdevice.

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