Basic research and clinical applications of non-hematopoietic stem cells, 4-5 April 2008, Tubingen, Germany.

From 4 to 5 April 2008, international experts met for the second time in Tubingen, Germany, to present and discuss the latest proceedings in research on non-hematopoietic stem cells (NHSC). This report presents issues of basic research including characterization, isolation, good manufacturing practice (GMP)-like production and imaging as well as clinical applications focusing on the regenerative and immunomodulatory capacities of NHSC.

[1]  M. Bonin,et al.  Generation of pluripotent stem cells from adult human testis , 2008, Nature.

[2]  A. Reinisch,et al.  Rapid large-scale expansion of functional mesenchymal stem cells from unmanipulated bone marrow without animal serum. , 2008, Tissue engineering. Part C, Methods.

[3]  K. Hirschi,et al.  Assessing identity, phenotype, and fate of endothelial progenitor cells. , 2008, Arteriosclerosis, thrombosis, and vascular biology.

[4]  E. Fiumana,et al.  Local Activation or Implantation of Cardiac Progenitor Cells Rescues Scarred Infarcted Myocardium Improving Cardiac Function , 2008, Circulation research.

[5]  Mark A Sussman,et al.  Myocardial Induction of Nucleostemin in Response to Postnatal Growth and Pathological Challenge , 2008, Circulation research.

[6]  G. Dini,et al.  Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study , 2008, The Lancet.

[7]  A. Sbarbati,et al.  Mesenchymal stem cells share molecular signature with mesenchymal tumor cells and favor early tumor growth in syngeneic mice , 2008, Oncogene.

[8]  P. Conte,et al.  Donor cell-derived osteopoiesis originates from a self-renewing stem cell with a limited regenerative contribution after transplantation. , 2008, Blood.

[9]  T. Billiar,et al.  HMGB1: Endogenous Danger Signaling , 2008, Molecular medicine.

[10]  C. Bearzi,et al.  Activation of Cardiac Progenitor Cells Reverses the Failing Heart Senescent Phenotype and Prolongs Lifespan , 2008, Circulation research.

[11]  D. Kraitchman,et al.  Imaging of stem cells using MRI , 2008, Basic Research in Cardiology.

[12]  F. Ris,et al.  Clinical Magnetic Resonance Imaging of Pancreatic Islet Grafts After Iron Nanoparticle Labeling , 2008, American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons.

[13]  C. Bearzi,et al.  Formation of large coronary arteries by cardiac progenitor cells , 2008, Proceedings of the National Academy of Sciences.

[14]  G. Seitz,et al.  Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells , 2008, Cell proliferation.

[15]  S. Cherry,et al.  Radiolabeling Rhesus Monkey CD34+ Hematopoietic and Mesenchymal Stem Cells with 64Cu-Pyruvaldehyde-Bis(N4-Methylthiosemicarbazone) for MicroPET Imaging , 2008, Molecular imaging.

[16]  K. Landfester,et al.  Carboxylated Superparamagnetic Iron Oxide Particles Label Cells Intracellularly Without Transfection Agents , 2008, Molecular Imaging and Biology.

[17]  W. Woodward,et al.  Tumor irradiation increases the recruitment of circulating mesenchymal stem cells into the tumor microenvironment. , 2007, Cancer research.

[18]  M. Santini,et al.  Identification of Myocardial and Vascular Precursor Cells in Human and Mouse Epicardium , 2007, Circulation research.

[19]  S. Majewski,et al.  Imaging of Mesenchymal Stem Cell Transplant by Bioluminescence and PET , 2007, Journal of Nuclear Medicine.

[20]  Ross Tubo,et al.  Mesenchymal stem cells within tumour stroma promote breast cancer metastasis , 2007, Nature.

[21]  Franco Locatelli,et al.  Cotransplantation of ex vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem-cell transplantation. , 2007, Blood.

[22]  C D Claussen,et al.  Aptamer-based isolation and subsequent imaging of mesenchymal stem cells in ischemic myocard by magnetic resonance imaging. , 2007, RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin.

[23]  W. Friedrich,et al.  Human multipotent mesenchymal stromal cells inhibit proliferation of PBMCs independently of IFNgammaR1 signaling and IDO expression. , 2007, Blood.

[24]  O. Ringdén,et al.  No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients , 2007, Haematologica.

[25]  S. Conrad,et al.  „Tissue engineering“ und Stammzellforschung in der Urologie für den rekonstruktiven bzw. regenerativen Therapieansatz , 2007, Der Urologe.

[26]  A. Luttun,et al.  Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity , 2007, Genome Biology.

[27]  J. Ryan,et al.  Interferon‐γ does not break, but promotes the immunosuppressive capacity of adult human mesenchymal stem cells , 2007 .

[28]  C. Claussen,et al.  Transferrin receptor upregulation: in vitro labeling of rat mesenchymal stem cells with superparamagnetic iron oxide. , 2007, Radiology.

[29]  Karl Kashofer,et al.  Human platelet lysate can replace fetal bovine serum for clinical‐scale expansion of functional mesenchymal stromal cells , 2007, Transfusion.

[30]  L. Fouillard,et al.  Immunosuppressive Effects of Mesenchymal Stem Cells: Involvement of HLA-G , 2007, Transplantation.

[31]  Andreas Reinisch,et al.  Humanized system to propagate cord blood-derived multipotent mesenchymal stromal cells for clinical application. , 2007, Regenerative medicine.

[32]  A. Reinisch,et al.  Immune Cells Mimic the Morphology of Endothelial Progenitor Colonies In Vitro , 2007, Stem cells.

[33]  L. Kanz,et al.  Novel Markers for the Prospective Isolation of Human MSC , 2007, Annals of the New York Academy of Sciences.

[34]  P. Ljungman,et al.  Transplantation of mesenchymal stem cells to enhance engraftment of hematopoietic stem cells , 2007, Leukemia.

[35]  E. Horwitz,et al.  Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs. , 2007, Blood.

[36]  H. Klüter,et al.  Human AB Serum and Thrombin‐Activated Platelet‐Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue , 2007, Stem cells.

[37]  J. Bulte,et al.  Developing MR reporter genes: promises and pitfalls , 2007, NMR in biomedicine.

[38]  G. Duda,et al.  Mesenchymal Stem Cells Regulate Angiogenesis According to Their Mechanical Environment , 2007, Stem cells.

[39]  Lothar Just,et al.  Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation. , 2007, Differentiation; research in biological diversity.

[40]  C. Bearzi,et al.  Concise Review: Stem Cells, Myocardial Regeneration, and Methodological Artifacts , 2007, Stem cells.

[41]  J. Prchal,et al.  Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals. , 2007, Blood.

[42]  P. Anversa,et al.  If I can stop one heart from breaking. , 2007, Circulation.

[43]  E. Chavakis,et al.  High-Mobility Group Box 1 Activates Integrin-Dependent Homing of Endothelial Progenitor Cells , 2007, Circulation research.

[44]  E. Lam,et al.  Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth , 2007, Leukemia.

[45]  Piotr Walczak,et al.  Artificial reporter gene providing MRI contrast based on proton exchange , 2007, Nature Biotechnology.

[46]  E. Lam,et al.  Mesenchymal Stem Cells Inhibit Dendritic Cell Differentiation and Function by Preventing Entry Into the Cell Cycle , 2007, Transplantation.

[47]  S. Houser,et al.  Pim-1 regulates cardiomyocyte survival downstream of Akt , 2006, Nature Medicine.

[48]  C. Bearzi,et al.  Human cardiac stem cells , 2005, Proceedings of the National Academy of Sciences.

[49]  T. Asahara.  Cell therapy and gene therapy using endothelial progenitor cells for vascular regeneration. , 2007, Handbook of experimental pharmacology.

[50]  J. Wiskirchen,et al.  Progress in characterization, preparation and clinical applications of non-hematopoietic stem cells, 29-30 September 2006, Tübingen, Germany. , 2007, Cytotherapy.

[51]  L. Zangi,et al.  High-yield isolation, expansion, and differentiation of murine bone marrow-derived mesenchymal stem cells using fibrin microbeads (FMB). , 2007, Cloning and stem cells.

[52]  H. Masuda,et al.  Endothelial progenitor cells for postnatal vasculogenesis , 2007, Clinical and Experimental Nephrology.

[53]  E. Seifried,et al.  Mesenchymal stem cells display coordinated rolling and adhesion behavior on endothelial cells. , 2006, Blood.

[54]  Liangfu Zhou,et al.  Tracking neural stem cells in patients with brain trauma. , 2006, The New England journal of medicine.

[55]  Jianyi(Jay) Zhang,et al.  Multipotent Adult Progenitor Cells from Swine Bone Marrow , 2006, Stem cells.

[56]  G. Bergers,et al.  The bone marrow constitutes a reservoir of pericyte progenitors , 2006, Journal of leukocyte biology.

[57]  Mark A Sussman,et al.  Nuclear targeting of Akt antagonizes aspects of cardiomyocyte hypertrophy , 2006, Proceedings of the National Academy of Sciences.

[58]  L. Zangi,et al.  High-yield isolation, expansion, and differentiation of rat bone marrow-derived mesenchymal stem cells with fibrin microbeads. , 2006, Tissue engineering.

[59]  K. Landfester,et al.  Uptake of functionalized, fluorescent-labeled polymeric particles in different cell lines and stem cells. , 2006, Biomaterials.

[60]  L. Zangi,et al.  Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads , 2006, Bone Marrow Transplantation.

[61]  F. Barry,et al.  Endothelial progenitor cells: diagnostic and therapeutic considerations. , 2006, BioEssays : news and reviews in molecular, cellular and developmental biology.

[62]  J. Frank,et al.  Gadolinium-Fullerenol as a Paramagnetic Contrast Agent for Cellular Imaging , 2006, Investigative radiology.

[63]  D. Strunk,et al.  Blood Monocytes Mimic Endothelial Progenitor Cells , 2006, Stem cells.

[64]  Kathryn Sharer,et al.  In vivo detection of single cells by MRI , 2006, Magnetic resonance in medicine.

[65]  D. Strunk,et al.  Stem Cell Therapy for Ischemic Heart Disease: Beginning or End of the Road? , 2006, Cell transplantation.

[66]  Chris Heyn,et al.  In vivo magnetic resonance imaging of single cells in mouse brain with optical validation , 2006, Magnetic resonance in medicine.

[67]  J. Bulte,et al.  MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy. , 2006, Radiology.

[68]  R. Handgretinger,et al.  Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. , 2006, Cytotherapy.

[69]  D. Prockop,et al.  Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. , 2006, Cytotherapy.

[70]  Arend Heerschap,et al.  Magnetic resonance tracking of dendritic cells in melanoma patients for monitoring of cellular therapy , 2005, Nature Biotechnology.

[71]  S. Müller,et al.  Exogenous High-Mobility Group Box 1 Protein Induces Myocardial Regeneration After Infarction via Enhanced Cardiac C-Kit+ Cell Proliferation and Differentiation , 2005, Circulation research.

[72]  M. Beckerle,et al.  Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt. , 2005, The Journal of clinical investigation.

[73]  D. Torella,et al.  Cardiac Stem Cells Possess Growth Factor-Receptor Systems That After Activation Regenerate the Infarcted Myocardium, Improving Ventricular Function and Long-Term Survival , 2005, Circulation research.

[74]  Raymond C. Boston,et al.  Dynamic Imaging of Allogeneic Mesenchymal Stem Cells Trafficking to Myocardial Infarction , 2005, Circulation.

[75]  Luigi Naldini,et al.  Tie2 identifies a hematopoietic lineage of proangiogenic monocytes required for tumor vessel formation and a mesenchymal population of pericyte progenitors. , 2005, Cancer cell.

[76]  G. Mancardi,et al.  Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy. , 2005, Blood.

[77]  Jeff W M Bulte,et al.  Hot spot MRI emerges from the background , 2005, Nature Biotechnology.

[78]  T. Takayama,et al.  Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion , 2005 .

[79]  D. Torella,et al.  Stem cells in the dog heart are self-renewing, clonogenic, and multipotent and regenerate infarcted myocardium, improving cardiac function. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[80]  Yi Zhang,et al.  Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells. , 2005, Blood.

[81]  M. Pittenger,et al.  Human mesenchymal stem cells modulate allogeneic immune cell responses. , 2005, Blood.

[82]  C. Lange,et al.  Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. , 2005, American journal of physiology. Renal physiology.

[83]  H. L. Roh,et al.  Decreased Osteogenic Differentiation of Mesenchymal Stem Cells in Alcohol-induced Osteonecrosis , 2005, Clinical orthopaedics and related research.

[84]  F. Marini,et al.  Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. , 2005, Cytotherapy.

[85]  Nicolas Grenier,et al.  In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. , 2004, Radiology.

[86]  Jeff W M Bulte,et al.  Chondrogenic differentiation of mesenchymal stem cells is inhibited after magnetic labeling with ferumoxides. , 2004, Blood.

[87]  Jeff W M Bulte,et al.  Iron oxide MR contrast agents for molecular and cellular imaging , 2004, NMR in biomedicine.

[88]  Jeff W M Bulte,et al.  Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis , 2004, NMR in biomedicine.

[89]  S. Dimmeler,et al.  Endothelial Progenitor Cells: Characterization and Role in Vascular Biology , 2004, Circulation research.

[90]  D. Persons,et al.  Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[91]  Ulrich Göbel,et al.  bloodjournal.hematologylibrary.org at PENN STATE UNIVERSITY on February 20, 2013. For personal use , 2004 .

[92]  Moustapha Hassan,et al.  Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells , 2004, The Lancet.

[93]  M. Bianchi,et al.  Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation , 2004, The Journal of cell biology.

[94]  J. Isner,et al.  Endothelial Progenitor Cells , 2004 .

[95]  D. Krause,et al.  Plasticity of marrow-derived stem cells. , 2003, Blood.

[96]  Jeff W M Bulte,et al.  Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. , 2003, Radiology.

[97]  Naftali Kaminski,et al.  Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[98]  Ergin Atalar,et al.  In Vivo Magnetic Resonance Imaging of Mesenchymal Stem Cells in Myocardial Infarction , 2003, Circulation.

[99]  G. Seitz,et al.  Identification of a novel class of human adherent CD34- stem cells that give rise to SCID-repopulating cells. , 2003, Blood.

[100]  R. Handgretinger,et al.  Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells , 2003, Bone Marrow Transplantation.

[101]  L. Kanz,et al.  Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. , 2003, Haematologica.

[102]  P. Hernigou,et al.  Treatment of Osteonecrosis With Autologous Bone Marrow Grafting , 2002, Clinical orthopaedics and related research.

[103]  C. Verfaillie,et al.  Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. , 2002, Experimental hematology.

[104]  L. Muul,et al.  Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[105]  V. Soumelis,et al.  Dendritic cell lineage, plasticity and cross-regulation , 2001, Nature Immunology.

[106]  P. Anversa,et al.  Evidence that human cardiac myocytes divide after myocardial infarction. , 2001, The New England journal of medicine.

[107]  W. Koo,et al.  Clinical responses to bone marrow transplantation in children with severe osteogenesis imperfecta. , 2001, Blood.

[108]  E. Horwitz,et al.  Bone marrow mesenchymal cells: biological properties and clinical applications. , 2001, Journal of biological regulators and homeostatic agents.

[109]  Castro Fp,et al.  Core decompression and conservative treatment for avascular necrosis of the femoral head: a meta-analysis. , 2000 .

[110]  Darwin J. Prockop,et al.  Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta , 1999, Nature Medicine.

[111]  P. Hernigou,et al.  Decrease in the mesenchymal stem-cell pool in the proximal femur in corticosteroid-induced osteonecrosis. , 1999, The Journal of bone and joint surgery. British volume.

[112]  W. Hayes,et al.  Bone regeneration by implantation of purified, culture‐expanded human mesenchymal stem cells , 1998, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[113]  R. Class,et al.  Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[114]  D. Prockop.  Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues , 1997, Science.

[115]  O. Bagasra,et al.  Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[116]  Arnold I. Caplan,et al.  Mesenchymal Stem Cells , 1991, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[117]  A. Friedenstein,et al.  Stromal stem cells: marrow-derived osteogenic precursors. , 1988, Ciba Foundation symposium.

[118]  A. Friedenstein,et al.  Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. , 1968, Transplantation.