Contribution of the VH11 gene family to mitogen‐responsive B cell repertoire in C57BL/6 mice

The contribution of VH11 gene family to the development of the primary B cell repertoire has been studied by analyzing 1.8 × 104 mitogen induced B lymphocyte colonies. The data demonstrate that VH11 family is predominantly expressed among neonatal splenic as well as adult peritoneal B cell colonies, both rich in Ly‐1+ B cells. VH11 gene family expression among B splenocytes decreases during ontogeny and VH11 family pairs stochastically with different Vx families among mitogen‐activated neonatal B cell colonies, which are representative of an antigen unselected B cell repertoire. Thus, an increased VH11 expression among peritoneal and neonatal B cells points towards its biased expression among Ly‐1+ B lymphocytes. The restricted V gene rearrangements and VH11‐Vx9 pairing observed among anti‐bromelain‐treated mouse red blood cells autoantibodies are likely to be an outcome of both intrinsic gene recombination processes per se as well as selection by an autoantigen and/or local selective environmental factors.

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