The Effects of Artemisia spicigera Essential Oil before and after Flowering against Bovine Mastitis Bacteria

The absence of side effects of herbal essential oils and their antimicrobial effects has led researchers to evaluate the antimicrobial effects of essential oils and plant extracts in vitro to control microorganisms with a natural source. Artemisia is one of the genera of the Anthemideae family, widely distributed in the world and containing more than 400 species. In this study, Artemisia spicigera before and after flowering was prepared from region Bostanabad of East Azerbaijan and essential oils were extracted using Clevenger apparatus. Constituents of essential oil was analyzed by GC/MS. Then, the antimicrobial effect of essential oil Artemisia spicigera against bacteria isolated from clinical cases of bovine mastitis (Staphylococcus aureus and Escherichia coli) was determined and with Int. J. Food Nutr. Saf. 2018, 9(1): 1-7 Copyright © 2018 by Modern Scientific Press Company, Florida, USA 2 some standard antibiotics as a positive control was compared. For the preparation of bacteria of 20 cows with clinical mastitis was sampled and after culturing milk samples, the bacteria were isolated. The results showed that the inhibitoriest effect of Artemisia essential oils, before and after flowering, was 15 and 30 mm on Staphylococcus aureus and 9 and 25 mm on Escherichia coli in 100% concentration, respectively. Also in antibiogram method most diameter of inhibition zone in culture medium against Staphylococcus aureus and Escherichia coli was related to ceftriaxone, 40 and 25 mm, respectively. Results also showed that the antimicrobial effect of essential oils of Artemisia spicigera after flowering was more than before flowering. Due to the significant antibacterial activity of Artemisia essential oil in compared to different antibiotics can be concluded that essential oil of Artemisia spicigera has impressive antimicrobial properties, so it can be used in combination with other preservatives as natural herbal medicine using in treatment of mastitis in cows.

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