Effects of Undecylenic Acid Released from Denture Liner on Candida Biofilms

Denture liners (DL) are easily colonized by Candida spp. In an attempt to prevent biofilm colonization, manufacturers have incorporated undecylenic acid (UDA) into DL. In this in vitro study, the effects of UDA released from DL on Candida biofilms were investigated. The concentrations of UDA released from commercial DL were determined by gas chromatography–mass spectrometry (GC-MS). Minimum inhibitory concentration (MIC) and minimum fungistatic concentration (MFC) tests were performed for C. albicans or C. glabrata, with UDA for comparison with the concentrations released from DL. Specimens of DL with (experimental group) and without UDA (control group) were fabricated, and Candida biofilms were developed on DL surfaces. Biofilms were evaluated by cell counts, metabolic activity, structure, and secretion of proteinase or phospholipase. The concentrations of UDA released were within the MIC and MFC ranges. In the presence of UDA, C. albicans biofilms were thinner and had lower numbers of viable and active cells, although no significant enzymatic changes were observed relative to the control group (p > 0.05). In contrast, C. glabrata biofilms exhibited higher cell counts and greater metabolic activity and also increased proteinase activity in the presence of UDA relative to the control group (p < 0.05). Overall, UDA did not prevent Candida biofilm formation.

[1]  Aline Pinheiro de Moraes,et al.  Effect of triazine derivative added to denture materials on a microcosm biofilm model. , 2012, Journal of biomedical materials research. Part B, Applied biomaterials.

[2]  S. Villas-Bôas,et al.  The metabolic basis of Candida albicans morphogenesis and quorum sensing. , 2011, Fungal genetics and biology : FG & B.

[3]  L. Samaranayake,et al.  Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub‐inhibitory concentrations of fluconazole , 2011, Mycoses.

[4]  K. Zomorodian,et al.  Assessment of Candida species colonization and denture-related stomatitis in complete denture wearers. , 2011, Medical mycology.

[5]  H. Akin,et al.  Effect of different surface treatments on tensile bond strength of silicone-based soft denture liner , 2011, Lasers in Medical Science.

[6]  P. Wright,et al.  Effect of antifungal gels incorporated into a tissue conditioning material on the growth of Candida albicans. , 2010, Gerodontology.

[7]  M. Ruhnke,et al.  The quorum‐sensing molecule E,E‐farnesol—its variable secretion and its impact on the growth and metabolism of Candida species , 2010, Yeast.

[8]  H. Yatani,et al.  Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans. , 2009, International journal of antimicrobial agents.

[9]  L. Samaranayake,et al.  Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases. , 2009, Journal of microbiological methods.

[10]  J. Pemán,et al.  In Vitro Activities of Echinocandins against Candida krusei Determined by Three Methods: MIC and Minimal Fungicidal Concentration Measurements and Time-Kill Studies , 2009, Antimicrobial Agents and Chemotherapy.

[11]  J. Bagg,et al.  Mixed Candida albicans and Candida glabrata populations associated with the pathogenesis of denture stomatitis. , 2008, Oral microbiology and immunology.

[12]  W. Crielaard,et al.  The effect of Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces. , 2008, Archives of oral biology.

[13]  J. Verran,et al.  Colonization and deterioration of soft denture lining materials in vivo. , 2008, The European journal of prosthodontics and restorative dentistry.

[14]  W. Crielaard,et al.  DEVELOPMENT OF CANDIDA-ASSOCIATED DENTURE STOMATITIS: NEW INSIGHTS , 2008, Journal of applied oral science : revista FOB.

[15]  Clinical,et al.  Reference method for broth dilution antifungal susceptibility testing of yeasts : Approved standard , 2008 .

[16]  L. Samaranayake,et al.  Improvement of XTT assay performance for studies involving Candida albicans biofilms. , 2008, Brazilian dental journal.

[17]  A. Dongari-Bagtzoglou,et al.  Candida glabrata: an emerging oral opportunistic pathogen. , 2007, Journal of dental research.

[18]  T. Taylor.  Annual Report of the American Board of Prosthodontics. , 2007, Journal of prosthodontics : official journal of the American College of Prosthodontists.

[19]  V. Dubey,et al.  A novel serine protease cryptolepain from Cryptolepis buchanani: purification and biochemical characterization. , 2006, Journal of agricultural and food chemistry.

[20]  Alistair J. P. Brown,et al.  Exposure of Candida albicans to antifungal agents affects expression of SAP2 and SAP9 secreted proteinase genes. , 2005, The Journal of antimicrobial chemotherapy.

[21]  J. Hodges,et al.  A protocol for determining the surface free energy of dental materials. , 2004, Dental materials : official publication of the Academy of Dental Materials.

[22]  L. Samaranayake,et al.  Candida glabrata, an emerging fungal pathogen, exhibits superior relative cell surface hydrophobicity and adhesion to denture acrylic surfaces compared with Candida albicans , 2002, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[23]  M. Ghannoum,et al.  Antifungal Resistance of Candidal Biofilms Formed on Denture Acrylic in vitro , 2001, Journal of dental research.

[24]  J. Dolan,et al.  Undecylenic Acid Inhibits Morphogenesis ofCandida albicans , 2000, Antimicrobial Agents and Chemotherapy.

[25]  B. Ersbøll,et al.  Quantification of biofilm structures by the novel computer program COMSTAT. , 2000, Microbiology.

[26]  C. J. Morrison,et al.  Enhanced Extracellular Production of Aspartyl Proteinase, a Virulence Factor, by Candida albicans Isolates following Growth in Subinhibitory Concentrations of Fluconazole , 2000, Antimicrobial Agents and Chemotherapy.

[27]  M. Ghannoum.  Potential role of phospholipases in virulence and fungal pathogenesis. , 2000, Clinical microbiology reviews.

[28]  N. Gow,et al.  Cytoplasmic alkalinization during germ tube formation in Candida albicans. , 1988, Journal of general microbiology.