Predicting the Solubility of Recombinant Proteins in Escherichia coli

[1]  Catherine H. Schein,et al.  Solubility as a Function of Protein Structure and Solvent Components , 1990, Bio/Technology.

[2]  Catherine H. Schein,et al.  Production of Soluble Recombinant Proteins in Bacteria , 1989, Nature Biotechnology.

[3]  J. King,et al.  Protein Folding Intermediates and Inclusion Body Formation. , 1989, Bio/Technology.

[4]  E. Kawasaki,et al.  Renaturation and Purification of Biologically Active Recombinant Human Macrophage Colony-Stimulating Factor Expressed in E. Coli , 1989, Bio/Technology.

[5]  M. Mccaman.  Fragments of prochymosin produced in Escherichia coli form insoluble inclusion bodies , 1989, Journal of bacteriology.

[6]  B. Slatko,et al.  An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. , 1988, Gene.

[7]  A. Sommer,et al.  Production and characterization of human basic fibroblast growth factor from Escherichia coli. , 1988, The Journal of biological chemistry.

[8]  W. P. Dixon,et al.  BMPD statistical software manual , 1988 .

[9]  M. de Pedro,et al.  A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli , 1988, Journal of bacteriology.

[10]  C. Dykes,et al.  Expression of atrial natriuretic factor as a cleavable fusion protein with chloramphenicol acetyltransferase in Escherichia coli. , 1988, European journal of biochemistry.

[11]  W. Sirawaraporn,et al.  Heterologous expression of the bifunctional thymidylate synthase-dihydrofolate reductase from Leishmania major. , 1988, Biochemistry.

[12]  M. Piatak,et al.  Expression of soluble and fully functional ricin A chain in Escherichia coli is temperature-sensitive. , 1988, The Journal of biological chemistry.

[13]  J. King,et al.  Formation of aggregates from a thermolabile in vivo folding intermediate in P22 tailspike maturation. A model for inclusion body formation. , 1988, The Journal of biological chemistry.

[14]  K. Nagai,et al.  Refolding and crystallographic studies of eukaryotic proteins produced in Escherichia coli. , 1988, Biochemical Society transactions.

[15]  C. Schein,et al.  Formation of Soluble Recombinant Proteins in Escherichia Coli is Favored by Lower Growth Temperature , 1988, Bio/Technology.

[16]  C. Richardson,et al.  Gene 19 of bacteriophage T7. Overexpression, purification, and characterization of its product. , 1988, The Journal of biological chemistry.

[17]  G. Buell,et al.  Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion , 1987, Journal of bacteriology.

[18]  W. Bishai,et al.  High-level expression of a proteolytically sensitive diphtheria toxin fragment in Escherichia coli , 1987, Journal of bacteriology.

[19]  C. Dobson,et al.  Proline isomerism in staphylococcal nuclease characterized by NMR and site-directed mutagenesis , 1987, Nature.

[20]  M. Weir,et al.  Purification and renaturation of recombinant human interleukin-2. , 1987, The Biochemical journal.

[21]  Satish K. Sharma,et al.  FOlding and Activation of Recombinant Human Prorenin , 1987, Biotechnology and applied biochemistry.

[22]  F. Marston.  The purification of eukaryotic polypeptides synthesized in Escherichia coli. , 1986, The Biochemical journal.

[23]  H. Fujisawa,et al.  Overproduction and purification of the products of bacteriophage T3 genes 18 and 19, two genes involved in DNA packaging. , 1986, Virology.

[24]  A. Schreiber,et al.  A general method for retrieving the components of a genetically engineered fusion protein. , 1986, DNA.

[25]  D. Goeddel,et al.  Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[26]  T. Nishi,et al.  Cloning and expression of cDNA for salmon growth hormone in Escherichia coli. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[27]  M. Wigler,et al.  Purification and characterization of human H-ras proteins expressed in Escherichia coli , 1985, Molecular and cellular biology.

[28]  K. Williams,et al.  Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[29]  F. Marston,et al.  Examination of calf prochymosin accumulation in Escherichia coli: disulphide linkages are a structural component of prochymosin‐containing inclusion bodies. , 1985, The EMBO journal.

[30]  M. Barbacid,et al.  Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[31]  S. R. Kushner,et al.  Amplification and purification of plasmid-encoded thioredoxin from Escherichia coli K12. , 1984, The Journal of biological chemistry.

[32]  L. J. Perry,et al.  Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[33]  J. García,et al.  Overproduction and purification of the connector protein of Bacillus subtilis phage phi 29. , 1984, Nucleic acids research.

[34]  R. Lathe,et al.  High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[35]  J. Devereux,et al.  A comprehensive set of sequence analysis programs for the VAX , 1984, Nucleic Acids Res..

[36]  John D. Roberts,et al.  The bacteriopbage λ O replication protein: isolation and characterization of the amplified initiator , 1983 .

[37]  L. Enquist,et al.  An immunologically active chimaeric protein containing herpes simplex virus type 1 glycoprotein D , 1983, Nature.

[38]  R. Reed.  Transposon-mediated site-specific recombination: A defined in vitro system , 1981, Cell.

[39]  K. R. Woods,et al.  Prediction of protein antigenic determinants from amino acid sequences. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[40]  H. Heyneker,et al.  Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene. , 1980, Biochemistry.

[41]  J. Shine,et al.  Expression of cloned β-endorphin gene sequences by Escherichia coli , 1980, Nature.

[42]  P. Seeburg,et al.  Synthesis of growth hormone by bacteria , 1978, Nature.

[43]  A. Riggs,et al.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. , 1977, Science.

[44]  H. Lehmann,et al.  Haemoglobin Köln (β–98 Valine → Methionine): An Unstable Protein Causing Inclusion-Body Anaemia , 1966, Nature.

[45]  Charles Tanford,et al.  Physical Chemistry of Macromolecules , 1961 .

[46]  R. Fisher.  THE USE OF MULTIPLE MEASUREMENTS IN TAXONOMIC PROBLEMS , 1936 .

[47]  H. E. Umbarger,et al.  Escherichia coli and Salmonella typhimurium. Cellular and molecular biology. Volumes I and II. , 1987 .

[48]  T. Vanaman,et al.  New Approaches to the Expression and Isolation of a Regulatory Protein , 1986 .

[49]  J. Dodt,et al.  Chemical synthesis and expression of a gene coding for hirudin, the thrombin-specific inhibitor from the leech Hirudo medicinalis. , 1986, Biological chemistry Hoppe-Seyler.

[50]  T. Arakawa,et al.  Theory of protein solubility. , 1985, Methods in enzymology.

[51]  W. Fiers,et al.  High-level synthesis in Escherichia coli of the SV40 small-t antigen under control of the bacteriophage lambda pL promoter. , 1982, Gene.

[52]  H. Heyneker,et al.  PRODUCTION OF BIOLOGICALLY ACTIVE Nα-DESACETYL THYMOSIN α1 IN ESCHERICHIA COLI THROUGH EXPRESSION OF A CHEMICALLY SYNTHESIZED GENE , 1981 .

[53]  A. Riggs,et al.  Expression in Escherichia coli of chemically synthesized genes for human insulin. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[54]  P. Y. Chou,et al.  Empirical predictions of protein conformation. , 1978, Annual review of biochemistry.

[55]  W. Kauzmann.  Some factors in the interpretation of protein denaturation. , 1959, Advances in protein chemistry.