Brillouin microspectroscopy assessment of tissue differentiation during embryonic development

Changes in mechanical properties represent one of the driving factors behind cell differentiation during embryonic development. However, measuring these changes without disrupting the normal progression of morphogenesis or destroying the developing organism is not trivial. Brillouin microspectroscopy has been shown to be capable of nocontact, non-destructive and non-disruptive assessment of elastic properties in developing zebrafish embryos. The present study builds upon the previous work, and observes the changes in elasticity during the development of heart and brain in zebrafish embryos from 8 to 28 hpf (hours post-fertilization) at regular intervals. Brillouin microspectroscopy has proved to be a suitable technique to continuously monitor tissue differentiation and the development of individual organs with high spatial resolution without harming the developing organism.

[1]  Zhaokai Meng,et al.  Brillouin spectroscopy reveals changes in muscular viscoelasticity in Drosophila POMT mutants , 2015, Photonics West - Biomedical Optics.

[2]  Lance A Davidson,et al.  Embryo mechanics: balancing force production with elastic resistance during morphogenesis. , 2011, Current topics in developmental biology.

[3]  Vladislav V. Yakovlev,et al.  Elasticity-based identification of tumor margins using Brillouin spectroscopy , 2016, SPIE BiOS.

[4]  Vladislav V. Yakovlev,et al.  Background clean-up in Brillouin microspectroscopy of scattering medium. , 2014, Optics express.

[5]  Vladislav V. Yakovlev,et al.  Stimulated Brillouin Scattering Microscopic Imaging , 2015, Scientific Reports.

[6]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[7]  Jinhyoung Park,et al.  Acoustic Radiation Force Impulse (ARFI) Imaging of Zebrafish Embryo by High-Frequency Coded Excitation Sequence , 2011, Annals of Biomedical Engineering.

[8]  Zhaokai Meng,et al.  Flow cytometry using Brillouin imaging and sensing via time-resolved optical (BISTRO) measurements. , 2015, The Analyst.

[9]  Marlan O Scully,et al.  Dual Raman-Brillouin Microscope for Chemical and Mechanical Characterization and Imaging. , 2015, Analytical chemistry.

[10]  Larry A Taber,et al.  Probing regional mechanical properties of embryonic tissue using microindentation and optical coherence tomography. , 2015, Methods in molecular biology.

[11]  Carl Paterson,et al.  Spectral broadening in Brillouin imaging , 2013 .

[12]  S. Yun,et al.  Brillouin optical microscopy for corneal biomechanics. , 2012, Investigative ophthalmology & visual science.

[13]  William J. Polacheck,et al.  Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy , 2015, Nature Methods.

[14]  P. Currie,et al.  Animal models of human disease: zebrafish swim into view , 2007, Nature Reviews Genetics.

[15]  Vladislav V. Yakovlev,et al.  Impulsive Brillouin microscopy , 2017 .

[16]  Vladislav V. Yakovlev,et al.  Watching embryonic development in a new light: elasticity specific imaging with dual Brillouin/Raman microspectroscopy , 2016, SPIE BiOS.

[17]  Zhaokai Meng,et al.  Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy , 2016, Journal of biophotonics.

[18]  Vincent Fleury,et al.  Measuring the micromechanical properties of embryonic tissues. , 2016, Methods.

[19]  Vladislav V. Yakovlev,et al.  Seeing cells in a new light: a renaissance of Brillouin spectroscopy , 2016 .

[20]  Graham R. Scott,et al.  Temperature during embryonic development has persistent effects on metabolic enzymes in the muscle of zebrafish , 2014, Journal of Experimental Biology.

[21]  Donald E Ingber,et al.  Mechanical control of tissue morphogenesis during embryological development. , 2006, The International journal of developmental biology.

[22]  S. Yun,et al.  In vivo Brillouin optical microscopy of the human eye , 2012, Optics express.

[23]  Zhaokai Meng,et al.  Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet‐induced obese rats , 2017, Journal of biophotonics.