Cryo-EM enters a new era

Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.

[1]  S. Scheres,et al.  Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles , 2013, eLife.

[2]  V. Ramakrishnan,et al.  Molecular Architecture of a Eukaryotic Translational Initiation Complex , 2013, Science.

[3]  Shaoxia Chen,et al.  Prevention of overfitting in cryo-EM structure determination , 2012, Nature Methods.

[4]  D. Julius,et al.  TRPV1 structures in distinct conformations reveal mechanisms of activation , 2013, Nature.

[5]  Yanyu Zhao,et al.  Three-dimensional structure of human γ-secretase , 2014, Nature.

[6]  D. Julius,et al.  Structure of the TRPV1 ion channel determined by electron cryo-microscopy , 2013, Nature.

[7]  S. Scheres,et al.  Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine , 2014, eLife.

[8]  Israel S. Fernández,et al.  Structure of the Mammalian Ribosome-Sec61 Complex to 3.4 Å Resolution , 2014, Cell.

[9]  R. Henderson,et al.  Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy. , 2003, Journal of molecular biology.

[10]  Alan Brown,et al.  Structure of the Yeast Mitochondrial Large Ribosomal Subunit , 2014, Science.

[11]  Sjors H.W. Scheres,et al.  A Bayesian View on Cryo-EM Structure Determination , 2012, 2012 9th IEEE International Symposium on Biomedical Imaging (ISBI).

[12]  R. Henderson.  The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules , 1995, Quarterly Reviews of Biophysics.

[13]  W. Kühlbrandt,et al.  Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector , 2014, eLife.

[14]  D. Agard,et al.  Electron counting and beam-induced motion correction enable near atomic resolution single particle cryoEM , 2013, Nature Methods.

[15]  S. Scheres.  Beam-induced motion correction for sub-megadalton cryo-EM particles , 2014, eLife.

[16]  W. Kühlbrandt.  The Resolution Revolution , 2014, Science.