Optical Sensors for Ions and Protein Based on Digital Color Analysis

A novel colorimetric method, digital color analysis (DCA), was proposed using a digital color analyzer and was applied to various quantitative analyses using chromaticity coordinates and suitable sensors for visual colorimetry based on the characteristics of human visual perception by virtual simulations based on digital color information. On the basis of DCA, we developed a visual colorimetric sensor for Li+, NH4+ and protein determination by the mixing of two kinds of lipophilic dyes, whose optimum mixing ratio was obtained by virtual simulation based on DCA. Using DCA, a linear relation calibration curve can be obtained over the wide range of Li+, NH4+ and protein concentrations and this indicated a different color change with increase of the concentrations of these substances. These color sensors based on DCA have great potential for this analytical purpose.

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