Effect of Cerebral Intraventricular Insulin on Pancreatic Insulin Secretion in the Dog

The effect of cerebral intraventricular insulin on pancreatic insulin secretion was investigated. An extracorporeal pancreatic blood circuit was established after laparotomy to monitor blood flow and insulin concentration directly from the superior pancreaticoduodenal vein. Phentolamine was infused throughout (0.2 mg./min. intravenously) to block alpha-adrenergic effects of any catecholamine secretion induced by surgical stress. Glucose (1.5 mg./kg./min. intravenously) was infused to maintain a constant baseline stimulation of insulin secretion. Six dogs received insulin and six control dogs received saline through a spinal needle stereotaxically placed into the left lateral cerebral ventricle. After central injection of insulin (0.2 U./kg.) there was a significant increase of pancreatic output as early as five minutes. It is concluded that the pancreatic beta-cells are under the influence of insulin-sensitive cells of the CNS.

[1]  S. Woods,et al.  Effect of Intracisternal Insulin on Plasma Glucose and Insulin in the Dog , 1975, Diabetes.

[2]  R. Bergman,et al.  Direct enhancement of insulin secretion by vagal stimulation of the isolated pancreas. , 1973, The American journal of physiology.

[3]  D. Porte,et al.  Control of insulin secretion by catecholamines, stress, and the sympathetic nervous system. , 1973, Federation proceedings.

[4]  G. Mogenson,et al.  Blood glucose, insulin, and free fatty acids after stimulation and lesions of the hypothalamus. , 1972, The American journal of physiology.

[5]  L. Frohman,et al.  Effect of hypothalamic stimulation on plasma glucose, insulin, and glucagon levels. , 1971, The American journal of physiology.

[6]  C. J. Goodner,et al.  Studies of substrate regulation in fasting. II. Effect of infusion of glucose io the carotid artery upon fasting lipolysis in the baboon. , 1969, The Journal of clinical investigation.

[7]  R. Melzack,et al.  Effects of glucose on multi-unit activity in the hypothalamus. , 1969, Experimental neurology.

[8]  D. Anderson,et al.  Chicken cerebrospinal fluid: normal composition and response to insulin administration , 1969, The Journal of physiology.

[9]  T. Ono,et al.  Glucose and Osmosensitive Neurones of the Rat Hypothalamus , 1969, Nature.

[10]  K. Yada,et al.  [Immunoassay of insulin]. , 1968, Rinsho byori. The Japanese journal of clinical pathology.

[11]  R. U. Margolis,et al.  Effect of Intracisternally Administered Insulin-131I in Normal and Vagotomized Dogs∗ , 1968, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[12]  T. Shimazu,et al.  Reciprocal Influences of the Ventromedial and Lateral Hypothalamic Nuclei on Blood Glucose Level and Liver Glycogen Content , 1966, Nature.

[13]  S. Lavy,et al.  Effect of insulin administered intracisternally on the glucose level of the blood and the cerebrospinal fluid in vagotomized dogs. , 1966, Experimental neurology.

[14]  A. Lazarow,et al.  Immunoassay of Insulin: Two Antibody System: Plasma Insulin Levels of Normal, Subdiabetic and Diabetic Rats , 1963, Diabetes.

[15]  S. Lavy,et al.  Effect of insulin administered intracisternally in dogs on the glucose level of the blood and the cerebrospinal fluid , 1961 .

[16]  W. Hoffman.  A RAPID PHOTOELECTRIC METHOD FOR THE DETERMINATION OF GLUCOSE IN BLOOD AND URINE , 1937 .

[17]  D. Porte,et al.  Neural regulation of insulin secretion in the dog. , 1973, The Journal of clinical investigation.

[18]  A. Goldberg,et al.  The influence of cholinomimetics upon insulin secretion. , 1968, Diabetes.

[19]  T. Kuzuya.  [Regulation of insulin secretion by the central nervous system. II. The role of the hypothalamus and the pituitary gland upon insulin secretion]. , 1962, Japanese journal of medical science & biology.