Mitochondrial DNA A3243G mutation in noise-induced sensorineural hearing loss

─ Abstract ─ Objectives : A different sequence change, in the mitochondrial tRNA LEU(UUR) gene, has been proposed as a candidate mutation in the sensorineurnal hearing loss. The purpose of current study is to identify the association between the noise-induced sensorineurnal hearing loss and the A to G mutation at nucleotide 3243 of mitochondrial DNA. Methods : Subjects were established by history and chart review, and audiological and clinical data were obtained. Blood was sampled from 101 controls, 50 noise-induced hearing loss, and 12 sensorineural deafness. The DNA of these individuals was extracted, and mitochondrial genome was analyzed by polymerase chain reaction. Subsequently, the cod-ing sequence of mitochondrial genome was sequenced, and compared to the normal sequence, and all sequence variations were analyzed by restriction endonuclease ApaI. Results : Mitochondrial DNA mutation (3243A->G) was not detected by polymerase chain reaction (PCR) in any patients with noise-induced hearing loss, sensorineural hearing loss, and normal control without hearing loss in Koreans. The DNA sequencing of PCR products did not revealed an A to G substitution at nucleotide 3243 of mitochondrial DNA. Conclusions : The noise-induced sensorineural hearing loss was not associated with mitochondrial DNA mutation (3243A->G).

[1]  R. Ramsden,et al.  Hearing Loss , 2017, Quick Reference Guide to Pediatric Care.

[2]  K. Abe,et al.  Mitochondrial DNA Deletion Is a Predisposing Cause for Sensorineural Hearing Loss , 1998, The Laryngoscope.

[3]  M. Seidman,et al.  Association of Mitochondrial DNA Deletions and Cochlear Pathology: A Molecular Biologic Tool , 1996, The Laryngoscope.

[4]  Rickie R. Davis,et al.  Genetics of age-related hearing loss in mice. III. Susceptibility of inbred and F1 hybrid strains to noise-induced hearing loss , 1996, Hearing Research.

[5]  T. Takasaka,et al.  Bilateral Sensorineural Hearing Loss Associated With the Point Mutation in Mitochondrial Genome , 1996, The Laryngoscope.

[6]  T. Prezant,et al.  Mitochondrial mutation associated with nonsyndromic deafness. , 1995, American journal of otolaryngology.

[7]  T. Takasaka,et al.  Ion transport mechanisms in the outer hair cell of the mammalian cochlea , 1994, Progress in Neurobiology.

[8]  E. Borg,et al.  Auditory degeneration after acoustic trauma in two genotypes of mice , 1993, Hearing Research.

[9]  K. Majamaa,et al.  Adult‐onset diabetes mellitus and neurosensory hearing loss in maternal relatives of MELAS patients in a family with the tRNALeu(UUR) mutation , 1993, Neurology.

[10]  M. Pembrey,et al.  Diabetes mellitus associated with a pathogenic point mutation in mitochondrial DNA , 1992, The Lancet.

[11]  D. Wallace,et al.  Mitochondrial genetics: principles and practice. , 1992, American journal of human genetics.

[12]  D. Wallace,et al.  Maternally transmitted diabetes and deafness associated with a 10.4 kb mitochondrial DNA deletion , 1992, Nature Genetics.

[13]  D. Wallace,et al.  Hypoxemia is associated with mitochondrial DNA damage and gene induction. Implications for cardiac disease. , 1991, JAMA.

[14]  I. Nonaka,et al.  A mutation in the tRNALeu(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies , 1990, Nature.

[15]  A. H. V. Schapira,et al.  MITOCHONDRIAL COMPLEX I DEFICIENCY IN PARKINSON'S DISEASE , 1989, The Lancet.

[16]  D. Robertson,et al.  Variability of noise-induced damage in the guinea pig cochlea: Electrophysiological and morphological correlates after strictly controlled exposures , 1983, Hearing Research.

[17]  F. Matschinsky,et al.  II Quantitative Study of Selected Enzymes Involved in Energy Metabolism of the Cochlear Duct , 1970, The Annals of otology, rhinology, and laryngology.

[18]  W BURNS,et al.  STUDY OF NOISE AND HEARING IN JUTE WEAVING. , 1965, The Journal of the Acoustical Society of America.

[19]  健 喜多村.  Mitochondrial DNA Mutation at Nucleotide 1555 in a Patient with Bilateral Sensorineural Hearing Loss of Unknown Etiology , 1997 .

[20]  H. S. Li.  Genetic influences on susceptibility of the auditory system to aging and environmental factors. , 1992, Scandinavian audiology. Supplementum.

[21]  E Borg,et al.  Age-related loss of auditory sensitivity in two mouse genotypes. , 1991, Acta oto-laryngologica.

[22]  N. Arnheim,et al.  Detection of a specific mitochondrial DNA deletion in tissues of older humans. , 1990, Nucleic acids research.

[23]  R. Franke,et al.  CAN TTS BE AN INDICATOR FOR INDIVIDUAL SUSCEPTIBILITY TO PTS , 1986 .