Minnesota system corneal preservation.

The clinical and laboratory results with a modified Minnesota system of organ culture corneal preservation are presented. A refinement of our preservation technique using a closed system, as well as the addition of chondroitin sulphate to the medium is presented. Laboratory results show preservation of corneal endothelial integrity for at least 21 days with maintenance of normal corneal thickness. In addition, a 10-day quarantine system reduces the risk of donor contamination and secondary endophthalmitis. Preliminary results of the 34 degrees C and 4 degrees C closed Minnesota corneal preservation system using chondroitin sulphate show that it is safe and efficacious and allows intermediate to long-term maintenance of sterile thin tissue prior to corneal transplantation.

[1]  J. Nelson,et al.  Fungal contamination in organ culture. , 1983, Archives of ophthalmology.

[2]  D. Doughman,et al.  Penetrating keratoplasty following intermediate term organ culture corneal preservation at 37 degrees C. , 1983, Fortschritte der Ophthalmologie : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft.

[3]  H. D. Cavanagh,et al.  Endophthalmitis following penetrating keratoplasty. , 1983, Ophthalmology.

[4]  D. Doughman,et al.  Prolonged Donor Cornea Preservation In Organ Culture: Long‐Term Clinical Evaluation , 1982, Transactions of the American Ophthalmological Society.

[5]  Doughman Dj.  Prolonged donor cornea preservation in organ culture: long-term clinical evaluation. , 1980 .

[6]  P. A. Larsen,et al.  Torulopsis glabrata endophthalmitis after keratoplasty with an organ-cultured cornea. , 1978, Archives of ophthalmology.

[7]  W. M. Bourne,et al.  Organ-cultured corneal endothelium in vivo. , 1977, Archives of ophthalmology.

[8]  D. Doughman,et al.  Human corneal endothelial layer repair during organ culture. , 1976, Archives of ophthalmology.

[9]  R. Good,et al.  THE FATE OF EXPERIMENTAL ORGAN‐CULTURED CORNEAL XENOGRAFTS , 1976, Transplantation.

[10]  Kaufman He.  Corneal cryopreservation and its clinical application. , 1976 .

[11]  D. Doughman,et al.  Penetrating keratoplasty using 37 C organ cultured cornea. , 1976, Transactions. Section on Ophthalmology. American Academy of Ophthalmology and Otolaryngology.

[12]  H. Kaufman.  Corneal cryopreservation and its clinical application. , 1976, Transplantation proceedings.

[13]  T. Slappey.  Corneal preservation. , 1976, Transplantation proceedings.

[14]  J. Aquavella,et al.  Corneal preservation using M-K medium. , 1975, American journal of ophthalmology.

[15]  A. E. Maumenee,et al.  Intermediate-term corneal storage for penetrating keratoplasty. , 1975, American journal of ophthalmology.

[16]  D. Doughman,et al.  Ultrastructure of human organ-cultured cornea. II. Stroma and epithelium. , 1975, Archives of ophthalmology.

[17]  B. McCarey,et al.  Improved corneal storage for penetrating keratoplasties in man. , 1975, American journal of ophthalmology.

[18]  D. Doughman,et al.  The ultrastructure of human organ-cultured cornea. I. Endothelium. , 1974, Archives of ophthalmology.

[19]  D. Doughman,et al.  Endothelium of the human organ cultured cornea: an electron microscopic study. , 1973, Transactions of the American Ophthalmological Society.

[20]  F W STOCKER,et al.  The endothelium of the cornea and its clinical implications. , 1953, Transactions of the American Ophthalmological Society.